不同生长时期梅花鹿鹿茸转录组分析

发布时间：2018-07-03 00:00

本文选题：梅花鹿 + 鹿茸　；参考：《畜牧兽医学报》2017年02期

【摘要】：本研究旨在探究不同生长时期梅花鹿鹿茸的转录组差异,丰富梅花鹿鹿茸转录组信息。选取5个生长时期(10、20、28、44、66d)的梅花鹿鹿茸组织作为试验样品,利用Illumina HiSeqTM2500高通量测序,并用测序评估、基因注释等生物信息学方法进行分析。结果,经过测序、转录本拼接获得了375 657条contigs,平均长度为688bp,329 946个unigenes,平均长度为534bp。通过比对Nr、Nt、Pfam、KOG/COG、Swiss-prot、KEGG、GO等7大数据库,90.07%unigenes得到了成功注释。其中39 674个(12.02%)基因成功注释到GO数据库,在level 2水平上共分为41个类别;17 732个(5.37%)基因成功注释到将KOG的26个类别中。对5个生长时期的鹿茸转录本文库进行比较分析,共发现了509个差异基因,其中407个差异表达基因成功注释到GO数据库中,主要为信号转导、氧化还原、转录调节、蛋白酶降解等生物学过程。其中转录调节基因PER1、EGR1的表达随着鹿茸的生长而增加,而GAS1则相反,随着鹿茸的生长而降低,推测PER1、EGR1、GAS1等转录因子在鹿茸生长过程中可能起着重要的调节作用。本研究利用高通量测序技术对不同生长时期的梅花鹿鹿茸组织进行了转录组测序和分析,筛选到了梅花鹿鹿茸在不同生长时期下的差异表达基因,并获得了差异表达基因的功能。
[Abstract]:The purpose of this study was to explore the transcriptional diversity of sika deer antler at different growth stages, and to enrich the information of sika deer antler transcriptome. Five sika deer antler tissues were selected as experimental samples. Illumina HiSeqTM2500 high-throughput sequencing method was used to analyze sika deer antler tissues. The results were analyzed by sequencing evaluation, gene annotation and other bioinformatics methods. Results: after sequencing, 375,657 contigswere obtained, with an average length of 688bp329,946 unigenes and an average length of 534bp. A successful annotation was obtained by comparing seven databases, such as NrNtPfamKOGR Swiss-protprotgo, KEGGG go and other 7 databases, such as 90.07unigenes. 39,674 (12.02%) genes were successfully annotated into go database, and a total of 17,732 (5.37%) genes were successfully annotated into 26 categories of KOG at level 2 level. A total of 509 differentially expressed genes were found in 5 matured velvet antler transcripts, of which 407 differentially expressed genes were successfully annotated into go database, mainly for signal transduction, redox and transcription regulation. Protease degradation and other biological processes. The expression of transcriptional regulatory gene PER1-EGR1 increased with the growth of velvet antler, whereas GAS1 decreased with the growth of velvet antler, suggesting that transcription factors such as PER1-EGR1-GAS1 might play an important role in the growth of velvet antler. In this study, high-throughput sequencing technique was used to sequence and analyze the transcription patterns of sika deer antler tissues at different growth stages, and the differentially expressed genes of sika deer antler at different growth stages were screened. The function of differentially expressed genes was obtained.
【作者单位】：中国农业科学院特产研究所特种经济动物分子生物重点实验室;
【基金】：基金项目:特种动物遗传资源创新团队(CAAS-ASTIP-201X-ISAPS)
【分类号】：S825

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