硒缓解鸡睾丸铅中毒的作用及机制研究

发布时间：2018-07-01 12:50

本文选题：醋酸铅 + 亚硒酸钠　；参考：《东北农业大学》2017年硕士论文

【摘要】：铅是有毒的重金属之一,在工业中被广泛应用。铅能引起环境污染,并且能在人类和动物体内蓄积,影响人类和动物健康。硒是人类和动物体内一种必需的营养微量元素,能缓解铅引起的毒性。本实验旨在研究硒对鸡睾丸铅中毒的缓解作用,为以后研究硒缓解鸟类睾丸铅中毒提供了参考。本试验将180只1日龄海兰蛋公鸡预饲1周后随机分成4组。对照组饲喂标准商品日粮(含硒为0.49mg/kg)和饮用水;硒组饲喂添加亚硒酸钠(含硒为1 mg/kg)的标准商品日粮和饮用水;铅组饲喂标准商品日粮和添加醋酸铅(含铅为350 mg/L)的饮用水;铅硒组饲喂添加亚硒酸钠的标准商品日粮(含硒为0.49 mg/kg)和添加醋酸铅的饮用水(含铅为350mg/L)。在试验的第30天、60天和90天,在每组中随机选取15只鸡处死并摘取睾丸组织。检测了30天、60天和90天的谷胱甘肽过氧化物酶(GPx)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、总抗氧化能力(T-AOC)、谷胱甘肽转移酶(GST)活力,谷胱甘肽(GSH)、丙二醛(MDA)和过氧化氢(H_2O_2)含量;一氧化氮(NO)含量和一氧化氮合酶(i NOS)活力;核转录因子(NF-κB)、环氧合酶-2(COX-2)、肿瘤坏死因子(TNF-α)、前列腺素E合成酶(PTGEs)、i NOS、白介素(IL)-2、IL-4、IL-6、IL-12β、IL-17和干扰素(IFN)-γ的m RNA水平、组织学和超微结构的变化;90天的铅和硒的浓度、NF-κB、i NOS、热休克蛋白(HSP)60、HSP70、HSP90蛋白水平和超微结构变化。得出如下结论:(1)铅中毒增加了睾丸组织铅浓度,降低了硒浓度,引起了睾丸铅积累;引起了鸡睾丸曲精细管和生殖细胞变形,部分生殖细胞散落到管腔,并且有炎性细胞浸润的出现。超微结构上发现,精母细胞核仁消失,间质细胞形状不规则,染色质边缘化以及线粒体空泡,引起了睾丸组织损伤;硒缓解了铅中毒诱导的上述变化,硒缓解了过量铅引起的鸡睾丸铅积累及睾丸损伤。(2)铅中毒上调了HSP60、HSP70和HSP90的蛋白质表达,硒缓解了铅中毒上调的鸡睾丸HSPs的蛋白质表达,结果说明铅中毒时,机体动员了热休克蛋白来抵御了铅的毒性,硒缓解了铅中毒。(3)铅中毒诱导了鸡睾丸NF-κB、COX-2、TNF-α、PTGEs、i NOS、IL-4、IL-6、IL-12β和IL-17 m RNA表达,NO含量和i NOS活力,诱导了NF-κB和i NOS蛋白表达,引起了炎症反应;降低了IL-2和IFN-γm RNA表达,引起了免疫抑制;硒缓解了铅中毒诱导的炎症相关因子表达的变化,缓解了鸡睾丸炎症反应和免疫抑制。(4)铅中毒降低了鸡睾丸SOD、GPx、CAT、T-AOC和GST活力,GSH含量,增加了MDA和H_2O_2含量,引起了氧化应激;硒缓解了铅中毒引起的氧化应激指标的变化,缓解了鸡睾丸氧化应激。
[Abstract]:Lead is one of the toxic heavy metals and is widely used in industry. Lead causes environmental pollution and accumulates in humans and animals, affecting human and animal health. Selenium is an essential nutrient trace element in human and animal, which can alleviate the toxicity caused by lead. The aim of this study was to study the effect of selenium on the remission of lead poisoning in the testis of chickens, and to provide a reference for the later study on the alleviation of lead poisoning in the testis of birds. 180 1-day-old Hailan egg cocks were randomly divided into 4 groups after 1 week of prefeeding. The control group was fed with standard commercial diet (selenium containing 0.49mg/kg) and drinking water, the selenium group was fed with standard commercial diet and drinking water supplemented with sodium selenite (containing 1 mg/kg of selenium), the lead group was fed with standard commercial diet and drinking water with lead acetate (350mg / L). The lead selenium group was fed with standard diet containing sodium selenite (0.49 mg/kg) and drinking water with lead acetate (350 mg / L lead). On the 30th day, 60 days and 90 days, 15 chickens were randomly selected to be killed and testicular tissues were removed. The contents of glutathione peroxidase (GPX), superoxide dismutase (SOD), catalase (cat), total antioxidant capacity (T-AOC), glutathione transferase (GST), glutathione (GSH), malondialdehyde (MDA) and hydrogen peroxide (H _ 2O _ 2) were measured at 30 and 90 days. Content of nitric oxide (no) and activity of nitric oxide synthase (iNOS), mRNA levels of nuclear transcription factor (NF- 魏 B), cyclooxygenase-2 (COX-2), tumor necrosis factor (TNF- 伪), prostaglandin E synthase (PTGEs), IL-6IL-12 尾 IL-17 and interferon (IFN)-纬, Histopathological and ultrastructural changes of lead and selenium concentrations NF- 魏 Bnos, heat shock protein (HSP60) HSP70HSP90 protein levels and ultrastructural changes were observed at 90 days. The conclusions are as follows: (1) lead poisoning increased lead concentration in testis, decreased selenium concentration, caused lead accumulation in testis, caused deformation of testicular tubules and germ cells, and scattered some germ cells into the lumen. And inflammatory cell infiltration appeared. Ultrastructural findings showed that spermatocyte nucleolus disappeared, interstitial cells were irregular in shape, chromatin was marginalized and mitochondria vacuole, which caused testicular tissue damage, selenium alleviated the above changes induced by lead poisoning. Selenium alleviated the lead accumulation and testicular damage induced by excessive lead. (2) lead poisoning upregulated the protein expression of HSP60, HSP70 and HSP90, and selenium alleviated the up-regulated protein expression of HSPs in the testis of lead poisoning. Heat shock protein was mobilized to resist lead toxicity and selenium alleviated lead poisoning. (3) lead poisoning induced the expression of nitric oxide (no), IL-6, IL-12 尾 and IL-17 mRNA, and induced the expression of NF- 魏 B and iNOS protein, and induced inflammatory reaction. Selenium alleviated the expression of inflammatory related factors induced by lead poisoning, and alleviated the inflammatory reaction and immunosuppression of chicken testis. (4) lead poisoning decreased the contents of T-AOC and GSH of T-AOC and GST activity in testis. The contents of MDA and H _ 2O _ 2 were increased and oxidative stress was induced by selenium. Selenium alleviated the changes of oxidative stress index caused by lead poisoning and alleviated oxidative stress in testis of chicken.
【学位授予单位】：东北农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S858.31

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