内蒙古地区马匹人工授精技术的应用研究

发布时间：2018-07-03 06:55

本文选题：马匹 + 精液品质　；参考：《内蒙古农业大学》2016年硕士论文

【摘要】：选择饲养条件相同,年龄6-8岁的不同品种马10匹,其中包括4匹纯血马、2匹布琼尼、4匹蒙古马进行了常规精液品质检查,另选择97匹空怀母马进行低剂量细管子宫体不同发情期输精,并在授精后14天采用便携式B超仪进行妊娠诊断等一系列人工授精实验。从而获得三个不同品种种公马的精液品质的数据和母马授精的最佳情期和利用B超仪诊断母马早期妊娠技术要领,并将这些方法和数据采用科学的手段,因地制宜的向牧区推广,从而提高牧区使用人工授精技术来提高马匹的受胎率。该实验获得的具体结果如下：1、不同品种种公马精液品质检测比较研究。3个品种中,从射精量方面来看,布琼尼马平均采精量极显著高于纯血马、蒙古马(P0.01)；从精液密度方面来看,纯血马的平均精液密度极显著高于布琼尼马、蒙古马(P0.01)；从总精子数来看,布琼尼种公马的最多,其次是蒙古马。从鲜精活力上看,蒙古马鲜精活力极显著高于纯血马、布琼尼马(P0.01)。2、低剂量细管子宫体输精技术和输精时间摸索研究。对97匹母马进行了在第一,二情期卵泡发育至直径达到≥30 mm时后,则使用低剂量细管子宫体输精法,每次平均输精量为2.4 ml,97匹母马中54匹受胎,妊娠率达到55.6%。3、母马早期妊娠诊断技术研究。配种后的14d左右,使用3.5/5.0 Hz的探头探测能到胚泡,并且检查到的图像画面清晰,具有较高的分辨率,即说明高频率探头适合做直肠探查来判断马匹的早期妊娠。
[Abstract]:10 horses with the same age and 6-8 years of age were selected, including 4 pure blood horses, 2 cloth hones, 4 Mongolia horses, and 97 empty mares for the low dose tube uterus for different estrus during different estrus and a series of pregnancy diagnosis by a portable B-mode ultrasonography after 14 days of insemination. Artificial insemination experiments have been made to obtain the data of the semen quality of three different species of male horse and the best period of mother horse insemination and the technique of using B ultrasonic instrument to diagnose the early pregnancy technique of the mother horse, and use these methods and data scientifically to promote the pastoral area according to local conditions, and improve the use of artificial insemination technology in pastoral areas to improve the horse. The specific results obtained by this experiment are as follows: 1, in the comparative study of the semen quality detection of different varieties of male horses, the average precision of the semen is significantly higher than the pure blood horse, Mongolia horse (P0.01) from the point of ejaculation, and the average semen density of the pure blood horse is significantly higher than that in the semen density in terms of the density of semen. Nyma, Mongolia horse (P0.01); from the total sperm count, the most common horse in the bud was the Mongolia horse, followed by the Mongolia horse. From the fresh sperm vitality, the vitality of the Mongolia semen was significantly higher than the pure blood horse, Bbu Joan Nima (P0.01).2, the low dose tube uterine body deferring technology and the spermatodefering study. The 97 mare were in the first, second estrous follicle. When the diameter reached more than 30 mm, the low dose tube uterine body insemination was used, the average amount of sperm delivery was 2.4 ml, and 54 of the 97 mare horses were pregnant. The pregnancy rate reached 55.6%.3, the early pregnancy diagnosis technique of the mare was studied. The 3.5/5.0 Hz probe was used to detect the blastocyst with the 3.5/5.0 Hz probe. It has high resolution, which means that high frequency probe is suitable for rectal exploration to determine the early pregnancy of horses.
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S821

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