瑶山鸡STAT5基因多态性及其与繁殖性状的关联性

发布时间：2018-06-29 17:30

本文选题：瑶山鸡 + STAT基因　；参考：《西南农业学报》2017年06期

【摘要】：【目的】为瑶山鸡繁殖性能的遗传改良、辅助育种提供理论依据。【方法】采用DNA测序技术,对瑶山鸡STAT5基因外显子的遗传多样性进行研究,并分析多态位点与繁殖性状的相关性。【结果】瑶山鸡STAT5基因存在11229 T→C、11298 T→C、10275 T→C、6571 T→C、6669 G→A、6732 G→A、6898 G→A、7142 G→A、7211 G→A、7219 G→A和7260 G→A等11个突变位点,其中,6898 G→A变异位点的杂合度和多肽信息含量最高,分别为0.5000和0.3750;7260 G→A变异位点的杂合度和多肽信息含量最低,分别为0.0950和0.0905;除6571 T→C、6732 G→A和7260 G→A 3个变异位点为低度多态外,其余8个变异位点均为中度多态;除6571 T→C和7260 G→A变异位点多态性与繁殖性状无显著性关联外,其余位点均与繁殖性状表现出显著(P0.05)或极显著(P0.01)相关。【结论】瑶山鸡STAT5基因多态性与瑶山鸡的繁殖性状具有一定的相关性,为进一步利用分子育种筛选瑶山鸡高产鸡群奠定了一定的基础。
[Abstract]:[objective] to provide theoretical basis for genetic improvement and auxiliary breeding of Yao Shan Chicken. [methods] genetic diversity of the exon of STAT5 gene in Yaoshan chicken was studied by DNA sequencing technique. The correlation between polymorphism loci and reproductive traits was analyzed. [results] there were 11 mutation loci in STAT5 gene of Yaoshan chicken, such as 11229 T / C ~ 11298 T ~ (1) C ~ (10) 275T ~ (10) T ~ (1) C ~ (661) T ~ (6) C ~ (666) G ~ (669) G ~ (?) ~ (669) G ~ (6) A ~ (6898) A ~ (72142G) A ~ (7219G) A and ~ (7260) G (A), etc. Among them, the heterozygosity and polypeptide information content were the highest at the mutation site of H6898G, which were 0.5000 and 0.3750,7260G, respectively, the lowest were the heterozygosity and the content of polypeptide information at the mutation site of 0.5000 and 0.3750,7260G, respectively. With the exception of the low polymorphic loci at 6571 T ~ (6571) T ~ (1) C ~ (6732) G and ~ (7260) G, the other 8 loci were moderately polymorphic, except that there was no significant correlation between the polymorphism of 6571 T ~ + C and ~ (7260) G ~ (1) A mutation sites and reproductive traits. All the other loci were significantly correlated with reproductive traits (P0.05) or extremely significant (P0.01). [conclusion] the polymorphism of STAT5 gene in Yaoshan Chicken has a certain correlation with reproductive traits of Yaoshan Chicken. It laid a foundation for screening high-yield chickens by molecular breeding.
【作者单位】：贵州省畜牧兽医研究所;
【基金】：贵州省农业科学院2014年博士基金项目“瑶山鸡STAT5变异检测及与产蛋性状的关联分析” 贵州省科技基金项目“瑶山鸡就巢性关键基因筛选”[黔科合J字(2014)2102]
【分类号】：S831.2

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