温肾益精散对肾阳虚模型大鼠生长性能及免疫功能影响的研究

发布时间：2018-07-09 23:48

本文选题：温肾益精散 + 肾阳虚　；参考：《内蒙古农业大学》2017年硕士论文

【摘要】：生长迟缓是家畜临床最常见病证之一,中兽医学认为生长迟缓与肾阳虚关系密切。本试验采用氢化可的松大鼠后肢肌内注射构建大鼠肾阳虚模型以研究温肾益精散对其生长性能及免疫功能的影响。试验将SD大鼠随机分为空白对照组、模型组、金匮肾气汤组、温肾益精散高、中和低剂量组。记录各组大鼠体重和采食量变化,计算大鼠饲料转化率,并进行血液相关指标测定、免疫器官组织形态学观察,以及肝脏RNA相关基因表达的检测。结果显示:1.肾阳虚造模可显著降低大鼠体重增加速度(P0.05);显著降低大鼠体温(P0.05);显著降低大鼠免疫器官脏器指数(P0.05);显著降低大鼠血清中T3、T4、E2、T、ACTH、CORT 和GH含量(P0.05)。2.温肾益精散中、低剂量组和金匮肾气汤组可显著提高模型大鼠体重增加速度(P0.05);显著提高模型大鼠平均日增重(P0.05);各给药组均可提高模型大鼠饲料转化率。3.温肾益精散中、低剂量组和金匮肾气汤组可显著降低模型大鼠血清中SST含量(P0.05);显著提高模型大鼠血清中IGF-1含量(P0.05);温肾益精散高剂量组可显著提高模型大鼠血清中IGF-1含量(P0.05);温肾益精散中剂量组和金匮肾气汤组可显著提高模型大鼠肝脏中JAK2和IGF-1mRNA表达量(P0.05)。4.温肾益精散中、低剂量组和金匮肾气汤组可显著提高模型大鼠胸腺和脾脏脏器指数(P0.05);温肾益精散高剂量组可显著提高模型大鼠胸腺脏器指数(P0.05);温肾益精散中剂量组和金匮肾气汤组可显著提高模型大鼠血清中IgA、IgG、IgM和IL-2、IL-4含量(P0.05);温肾益精散低剂量组可显著提高模型大鼠血清中IL-2和IL-4含量(P0.05);各给药组均可改善模型大鼠胸腺和脾脏组织形态变化。结论:1.温肾益精散可改善肾阳虚模型大鼠生长性能,提高肾阳虚模型大鼠生长速度。2.温肾益精散可提高肾阳虚模型大鼠免疫功能。3.温肾益精散药理效果以中剂量组(1g/mL)为佳。
[Abstract]:Growth retardation is one of the most common syndromes in animal clinic. Chinese veterinary medicine thinks that growth retardation is closely related to deficiency of kidney yang. A rat model of kidney yang deficiency was established by intramuscular injection of hydrocortisone into hindlimb to study the effect of Wenshen Yijing Powder on its growth performance and immune function. Sprague-Dawley rats were randomly divided into blank control group, model group, Jinkui Shenqi decoction group, Wenshen Yijing San high, medium and low dose groups. The changes of body weight and feed intake were recorded, feed conversion rate was calculated, blood related indexes, morphological observation of immune organs and expression of RNA related genes in liver were measured. The result is 1: 1. Kidney Yang deficiency model can significantly reduce the rate of weight gain in rats (P0.05); significantly decreased the body temperature (P0.05); significantly decreased the organ index of immune organs (P0.05); significantly decreased the serum T3T3OT4E2ACTHCORT and GH content (P0.05). 2. Wenshen Yijing Powder, low dose group and Jingui Shenqi decoction group can significantly increase the weight gain rate of model rats (P0.05); significantly increased the average daily gain of model rats (P0.05); each drug group can increase the feed conversion rate of model rats. 3. Wenshen Yijing Powder, Low dose group and Jinkui Shenqi decoction group can significantly reduce the SST content in the model rat serum (P0.05), significantly increase the IGF-1 content in the model rat serum (P0.05), WYJS high dose group can significantly increase the IGF-1 content in the model rat serum (P0.05); Wenshen Yi-qi decoction group can significantly increase the IGF-1 content in the serum of the model rats (P0.05); Jisan medium dose group and Jinkui Shenqi decoction group could significantly increase the expression of JAK2 and IGF-1 mRNA in the liver of model rats (P0.05). Wenshen Yijing Powder, Low dose group and Jinkui Shenqi decoction group could significantly increase the thymus and spleen organ index (P0.05), WYJS high dose group could significantly increase the thymus organ index (P0.05), WYJS medium dose group and Jinshen Qi decoction group could significantly increase the thymus organ index (P0.05), and Wenshen Yijing Powder moderate dose group and Jinshenqi decoction group could significantly increase the thymus organ index (P0.05). The serum levels of IgAGN IgM and IL-2 IL-4 in model rats were significantly increased (P0.05), IL-2 and IL-4 in serum of model rats were significantly increased in low dose group (P0.05), and the morphologic changes of thymus and spleen of model rats were improved in each administration group. Conclusion 1. Wenshen Yijing Powder could improve the growth performance and increase the growth rate of kidney yang deficiency rats. Wenshen Yijing Powder can improve the immune function of rats with deficiency of kidney yang. The pharmacological effect of Wenshen Yijing Powder was better in the middle dose group (1 g / mL).
【学位授予单位】：内蒙古农业大学
【学位级别】：硕士
【学位授予年份】：2017
【分类号】：S853.74

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