复方天然植物制剂对奶牛回乳效果及调控激素的影响

发布时间：2018-07-07 08:16

  本文选题：奶牛 + 回乳　； 参考：《中国农业科学》2017年18期

【摘要】：【目的】探究复方天然植物制剂对奶牛回乳效果及回乳期奶牛血清胰岛素(INS)、氢化可的松(HC)、生长激素(GH)和胰岛素生长因子1(IGF-1)含量的影响。【方法】试验于2016年1—6月进行,选择四川省某规模化奶牛场半封闭统一舍饲,体重(582±41)kg、第2—4胎中国荷斯坦奶牛150头。从中选取体况良好,健康,乳房、乳汁均正常,即将进入回乳期,产奶量为(15.42±0.71)kg的妊娠后期即将干乳的健康奶牛80头。随机分为A、B、C、D组,每组20头,均采用逐渐干奶法回乳,回乳开始当天记为第0天,A组为对照组,不饲喂复方天然植物制剂。B、C、D组均从回乳开始第1天,每日上午8:00分别饲喂复方天然植物制剂400、500、600g,回乳完成后停止饲喂复方天然植物制剂。复方天然植物制剂由麦芽,朴硝,升麻,柴胡,香附,薏仁,蚕蜕,白术,黄芩,知母,苏梗,芡实,五味子,蒲公英,甘草等按一定比例组成。分别采集奶牛回乳期第0、1、3、5、7、9和11天尾静脉血,置于未加抗凝剂的离心管中,室温下静置1h,置离心机352×g离心10min,转移上层血清于EP管中,-20℃冻存。采用双抗体夹心酶联免疫吸附技术(ELISA)法检测血清INS、HC、GH和IGF-1含量,并记录回乳期奶牛单日产奶量。【结果】A、B、C、D 4组奶牛回乳时间分别为11、7、5、5天。各组回乳期奶牛血清IGF-1和HC含量均呈下降趋势,且对照组下降趋势较各复方天然植物制剂组慢,复方天然植物制剂组血清IGF-1、HC和GH含量在回乳期后回升,对照组血清INS含量上升趋势较各复方天然植物制剂组慢,第0至1天,各组奶牛血清HC、IGF-1和GH含量差异均不显著(P0.05);第3—7天,A组奶牛血清HC、GH和IGF-1含量极显著高于B、C、D组(P0.01),而血清INS含量极显著低于B、C、D组(P0.01);第9天,A、D组奶牛血清HC含量均显著高于C组(P0.05),A、B组奶牛血清GH和IGF-1含量均极显著低于C、D组(P0.01),A组奶牛血清INS含量极显著低于B、C、D组(P0.01);第11天,A组奶牛血清HC、GH和IGF-1含量极显著低于B、C、D组(P0.01),A组奶牛血清INS显著低于D组(P0.05),但极显著低于B、C组(P0.01);C、D组奶牛血清IGF-1、GH、HC和INS含量在整个回乳期均差异性不显著(P0.05)。在奶牛回乳期,产奶量、HC、GH和IGF-1两两间均呈极显著正相关(P0.01),INS与产奶量、HC、GH和IGF-1均呈极显著负相关(P0.01)。【结论】复方天然植物制剂能显著促进回乳期奶牛回乳,饲喂500g/日复方天然植物制剂,奶牛回乳时间为5d,回乳效果即可达到最佳。回乳期奶牛血清INS、HC、IGF-1含量和产奶量变化两两间均呈极显著相关,IGF-1、GH和HC对奶牛回乳起负向调控功能,而INS对奶牛回乳起正调控作用。
[Abstract]:[objective] to investigate the effects of compound natural plant preparation on the remilk effect and the contents of insulin (ins), hydrocortisone (HC), growth hormone (GH) and insulin growth factor 1 (IGF-1) in the serum of dairy cows during the remilk period. [methods] the experiment was conducted from January to June 2016. A large scale dairy farm in Sichuan Province was selected. The body weight was (582 卤41) kg and 150 Chinese Holstein cows were born 2-4. 80 healthy cows with good body condition, healthy breast, normal breast and milk were selected, and the milk yield was (15.42 卤0.71) kg in the second trimester. The rats were randomly divided into two groups: group A (n = 20) and group A (n = 20). Each group (n = 20) was relaced with gradual dry milk, and the group A was recorded as the control group on day 0, and the control group was not fed with compound natural botanical preparation. Each day at 8:00, the compound natural plant preparations were fed with 400500, 600 g, and stopped feeding the compound natural plant preparations after the milk was finished. Compound natural plant preparations are made up of malt, Park nitrite, cohosh, Bupleurum, Apleurum, Coylea, silkworm slough, Atractylodes macrocephala, Scutellaria baicalensis, Anemarrhena sativa, Euryale seed, Schisandra chinensis, Taraxacum dandelion, Glycyrrhiza. The venous blood samples were collected from the tail vein of the cows on day 0, 1, 3, 5, 5, 7, 9 and 11, respectively. The venous blood was placed in a centrifuge tube without anticoagulant for 1 h at room temperature, then centrifuged with 352 脳 g centrifuge for 10 min. The upper serum was frozen in EP tube at -20 鈩，

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