饲粮中短期内单独及混合添加高水平燕麦β-葡聚糖和微晶纤维素对小鼠生长性能、器官指数和粪便细菌群落结构的影响

发布时间：2018-07-05 15:39

本文选题：燕麦β-葡聚糖 + 微晶纤维素　；参考：《动物营养学报》2017年07期

【摘要】：本试验旨在研究饲粮中短期内单独及混合添加高水平燕麦β-葡聚糖和微晶纤维素(MCC)对小鼠生长性能、器官指数、粪便细菌群落结构的影响。选取36只健康的体重为(17.95±0.95)g的BALB/c小鼠,按体重随机分为4组:对照组(CON组),饲粮不含燕麦β-葡聚糖和微晶纤维素;葡聚糖组(G组),饲粮含28%燕麦β-葡聚糖;MCC组(M组),饲粮含20%M CC;混合组(GM组),饲粮含14%燕麦β-葡聚糖和10%M CC。试验期为21 d。结果显示:1)各组小鼠全期(第1~21天)平均日增重(ADG)差异不显著(P0.05),而全期平均日采食量(ADFI)则差异显著(P0.05),表现为G组M组GM组CON组。2)各组小鼠脾脏指数差异不显著(P0.05),各纤维添加组(G组、M组、GM组)与CON组小鼠附睾脂肪垫指数差异不显著(P0.05),但G组和M组小鼠附睾脂肪垫指数均显著低于GM组(P0.05)。3)试验第4天和第7天,G组小鼠粪便细菌香农-威纳指数显著低于CON组(P0.05)。聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)图谱聚类分析显示,试验第13天、第17天时,各组小鼠粪便细菌群落结构差异明显,各组样品在进化树上各自聚类。由此可见,在小鼠饲粮中短期单独或混合添加高水平燕麦β-葡聚糖和MCC均可降低小鼠的ADFI,但不影响小鼠的ADG和脾脏指数;燕麦β-葡聚糖和MCC混合添加比单独添加更能促进小鼠附睾脂肪的沉积;高水平燕麦β-葡聚糖可降低小鼠粪便细菌的多样性;燕麦β-葡聚糖和MCC的添加均可改变小鼠粪便微生物区系,暗示小鼠后肠可能存在特异性利用这2种纤维的核心菌群。
[Abstract]:The purpose of this study was to study the effects of high level of oat 尾 -glucan and microcrystalline cellulose (MCC) on growth performance, organ index and fecal bacterial community structure in mice. Thirty-six healthy BALB / c mice weighing (17.95 卤0.95) g were randomly divided into four groups according to their body weight: control group (Con group), diet without 尾 -glucan and microcrystalline cellulose; Glucose group (G group), diet containing 28% oat 尾 -glucan MCC group (M group), diet containing 20 MCCs, mixed group (GM group), diet containing 14% oat 尾 -glucan and 10 MCCs. The experimental period is 21 days. The results showed that the average daily gain (ADG) of each group was not significantly different (P0.05), but the average daily intake (ADFI) was significantly different (P0.05), showing that there was no significant difference in spleen index in group M (group M) and group C (group C) (P0.05). There was no significant difference in epididymal fat pad index between group G and group C (P0.05), but the fat pad index of group G and M was significantly lower than that of group GM (P0.05) on the 4th and 7th day of the experiment. The Shannon-Weiner index of bacteria was significantly lower than that of Con group (P 0.05). Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) cluster analysis showed that, on the 13th and 17th day of the experiment, the fecal bacterial community structure of each group was significantly different, and the samples of each group were clustered in the evolutionary tree. It can be seen that supplementation of high level oat 尾 -glucan and MCC in diet alone or in combination can decrease ADFI in mice, but it does not affect ADG and spleen index of mice. The mixed addition of oat 尾 -glucan and MCC could promote the accumulation of epididymal fat in mice, and the high level of oat 尾 -glucan could reduce the diversity of fecal bacteria in mice. The addition of oat 尾 -glucan and MCC could change the faecal microflora of mice, suggesting that there might be a core flora of the two fibers in the mouse hindgut.
【作者单位】：四川农业大学动物营养研究所教育部动物抗病营养重点实验室;
【基金】：国家自然科学基金项目(31301987)
【分类号】：S816

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