竹鼠源弗氏柠檬酸杆菌的分离鉴定及致病性分析

发布时间：2018-07-03 00:35

本文选题：竹鼠 + 弗氏柠檬酸杆菌　；参考：《畜牧兽医学报》2017年05期

【摘要】：为确定桂林某竹鼠养殖场患病竹鼠的病原,从发病竹鼠肝组织分离到一株细菌。根据形态学特征及16S rRNA基因序列分析,确定分离菌株为弗氏柠檬酸杆菌。进化分析显示,该菌与来自棉花红蜘蛛的弗氏柠檬酸杆菌遗传关系最近,但处于独立的分支上。毒力试验结果表明,该菌对成年小白鼠的LD_(50)为2.8×10~8 CFU,对10~20日龄的竹鼠LD_(50)为6.3×10~8 CFU。药敏试验结果显示,分离菌株对头孢曲松、庆大霉素、阿米卡星、氧氟沙星、左氧氟沙星药物高度敏感,对青霉素、头孢唑啉、氟苯尼考、林可霉素、强力霉素等有耐药性。病理组织学观察显示,发病竹鼠肺泡间隔增宽伴随炎性细胞浸润;肝组织血管扩张、淤血;脾被膜外大量坏死细胞;肾组织肾小管上皮细胞肿胀、颗粒变性。综上研究结果表明,弗氏柠檬酸杆菌是引起桂林竹鼠发病和致死的病原菌,分离菌株对竹鼠有较强的致病性。
[Abstract]:A bacterial strain was isolated from the liver of a bamboo mouse in a bamboo farm in Guilin. According to morphological characteristics and sequence analysis of 16s rRNA gene, the isolated strain was identified as Citrobacter flexneri. Evolutionary analysis showed that the strain had the closest genetic relationship with Citrobacter flexneri from cotton red spider, but it was an independent branch. The toxicity test showed that the LD50 of the strain was 2.8 脳 10 ~ (8) CFU for adult mice and 6.3 脳 10 ~ (8) CFU for 10 ~ (20) days old bamboo mice. The results showed that the isolates were highly sensitive to ceftriaxone, gentamycin, amikacin, ofloxacin, levofloxacin, and resistant to penicillin, cefazolin, florfenicol, lincomycin and doxycycline. Histopathological observation showed that the alveolar septum widening accompanied by inflammatory cell infiltration; hepatic vasodilation, congestion; a large number of necrotic cells outside the spleen; renal tubular epithelial cells swelling, granular degeneration. The results showed that Citrate flexneri was the pathogen that caused the occurrence and death of bamboo rats in Guilin, and the isolated strains had strong pathogenicity to bamboo rats.
【作者单位】：广西壮族自治区兽医研究所广西兽医生物技术重点实验室;
【基金】：广西水产畜牧局科技推广应用项目(桂渔牧科201452004) 广西基本科研业务费专项(桂科专项15-4)
【分类号】：S852.61

【相似文献】