猪A-FABP基因的克隆及其组织差异性表达

发布时间：2018-06-30 06:20

本文选题：猪 + A-FABP基因　；参考：《黑龙江畜牧兽医》2017年15期

【摘要】：为了进行猪A-FABP基因的克隆及不同器官组织中的差异表达,试验采用RT-PCR方法从长白猪背最长肌中克隆A-FABP基因的cDNA序列并进行测序比对,分别与人、牛、山羊、家鼠及鸡进行同源性比较,应用半定量RT-PCR方法检测猪心脏、肝脏、脾脏、肺脏、肾脏、背最长肌、腿肌中A-FABP基因的表达。结果表明:成功克隆得到A-FABP基因的cDNA序列,且比对结果为100%,该序列与人、牛、羊的同源性分别为90.98%、89.97%、89.97%,与鸡的同源性为73.68%。半定量RT-PCR结果为A-FABP基因在心脏、肝脏、脾脏、肺脏、肾脏、背最长肌、腿肌器官组织中均有表达,其中背最长肌和腿肌中A-FABP基因的表达量最高,肺脏内的表达量最低,A-FABP基因在动物进化中具有高度保守性,该基因在猪不同器官组织中的表达具有差异性。
[Abstract]:In order to clone the pig A-FABP gene and express it differently in different organs and tissues, the cDNA sequence of A-FABP gene was cloned from the longissimus dorsi muscle of Landrace pig by RT-PCR and sequenced and compared with human, cattle and goat, respectively. Homology was compared between domestic mice and chickens. The expression of A-FABP gene in pig heart, liver, spleen, lung, kidney, longissimus dorsi muscle and leg muscle was detected by semi-quantitative RT-PCR. The results showed that the cDNA sequence of A-FABP gene was cloned successfully, and the result of alignment was 100. The homology of this sequence with human, cattle and sheep was 90.989.97 and 89.97, respectively, and the homology with chicken was 73.68. The results of semi-quantitative RT-PCR showed that A-FABP gene was expressed in heart, liver, spleen, lung, kidney, longissimus dorsi muscle and leg muscle, and the expression of A-FABP gene was the highest in longissimus dorsi and leg muscle. The lowest expression of A-FABP gene in lung was highly conserved in animal evolution, and the expression of A-FABP gene was different in different organs and tissues of pig.
【作者单位】：青岛农业大学动物科技学院;
【基金】：转基因生物新品种培育科技重大专项项目(2016ZX08006-003) 山东省现代农业产业技术体系生猪创新团队建设项目(SDAIT-08-13)
【分类号】：S828

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