利用细菌性脑膜炎鼠模型评价侧脑室给药的治疗效果

发布时间：2018-06-30 00:43

本文选题：细菌脑膜炎 + 侧脑室给药　；参考：《上海交通大学》2015年硕士论文

【摘要】：侧脑室给药技术是一种新型的给药技术,不仅可以用于大脑中枢疾病的治疗,还广泛用于研究大脑心室的形成和形态发生以及描述大脑形态发生的突变体,并且侧脑室注射给药能直接将药物作用于大脑中枢系统,从而更有效的发挥了其治疗或损伤作用,在临床实验中具有较大的意义。目的:建立传染性胸膜放线杆菌引起的细菌性脑膜炎鼠模型,比较侧脑室和静脉注射连续3天给于青霉素对大鼠脑膜炎模型的治疗效果。实验方法:实验需使用16只雄性的成年SD大鼠且本次实验动物体重范围为250-300g,将其任意分成4个组,实验前2天选取其中一组进行脑部导管植入手术。实验时通过异氟烷将动物麻醉,用微量注射器作小脑延髓池穿刺,接种胸膜肺炎放线菌菌悬液,建立胸膜肺炎放线菌感染鼠的脑膜炎模型,给予不同给药剂量进行为期3天治疗,经侧脑室与静脉多次注射青霉素钠,通过对实验大鼠的存活率、临床症状的观察,脑病理组织学检查、细菌培养及聚合酶链反应(PCR)等技术对不同给药途径的效果评估。结果:大鼠接种传染性胸膜肺炎放线杆菌后,均出现脑膜炎临床症状;PCR实验检测结果呈阳性,证明脑脊液中检测到APP 7型菌株。大鼠细菌性脑膜炎模型制备成功。给药组大鼠临床症状较模型对照组有明显改善;侧脑室给予0.01mpk治疗效果要优于静脉注射10mpk,跟静脉注射62.5mpk组治疗效果大体相当。结论:侧脑室给药给药剂量低,治疗效果好,是一种理想的细菌性脑膜炎治疗方法。
[Abstract]:Lateral ventricle administration is a new kind of drug delivery technology. It can be used not only in the treatment of brain diseases, but also in the study of the formation and morphogenesis of the ventricles of the brain and the mutants describing the morphogenesis of the brain. The injection of the lateral ventricle can directly effect the drug on the central brain system, thus making it more effective. The effect of its treatment or injury is of great significance in clinical trials. Objective: to establish a bacterial meningitis rat model caused by contagious Actinobacillus pleurobacilli and compare the therapeutic effect of penicillin to the meningitis model of rats by penicillin for 3 days in the lateral ventricle and intravenous injection. Experimental methods should be used in 16 adult male SD rats. The weight range of the second experimental animal was 250-300g, which was randomly divided into 4 groups, and one of them was implanted in the brain 2 days before the experiment. In the experiment, the animals were anesthetized with isoflurane, the micro syringe was used as the cerebellum medullary pool, and the actinomycete suspension was inoculated to establish the meningitis model of the actinomycete infected mice. Type, given the dosage of different dosage for 3 days of treatment, through the lateral ventricle and intravenous injection of penicillin sodium, through the experimental rats, the survival rate, clinical symptoms, brain histopathology examination, bacterial culture and polymerase chain reaction (PCR) technique to evaluate the effect of different drug delivery methods. Results: the rats were inoculated with infectious pleural lung. The clinical symptoms of meningitis were found after Actinobacillus inflammatory. The results of PCR test were positive. It was proved that APP 7 was detected in the cerebrospinal fluid. The model of bacterial meningitis in rats was successfully prepared. The clinical symptoms of the rats in the administration group were obviously better than those in the model control group; the effect of 0.01mpk in the lateral ventricle was better than the intravenous injection of 10mpk and vein. Conclusion: the dosage of the lateral ventricle is low and the therapeutic effect is good. It is an ideal treatment method for bacterial meningitis. 62.5mpk is a safe and effective drug for the treatment of bacterial meningitis.
【学位授予单位】：上海交通大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S855.1

【参考文献】