氟尼辛葡甲胺缓解奶牛分娩应激的疗效观察

发布时间：2018-07-09 13:11

本文选题：奶牛 + 分娩应激　；参考：《中国农业大学》2017年博士论文

【摘要】：分娩应激是临产母牛在分娩过程中发生的生理和行为上的特异性或非特异性反应。分娩是伴随着急性疼痛的痛苦过程。分娩启动后,有规律的子宫收缩引起阵痛,机体在此疼痛的刺激下产生应激反应。分娩应激是奶牛产后诸多疾病的诱发因素,但临床实践中关于奶牛分娩过程中疼痛和应激的报道较少。本研究试图阐明产后注射镇痛药-氟尼辛葡甲胺(flunixinmeglumine,FM)缓解奶牛疼痛性分娩应激的有效性与可行性。为了探讨分娩过程中存在分娩应激,本研究选取临床健康,体况正常,2～5胎次,自然分娩,产后21d无疾病的经产奶牛5头,分别于分娩前后采集尾静脉血,提取RNA,进行高通量转录组测序(RNA-seq),获得分娩前后差异表达基因,并用qPCR进行验证。结果表明与应激相关的HSP90α和PI3Kr2基因表达出现上调,说明分娩过程中发生了应激反应。为了进一步验证分娩应激的存在,本研究分别选取了临床健康,体况正常,2～5胎次,自然分娩,产后21d无疾病的经产奶牛10头,分别于分娩前7d至分娩后7d每日采集尾静脉血,检测血清中 Cortisol、MDA、TAOC、T-SOD、HSP90 和 HSP72 以及微量元素 Zn、Cu、Se 和 Mg水平。结果表明奶牛分娩后血清MDA、HSP90、TAOC和T-SOD水平显著升高(P0.05),微量元素Zn和Mg含量显著降低(P0.05),进一步证实了分娩过程中存在分娩应激,而且发生了氧化应激损伤。为了阐明疼痛是诱发分娩应激的重要因素,本研究进行了产后镇痛试验。选取临床健康,体况正常,2～5胎次,自然分娩,产后无疾病发生的经产奶牛20头,随机分为对照组和试验组(产后注射FM, 2mg/kg,1次/d,连用3d),分别于产后1～4d采集尾静脉血,检测血清Cortisol、MDA、HSP90、TAOC和T-SOD以及微量元素Zn、Cu、Se和Mg的含量。结果表明产后注射FM可降低血清Cortisol、MDA、Zn、Cu、Se和Mg的水平,增加血清TAOC和T-SOD的水平。说明疼痛是分娩应激的诱发因素,产后镇痛可以缓解分娩应激,增强机体的抗氧化能力。为了阐述产后注射FM对奶牛血液生化指标和生产性能的影响,本研究选取临床健康,体况正常,2～5胎次,自然分娩的经产奶牛50头,随机分为对照组和试验组(产后注射FM, 2mg/kg,1次/d,连用3d),于产后7d采集尾静脉血,检测血液钙、磷、糖、β-羟丁酸的含量,产后20d采集乳样,检测乳中体细胞数和乳质成分并记录每日产奶量及奶牛产后发病情况。结果表明产后注射FM可显著改善奶牛产后低血糖(P 0.01),使21日平均产奶量提高2.40kg (P0.05), 90日平均产奶量提高2.19kg (P0.05),奶牛乳房炎的发病率降低8% (P0.05)。为了进一步探讨FM对奶牛产后疾病发生率的影响,本试验对某奶牛场用药前一年和用药后一年后的奶牛乳房炎、真胃变位、蹄病、酮病、腹泻及子宫内膜炎发生率进行了统计。得知该场应用FM后奶牛乳房炎年发病率由41.87%降为35.22%(P0.01),真胃变位的年发病率由10.42%降为8.19% (P0.05),蹄病的年发病率由27.10%降为22.45% (P0.05)。综上所述,奶牛分娩过程中存在应激反应,分娩过程中的疼痛是引起应激反应的重要原因,产后注射FM可有效缓解分娩应激,并能够降低奶牛乳房炎、真胃变位和蹄病的发病率。
[Abstract]:Labor stress is a specific or nonspecific reaction in the process of birth of a cow during childbirth. Delivery is a painful process accompanied by acute pain. After birth, a regular contraction of the uterus causes pain, and the body produces stress responses under the pain of the body. Delivery stress is the lure of many diseases after the cow. In clinical practice, there are few reports of pain and stress in the process of dairy delivery. This study attempts to elucidate the effectiveness and feasibility of postpartum injection of flunixinmeglumine (FM) in relieving the pain of dairy cows. In order to explore the existence of labor stress during delivery, this study selects clinical health. Healthy, 2~5 parity, 2~5 parity, natural childbirth, 5 postpartum parturition cows with no disease, collect tail vein blood before and after childbirth, extract RNA, carry out high flux transcriptional sequence (RNA-seq), obtain differential expression genes before and after delivery, and verify with qPCR. The results show that the expression of HSP90 alpha and PI3Kr2 related to stress appears to appear. In order to further verify the existence of labor stress, in order to further verify the existence of labor stress, this study selected clinical health, normal body condition, 2~5 parity, natural childbirth, and postpartum 21d without disease of 10 cows, respectively, from 7d to 