基于RNA-Seq筛选鸭肠炎病毒感染鸭脾差异表达免疫相关基因

发布时间：2018-07-03 02:37

本文选题：RAN-Seq + 鸭肠炎病毒　；参考：《畜牧兽医学报》2017年02期

【摘要】：为挖掘鸭肠炎病毒(duck enteritis virus,DEV)感染鸭的差异表达免疫相关基因,本研究以DEV GZ株经腿部肌肉接种50d鸭后,于接种后66、90和114h采集鸭脾组织样本,提取总RNA,通过高通量RNA-seq技术测序,应用GO和KEGG数据库进行比对注释,并采用荧光定量PCR对部分差异表达基因进行验证,结果显示:DEV接种66h时鸭脾的差异表达基因有511个,参与免疫相关生物学过程的为70个,其中上调基因46个,下调基因24个;90h时差异表达基因有485个,参与免疫相关生物学过程的为64个,其中上调基因49个,下调基因15个;114h时差异表达基因有531个,参与免疫相关生物学过程的为66个,其中上调基因35个,下调基因31个。GO数据库分析显示,差异表达免疫相关基因主要涉及细胞黏附、抗原加工和呈递、补体激活等免疫反应过程;KEGG数据库分析显示,差异表达免疫相关基因主要富集在细胞黏附分子、ECM受体互作、PPAR等信号通路;荧光定量PCR对7个差异表达基因进行检测显示,差异表达基因相对表达量与RNA-Seq技术测序结果基本一致。这些结果为进一步阐明DEV感染机制和机体免疫应答机制奠定了基础。
[Abstract]:In order to express the differentially expressed immune related genes of duck enteritis virus (duck enteritis virus, DEV) infected ducks. After inoculating 50D ducks in the leg muscles of DEV GZ strain, the samples of duck spleen tissue were collected after inoculation with 66,90 and 114h, and the total RNA was extracted from DEV and 114h after inoculation. The partial differential expression gene was verified by light quantitative PCR. The results showed that there were 511 differentially expressed genes in the spleen of the duck when DEV was inoculated with 66h, and 70 were involved in the immune related biological processes, including 46 up-regulated genes and 24 down-regulation genes. There were 485 differentially expressed genes at 90h and 64 biological processes related to immunology, including up regulation 49 genes were down regulated by 49. There were 531 differentially expressed genes and 66 genes involved in immune related biological processes, including 35 up-regulated genes and 31 down-regulated.GO database analysis. The differential expression of immune related genes involved cell adhesion, antigen processing and presentation, complement activation and other immune responses; KEGG database The analysis showed that the differentially expressed immune related genes were mainly enriched in cell adhesion molecules, ECM receptor interaction, PPAR and other signaling pathways, and fluorescence quantitative PCR detected 7 differentially expressed genes, and the relative expression of differentially expressed genes was basically consistent with the sequencing results of RNA-Seq technology. These results could further elucidate the mechanism and mechanism of DEV infection. The mechanism of body immune response is established.
【作者单位】：贵州大学动物科学学院;贵州大学动物疫病研究所;贵州省动物疫病与兽医公共卫生重点实验室;
【基金】：国家自然科学基金项目(31260607;31560703) 贵州省优秀青年科学人才培养计划项目(黔科合人字[2013]25号) 贵州省百层次创新型人才项目(黔科合人才[2016]4009号) 贵州省科技创新人才团队建设项目(黔科合人才团队[2015]4016号)
【分类号】：S858.32

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