大孔吸附树脂纯化黄芪茎总皂苷的工艺及其抗氧化性

发布时间：2018-07-01 18:05

本文选题：黄芪茎 + 总皂苷　；参考：《饲料工业》2017年01期

【摘要】：以总皂苷吸附率、解吸率为考察指标,探索LSA-10型大孔吸附树脂纯化黄芪茎总皂苷的工艺条件及其体外抗氧化性。采用单因素和正交试验法对黄芪茎总皂苷的纯化工艺进行优化;采用分光光度法探索了黄芪茎总皂苷样品液对DPPH·、·OH的清除活性。试验结果表明,黄芪茎总皂苷的最佳纯化工艺条件为:以生药浓度为0.015 g/ml的黄芪茎样品液进行试验,吸附时间为4 h,样品液用量/树脂质量的比值为15 ml/g,解吸液乙醇体积分数为85%,解吸液用量/树脂质量的比值为15 ml/g,解吸时间为1 h。在上述试验条件下黄芪茎总皂苷样品液的平均吸附率和解吸率分别为90.68%和93.13%,纯化后的干浸膏总皂苷含量由5.94%提高到12.66%;黄芪茎总皂苷样品液对DPPH·、·OH具有较强的清除能力。因此,LSA-10型大孔吸附树脂对黄芪茎总皂苷具有富集作用,其优化的纯化工艺条件比较稳定;黄芪茎总皂苷提取液具有一定的抗氧化活性,而且其清除活性随着质量浓度的增加而逐渐增强。
[Abstract]:With the total saponins adsorption rate and desorption rate as the index, the process conditions of purification of total saponins from Astragalus membranaceus stem by LSA-10 macroporous adsorption resin and its antioxidation in vitro were explored. The purification process of total saponins of Astragalus membranaceus was optimized by single factor and orthogonal test, and the scavenging activity of total saponins of Astragalus membranaceus to DPPH and OH was studied by spectrophotometry. The results showed that the optimum purification conditions of total saponins of Astragalus membranaceus were as follows: the sample solution of Astragalus membranaceus with the concentration of 0.015 g/ml was tested. The adsorption time is 4 h, the ratio of sample amount to resin mass is 15 ml / g, the volume fraction of ethanol in desorption solution is 85, the ratio of desorption amount to resin mass is 15 ml / g, and the desorption time is 1 h. Under the above conditions, the average adsorption rate and desorption rate of total saponins of Astragalus membranaceus were 90.68% and 93.13%, respectively, and the total saponins content of dried extract was increased from 5.94% to 12.666.The sample solution of total saponins of Astragalus membranaceus had strong scavenging ability to DPPH and OH. Therefore, LSA-10 macroporous adsorption resin can enrich the total saponins of Astragalus membranaceus, and the optimized purification conditions are stable, and the extract of total saponins of Astragalus membranaceus has certain antioxidant activity. Moreover, the scavenging activity increased with the increase of mass concentration.
【作者单位】：吉林化工学院化学与制药工程学院;
【分类号】：S816.7

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