国内荷斯坦种公牛HH4遗传缺陷基因筛查

发布时间：2018-06-30 17:15

本文选题：荷斯坦牛 + 遗传缺陷　；参考：《中国畜牧杂志》2017年12期

【摘要】：HH4是新近在荷斯坦牛中发现的一种遗传缺陷,其分子机理为牛1号染色体上的GART基因编码区内的1个A/C错义突变。该遗传缺陷呈隐性遗传、突变等位基因纯合时,导致胚胎早期死亡。本研究针对HH4建立了基于PCR-RFLP技术的分子检测方法。结果表明:通过对466头国内荷斯坦种公牛进行基因筛查,发现6头携带者,均可以追溯到共同的祖先公牛Besne Buck。建议我国今后进口荷斯坦种牛时,对个体进行系谱分析和遗传缺陷基因分子检测,避免引入有害基因;同时,对国内现有荷斯坦公牛群体进行HH4筛查,明确标注每头公牛的携带状态,以便在生产实践中合理选种选配。
[Abstract]:HH4 is a newly discovered genetic defect in Holstein cattle. Its molecular mechanism is an A / C missense mutation in the coding region of the Gart gene on chromosome 1. The genetic defect is recessive and causes early embryo death when the mutant allele is homozygous. In this study, a molecular detection method based on PCR-RFLP was established for HH 4. The results showed that 6 carriers were found by genetic screening of 466 domestic Holstein bulls, all of which could be traced back to Besne Buckle, a common ancestor bull. In order to avoid the introduction of harmful genes, we should carry out pedigree analysis and genetic defect gene molecular detection in order to avoid the introduction of harmful genes. At the same time, we should carry out HH4 screening for the existing Holstein bull population in China. The carrying status of each bull is clearly marked in order to select species and match reasonably in production practice.
【作者单位】：中国农业大学动物科技学院;北京奶牛中心;北京市畜牧总站;
【基金】：国家科技支撑计划项目(2011BAD28B02) 北京市奶牛产业创新团队项目(BAIC06-2017) 现代农业(奶牛)产业技术体系建设专项资金(CARS-37)
【分类号】：S823.2

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