麦洼牦牛基因组三碱基重复微卫星的挖掘及其遗传多态性分析

发布时间：2018-07-01 18:54

本文选题：麦洼牦牛 + 三碱基　；参考：《中国畜牧兽医》2017年05期

【摘要】：为了解麦洼牦牛现有群体的遗传多样性及种群遗传分化特征,试验通过生物信息学方法从已公布的牦牛基因组中筛选到9 466个三碱基重复微卫星位点,以191个麦洼牦牛基因组DNA样品为模板对各个位点进行PCR扩增和聚丙烯酰胺凝胶电泳基因分型。经过哈迪-温伯格平衡检验,在15个三碱基微卫星位点中,有5个位点偏离哈迪-温伯格平衡(P0.05)。15个微卫星位点均为高度多态性(PIC0.5),群体平均观测杂合度(Ho)、平均期望杂合度(He)和平均多态信息含量(PIC)分别达到0.5041、0.8152和0.7831。进一步通过Structure 3.0软件分析发现,麦洼牦牛群体结构单一,无分化特征,表明麦洼牦牛品种虽然具有较高的遗传多态性但是无分化迹象。
[Abstract]:In order to understand the genetic diversity and population differentiation characteristics of the existing populations of Maiwa yak, 9,466 repeat microsatellite loci were selected from published yak genome by bioinformatics method. The genomic DNA samples of 191 Maiwa yaks were used as template for PCR amplification and polyacrylamide gel electrophoresis genotyping. After the Hardy-Weinberg equilibrium test, there were 15 tribasic microsatellite loci, Five loci deviated from the Hardiy-Weinberg equilibrium (P0.05). The 15 microsatellite loci were highly polymorphic (PIC0.5). The population average observed heterozygosity (Ho), average expected heterozygosity (he) and mean polymorphic information content (PIC) were 0.5041 and 0.7831, respectively. Further analysis of structure 3.0 software showed that the population structure of Maiwa yak was single and had no differentiation, which indicated that Maiwa yak had high genetic polymorphism but no differentiation.
【作者单位】：西南科技大学生命科学与工程学院;
【基金】：中国博士后科学基金资助项目(20090461336);中国博士后科学基金特别资助项目(201003703)
【分类号】：S823.85

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