动物益生菌短短芽胞杆菌FJAT-1501-BPA制剂的制备及其益生特性评价

发布时间：2018-07-01 19:54

  本文选题：短短芽胞杆菌 + 人工胃液　； 参考：《中国畜牧兽医》2017年10期

【摘要】：为优化短短芽胞杆菌FJAT-1501-BPA胶悬剂配方,获得性状稳定可长期贮存的菌剂,同时对其作为益生菌进行益生特性评价,本试验研究了不同稳定剂、p H、Na Cl及防腐剂浓度对短短芽胞杆菌FJAT-1501-BPA发酵液保存特性的影响,并对其产芽孢率、耐热性、抗生素敏感性、人工胃肠液耐受性、胆盐耐受性等生物学特性进行评估。结果显示,添加4%Na Cl、0.1‰柠檬酸钙、0.1‰双乙酸钠、0.1‰山梨酸钾、2.0‰黄原胶,调节p H为7,可作为短短芽胞杆菌FJAT-1501-BPA胶悬剂保存的参考条件。短短芽胞杆菌FJAT-1501-BPA能够抑制沙门氏菌、大肠杆菌、金黄色葡萄球菌3种动物肠道病原菌的生长,具有产孢率高、耐热、耐酸、耐胆盐的特点,可与链霉素、氨苄西林同时使用,益生效果良好。因此,短短芽胞杆菌FJAT-1501-BPA可作为益生菌菌株应用于畜牧生产。
[Abstract]:In order to optimize the formula of Bacillus short strain FJAT-1501-BPA gel suspension, a stable and long-term storage agent was obtained, and the probiotics were evaluated as probiotics. In this study, the effects of different stabilizers and preservative concentrations on the preservation characteristics of Bacillus brevis FJAT-1501-BPA fermentation broth were studied, and the spore-producing rate, heat resistance, antibiotic sensitivity and tolerance of artificial gastrointestinal fluid were also studied, and the effects of different stabilizers and preservatives on the preservation characteristics of the fermentation broth of Bacillus brevis FJAT-1501-BPA were studied. Bile salt tolerance and other biological characteristics were evaluated. The results showed that the addition of NaCl-0.1 鈥，

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