茶多酚对感染球虫肉鸡肠道微生态影响及其作用机理研究

发布时间：2018-07-02 09:30

本文选题：茶多酚 + 黄羽肉鸡　；参考：《安徽农业大学》2015年硕士论文

【摘要】：本试验在感染鸡球虫的黄羽肉鸡日粮中添加不同浓度水平的茶多酚(Tea Polyphenol,TP),通过测定黄羽肉鸡生长性能及肠道菌群、肠道形态、肠道黏膜通透性、血清生化指标、肠道内容物pH等肠道微生态相关指标和抗球虫指数(Anticoccidial index,ACI),较系统地研究了茶多酚对黄羽肉鸡感染球虫后生长性能的影响、对其肠道微生态的保护作用及机制和茶多酚抗球虫效果。试验选取体重相近、健康的黄羽肉仔鸡共210只(7日龄),随机分成7组,每组分为5个重复,每个重复6只鸡。其中以1组为对照组,试验2、6两组在基础日粮中添加350mg/kg的茶多酚,试验3、7两组在基础日粮中添加500 mg/kg的茶多酚,试验4组在基础日粮中添加500mg/kg的抗球虫药物二硝托胺预混剂。21日龄时,分别给第1组到第4组的每只试验鸡灌服5000个/ml的柔嫩艾美耳球虫卵囊悬液1ml。在试验鸡28日龄、35日龄采集样品并测定相关指标。试验结果表明:(1)茶多酚能够显著提高球虫感染肉鸡肠道中乳酸菌、双歧杆菌的含量,降低了大肠杆菌含量(P0.05),维护了肠道内正常的微生物区系;显著提高了血清中总蛋白、球蛋白的含量(P0.05),提高了肉鸡自身的免疫功能;显著降低了其血浆中细菌内毒素的含量(P0.05),有效减小了肠黏膜的通透性;显著降低了盲肠内容物的PH值(P0.05),增加了其肠道内有益菌的数量及其代谢产物的含量;显著改善了其小肠肠道形态,肠道各部位绒毛高度、V/C均显著高于对照组,尤其是十二指肠(P0.05),改善了肠道消化吸收功能;说明茶多酚可以维护球虫感染后肉鸡肠道微生态的动态平衡。(2)与对照组相比日粮中添加350mg/kg茶多酚时,平均日增重提高了8.82%(P0.05),料重比降低了7.9%(P0.05),添加500 mg/kg茶多酚时,平均日增重提高了6.73%(P0.05),料重比降低了6.23%(P0.05),说明茶多酚可以显著提高球虫感染黄羽肉鸡的生长性能(P0.05),但是添加量与生长性能之间不成正比。(3)与对照组相比,茶多酚试验组降低了试验鸡的血便记分,显著提高了相对增重率,350mg/kg茶多酚处理试验组和500mg/kg茶多酚处理试验组的ACI分别为173.95和172.90,说明日粮中添加茶多酚对柔嫩艾美耳球虫感染肉鸡具有一定抗球虫效果。
[Abstract]:Different concentrations of tea polyphenols (TP) were added to the diet of yellow broilers infected with coccidiosis. The growth performance, intestinal flora, intestinal morphology, intestinal mucosal permeability and serum biochemical indexes were measured. The effects of tea polyphenols on growth performance of yellow feather broilers infected with coccidiosis were studied systematically. The protective effect and mechanism of tea polyphenols on intestinal microecology and the effect of tea polyphenols on coccidiosis were studied. A total of 210 healthy yellow broilers (7 days old) of similar weight and weight were randomly divided into 7 groups. Each group was divided into 5 replicates and 6 chickens per replica. One group was used as the control group, two groups were treated with tea polyphenols of 350mg/kg in the basal diet and the other two groups were treated with 500 mg/kg of tea polyphenols in the basal diet. At the age of 21 days, the test 4 groups were fed with 5 000 ml of Eimeria tenella oocyst suspension from the first to the fourth groups respectively when they were added with the 500mg/kg anticoccidial drug dinitrotropine premixed agent in the basal diet at the age of 21 days, and each of the tested chickens in the first and fourth groups was given 1 ml of oocyst suspension of Eimeria tenella (Eimeria tenella) respectively. Samples were collected at 28 days old and 35 days old and related indexes were measured. The results showed that: (1) Tea polyphenols could significantly increase the contents of lactic acid bacteria and bifidobacterium in the intestinal tract of coccidiosis infected broilers, decrease the content of Escherichia coli (P0.05), maintain the normal microbial flora in the intestine, and significantly increase the total protein in serum. The content of globulin (P0.05) improved the immune function of broilers, significantly decreased the content of bacterial endotoxin in plasma (P0.05) and effectively reduced the permeability of intestinal mucosa. The PH value of caecum contents was significantly decreased (P0.05), the number of beneficial bacteria and the contents of metabolites in intestinal tract were increased, and the intestinal morphology was significantly improved, and the villi height of villi in each part of the intestine was significantly higher than that in the control group. Especially duodenum (P0.05) improved intestinal digestion and absorption function, indicating that tea polyphenols can maintain the dynamic balance of intestinal microecology after coccidiosis infection. (2) compared with the control group, when 350mg/kg tea polyphenols were added to the diet, The average daily gain increased by 8.82% (P0.05), the feed / weight ratio decreased by 7.9% (P0.05), and the average daily gain was increased by 8.82% (P0.05). The average daily gain increased by 6.73% (P0.05) and the feed / weight ratio decreased by 6.23% (P0.05), which indicated that tea polyphenols could significantly improve the growth performance of coccidiosis infected yellow feather broilers (P0.05), but the addition of tea polyphenols was not directly proportional to the growth performance. (3) compared with the control group, tea polyphenols could significantly improve the growth performance of yellow feather broilers infected with coccidiosis. The tea polyphenols test group reduced the blood stool score of the tested chickens. The relative weight gain rate of 50 mg / kg tea polyphenol treatment group and 500mg/kg tea polyphenol treatment group were 173.95 and 172.90 respectively, which indicated that tea polyphenols added in the diet had a certain anti-coccidiosis effect on Eimeria tenella infected broilers.
【学位授予单位】：安徽农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.31

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