笼养和散养蛋鸡小肠细菌菌群区系的聚合酶链式反应-变性梯度凝胶电泳分析

发布时间：2018-06-28 09:51

本文选题：蛋鸡 + 笼养　；参考：《动物营养学报》2016年06期

【摘要】：本试验对笼养和散养蛋雏鸡和成年蛋鸡的小肠中细菌种类进行分析。取8周龄雏鸡和30周龄产蛋鸡整个小肠内容物,进行聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)分析,结合指纹克隆,研究鸡小肠细菌菌群的DNA指纹图谱。结果显示,共从蛋鸡的肠内容物中分离出39株细菌,有4个菌门,包括变形菌(2株,5.1%),拟杆菌(4株,10.2%),放线菌(5株,12.8%)和厚壁菌门(21株,53.8%),以及7个环境样品(不可培养细菌)。不同阶段蛋鸡不同肠段存在不同的细菌种类,在成年母鸡和自由放养鸡肠道中的细菌种类比雏鸡和笼养鸡丰富。所有分离细菌中,共分离得到10株乳酸菌,除陪伴粪球菌外,其余9株乳杆菌作为微生态制剂后备菌保存。结果提示,饲养模式和饲养阶段对蛋鸡肠道中细菌群落种类分布有很大影响,散养模式细菌群落更丰富,成年鸡较雏鸡肠道细菌多。
[Abstract]:The bacterial species in the small intestine of cage and scattered egg chicks and adult laying hens were analyzed in this experiment. The whole intestinal contents of 8-week-old and 30-week-old laying hens were collected and analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE). The results showed that 39 strains of bacteria were isolated from the intestinal contents of laying hens, including 4 phylum, including Proteus (2 strains, 5.1%), Bacteroides (4 strains, 10.2%), actinomycetes (5 strains, 12.8%), phylum platyphylla (21 strains, 53.8%), and 7 environmental samples (unculturable bacteria). There were different kinds of bacteria in different intestinal segments of laying hens at different stages, and the bacteria species in the intestine of adult hens and free-range chickens were more abundant than those of chicks and caged chickens. Among all the isolated bacteria, 10 lactic acid bacteria were isolated and 9 strains of Lactobacillus were preserved as microecological back-up bacteria. The results showed that the feeding mode and feeding stage had a great influence on the distribution of bacterial communities in the intestinal tract of laying hens.
【作者单位】：黑龙江八一农垦大学动物科技学院;黑龙江省动物疫病预防与控制中心;
【基金】：黑龙江省自然科学基金项目(C201444,C2015040) 黑龙江八一农垦大学校培育课题(XZR2014-05)
【分类号】：S831

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