益生菌对雏鸡免疫器官免疫功能及β-防御素-1mRNA表达的影响
发布时间：2018-07-13 18:46
【摘要】：以1日龄雏鸡为研究对象,应用荧光定量PCR等技术,结合器官组织石蜡切片及组织化学染色等方法,通过免疫器官——胸腺、脾脏、法氏囊鸡β-防御素-1(Gal-1)mRNA表达,浆细胞数量及其核浆比,TANAE+细胞数量等有关指标动态变化的检测,较全面系统的研究了不同种类益生菌对雏鸡免疫器官免疫功能和Gal-1mRNA表达的影响,旨在为益生菌及其制剂在动物生产中的应用及其研发新产品提供科学实验依据,研究结果发现:(1)雏鸡单独应用双歧杆菌后,胸腺、脾脏、法氏囊中TANAE+细胞数量均于7~13日龄时较对照雏鸡明显增多(P0.05);脾脏、法氏囊和胸腺中的浆细胞数量分别于10~13日龄和7~10日龄时较对照雏鸡明显增多(P0.05);但Gal-1mRNA表达于10~13日龄时较对照雏鸡明显提高(P0.05或P0.01)。(2)雏鸡单独应用乳酸杆菌后,脾脏和法氏囊中浆细胞和TANAE+细胞数量以及Gal-1m RNA均于7~13日龄时显著或极显著高于对照组雏鸡(P0.05或P0.01),而胸腺中浆细胞和TANAE+细胞数量于7~10日龄时明显多于对照组雏鸡(P0.05),但Gal-1mRNA表达于7日龄时较对照雏鸡明显提高(P0.05或P0.01)。(3)双歧杆菌和乳酸杆菌联合应用雏鸡后,胸腺、脾脏和法氏囊中的TANAE+细胞数量分别于7~10、13和10~13日龄时明显多于对照组雏鸡(P0.05);免疫器官中的浆细胞均与7日龄时明显多于对照组雏鸡(P0.05);但Gal-1mRNA表达于10~13日龄时较对照雏鸡明显提高(P0.05或P0.01)。乳酸杆菌对雏鸡免疫器官T淋巴细胞和浆细胞数量及Gal-1m RNA表达的作用效果优于双歧杆菌。
[Abstract]:The expression of 尾 -defensin-1 (Gal-1) mRNA in the immune organs of thymus, spleen and Fabricius chicks was studied by fluorescence quantitative PCR, paraffin section and histochemical staining. The effects of different probiotics on immune function of immune organs and expression of Gal-1 mRNA in chicks were studied comprehensively and systematically by detecting the dynamic changes of plasma cell number and the number of TANAE cells, such as the number of plasma cells and the number of TANAE cells. The aim of this study was to provide scientific experimental basis for the application of probiotics and their preparations in animal production and to develop new products. The results showed that: (1) the thymus and spleen of chicks were treated with bifidobacterium alone. The number of TANAE cells in the bursa of Fabricius was significantly higher than that of the control chicks at the age of 7 ~ 13 days (P0.05). The number of plasma cells in the bursa of Fabricius and thymus was significantly higher than that in the control group at the age of 10 and 10 days (P0.05), but the expression of Gal-1 mRNA in the thymus was significantly higher than that in the control (P0.05 or P0.01). (_ 2 alone), but the expression of Gal-1 mRNA in the thymus was significantly higher than that in the control (P0.05 or P0.01). (_ 2). The number of plasma cells and TANAE cells and Gal-1m RNA in spleen and bursa of Fabricius were significantly or extremely higher than those in the control group (P0.05 or P0.01), but the number of plasma cells and TANAE cells in thymus was significantly higher than that in the control group (P0.05). However, the expression of Gal-1 mRNA at 7 days old was significantly higher than that in the control (P0.05 or P0.01). (3) after the combination of Bifidobacterium and Lactobacillus. Thymus gland, The number of TANAE cells in spleen and bursa of Fabricius was significantly higher than that in the control group at the age of 7 days (P0.05), the plasma cells in the immune organs were significantly higher than those in the control group (P0.05), but the expression of Gal-1 mRNA in the spleen and bursa of Fabricius was significantly higher than that in the control group at the age of 1013 days (P0.05), but the expression of Gal-1 mRNA was higher than that in the control group at the age of 1013 days. Chicken significantly increased (P0.05 or P0.01). The effect of Lactobacillus on the number of T lymphocytes and plasma cells and the expression of Gal-1m RNA in chicken immune organs was better than that of Bifidobacterium.
【学位授予单位】：东北农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S831.5
本文编号：2120397
[Abstract]:The expression of 尾 -defensin-1 (Gal-1) mRNA in the immune organs of thymus, spleen and Fabricius chicks was studied by fluorescence quantitative PCR, paraffin section and histochemical staining. The effects of different probiotics on immune function of immune organs and expression of Gal-1 mRNA in chicks were studied comprehensively and systematically by detecting the dynamic changes of plasma cell number and the number of TANAE cells, such as the number of plasma cells and the number of TANAE cells. The aim of this study was to provide scientific experimental basis for the application of probiotics and their preparations in animal production and to develop new products. The results showed that: (1) the thymus and spleen of chicks were treated with bifidobacterium alone. The number of TANAE cells in the bursa of Fabricius was significantly higher than that of the control chicks at the age of 7 ~ 13 days (P0.05). The number of plasma cells in the bursa of Fabricius and thymus was significantly higher than that in the control group at the age of 10 and 10 days (P0.05), but the expression of Gal-1 mRNA in the thymus was significantly higher than that in the control (P0.05 or P0.01). (_ 2 alone), but the expression of Gal-1 mRNA in the thymus was significantly higher than that in the control (P0.05 or P0.01). (_ 2). The number of plasma cells and TANAE cells and Gal-1m RNA in spleen and bursa of Fabricius were significantly or extremely higher than those in the control group (P0.05 or P0.01), but the number of plasma cells and TANAE cells in thymus was significantly higher than that in the control group (P0.05). However, the expression of Gal-1 mRNA at 7 days old was significantly higher than that in the control (P0.05 or P0.01). (3) after the combination of Bifidobacterium and Lactobacillus. Thymus gland, The number of TANAE cells in spleen and bursa of Fabricius was significantly higher than that in the control group at the age of 7 days (P0.05), the plasma cells in the immune organs were significantly higher than those in the control group (P0.05), but the expression of Gal-1 mRNA in the spleen and bursa of Fabricius was significantly higher than that in the control group at the age of 1013 days (P0.05), but the expression of Gal-1 mRNA was higher than that in the control group at the age of 1013 days. Chicken significantly increased (P0.05 or P0.01). The effect of Lactobacillus on the number of T lymphocytes and plasma cells and the expression of Gal-1m RNA in chicken immune organs was better than that of Bifidobacterium.
【学位授予单位】：东北农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S831.5
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