7d after childbirth to collect tail vein blood each day, and to detect Cortisol, MDA, TAOC, T- in serum. SOD, HSP90 and HSP72, and trace elements Zn, Cu, Se and Mg levels. The results showed that serum MDA, HSP90, TAOC and T-SOD levels increased significantly after childbirth (P0.05). An important factor of delivery stress was carried out, and the postpartum analgesia test was carried out in this study. The clinical health, normal body condition, 2~5 parity, natural childbirth, and 20 postpartum parturition cows were randomly divided into the control group and the experimental group (FM, 2mg/kg, 1 /d, and 3D), and the tail vein blood was collected from 1 to 4D after postpartum, and the serum Cortis was detected respectively. Ol, MDA, HSP90, TAOC and T-SOD as well as the content of Zn, Cu, Se and Mg. The results show that postpartum injection of FM can reduce the level of serum Cortisol, MDA, etc. The effect of postpartum injection of FM on blood biochemical indexes and production performance of dairy cows was studied. This study selected clinical health, normal body condition, 2~5 parity and 50 natural childbirth cows. They were randomly divided into control group and experimental group (FM, 2mg/kg, 1 /d, 3D). After postpartum 7d, caudal vein blood was collected, and blood calcium, phosphorus, sugar, beta hydroxybutyric acid was detected. 20d milk samples were collected after postpartum, the number of somatic cells and milk components in milk were detected and milk production was recorded daily and the postpartum incidence of dairy cows was recorded. The results showed that postpartum injection of FM could significantly improve postpartum hypoglycemia (P 0.01), increase the average milk production of 21 days, increase 2.40kg (P0.05), increase the average milk production on 90 days, and increase the milk of dairy cow mastitis. The incidence of disease was reduced by 8% (P0.05). In order to further investigate the effect of FM on the incidence of postpartum diseases in dairy cows, this experiment was conducted to calculate the incidence of Dairy Mastitis, real stomach change, hoofed disease, ketosis, diarrhea and endometritis after one year of drug use and one year after medication in a dairy farm. It was found that the annual incidence of dairy cow mastitis after FM was 41.87. % decreased to 35.22% (P0.01), the annual incidence of true gastric displacement decreased from 10.42% to 8.19% (P0.05), and the annual incidence of hoof disease was reduced from 27.10% to 22.45% (P0.05). In summary, there was stress reaction in the process of childbirth, and pain during childbirth was an important cause of stress response. Postpartum injection of FM could effectively alleviate labor stress and be reduced. The incidence of cow mastitis, true stomach change and hoof disease.
【学位授予单位】：中国农业大学
【学位级别】：博士
【学位授予年份】：2017
【分类号】：S858.23

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