妊娠后期宫内生长限制对蒙古绵羊胎儿胸腺细胞增殖影响及机理研究
发布时间：2018-07-13 21:39
【摘要】：本论文系统的阐述了妊娠后期IUGR对蒙古绵羊胎儿胸腺细胞增殖的相关因子表达的影响。选取24只经同期妊娠的健壮蒙古绵羊作为研究对象。妊娠期90 d时,挑取6只重量相近的母羊进行屠宰,余下18只绵羊根据重量随机分为3个不同营养水平处理组：0.18 MJME-BW-0.75·d-1 (RG1:rrn=6),0.33 MJME·BW0-0.75·d-1(RG2:n=6)和自由采食(CG：n=6),将剩余三个营养水平饲喂至妊娠期140 d,剩余各组6只妊娠羊进行屠宰试验。试验结果表明：(1)妊娠后期IUGR引起RG1组胎儿体重(p0.01)、胸腺重(p0.01)、总DNA含量(p0.01)、细胞增殖指数(p0.05)、PCNA个数(p0.01)极显著低于CG组,G0/G1期细胞比例明显高于CG组(p0.05)。RG2组胎儿体重(p0.01)胸腺重(p0.05)、总DNA含量(p0.05)显著低于CG组,IUGR胎儿胸腺的DNA合成受限制程度显著,是导致细胞增殖受限的主要原因之一。(2)RG1组GHR (p0.01)、IGF-1R (p0.01)、IGF-2R (p0.01)、CyclinB1 (p0.05)、CyclinD (p0.05)、CDK1 (p0.05)、CDK2 (p0.01)、CDK4 (p0.05)、 E2F1 (p0.01)、E2F4 (p0.01)、E2F5 (p0.01)基因表达量显著低于CG组,RG2组GHR (p0.01)、IGF-1R (p0.05)、CDK1 (p0.05)、CDK2 (p0.01)、CDK4 (p0.01)、E2F1 (p0.05)、E2F5 (p0.01)基因表达均显著低于CG组,IGF-2R、 CyclinB1、E2F4呈降低趋势,但差异均不显著(p0.05)。(3)RG1组GH (p0.01)、IGF-1 (p0.05)、IGF-2 (p0.01)、GHR(p0.05) IGF-1R (p0.05)、IGF-2R (p0.05)、CyclinA (p0.05)、CyclinB (p0.05) CyclinD (p0.05)、CyclinE (p0.05)、E2F2 (p0.05)均显著低于CG组,IGFBP-1显著高于CG组(p0.05),E2F1、E2F4、E2F5均呈增高趋势,但差异不显著(p0.05),CDK1、CDK2、CDK4呈降低趋势,但差异均不显著(p0.05)；RG2组IGFBP-2 (p0.01)、GHR (p0.05)显著低于CG组,IGFBP-1显著高于CG组(p0.05)。CyclinA、CyclinB、CyclinD、CyclinE、CDK1、CDK2、CDK4、E2F2蛋白水平表达呈降低趋势,但差异不显著(p0.05), E2F1、E2F4、E2F5均呈增高趋势,但差异均不显著(p0.05)。
[Abstract]:The effects of IUGR on the expression of factors related to the proliferation of fetal thymocytes in Mongolian sheep were systematically discussed in this paper. Twenty-four healthy Mongolian sheep with simultaneous pregnancy were selected as study objects. At 90 days gestation, 6 ewes of similar weight were selected and slaughtered. The remaining 18 sheep were randomly divided into three groups: 0.18 MJME-BW-0.75 d-1 (RG1: rrrnnf6) 0.33 MJME BW0-0.75 d-1 (RG2: nrnf6) and CG2: nong6 according to their weight. The remaining three nutritional levels were fed to 140 days of gestation, and six sheep in each group were slaughtered. The results showed that: (1) the fetal weight (p0.01), thymus weight (p0.01), total DNA content (p0.01), proliferative index (p0.05) PCNA number (p0.01) of IUGR induced by IUGR in RG1 group were significantly lower than those in CG group (p0.05) 路RG2 group (p0.01). (p0.05), total DNA content (p0.05) was significantly lower than that in CG group. 鏄鑷寸粏鑳炲娈栧彈闄愮殑涓昏鍘熷洜涔嬩竴.(2)RG1缁凣HR (p0.01),IGF-1R (p0.01),IGF-2R (p0.01),CyclinB1 (p0.05),CyclinD (p0.05),CDK1 (p0.05),CDK2 (p0.01),CDK4 (p0.05), E2F1 (p0.01),E2F4 (p0.01),E2F5 (p0.01)鍩哄洜琛ㄨ揪閲忔樉钁椾綆浜嶤G缁，
本文编号：2120818
[Abstract]:The effects of IUGR on the expression of factors related to the proliferation of fetal thymocytes in Mongolian sheep were systematically discussed in this paper. Twenty-four healthy Mongolian sheep with simultaneous pregnancy were selected as study objects. At 90 days gestation, 6 ewes of similar weight were selected and slaughtered. The remaining 18 sheep were randomly divided into three groups: 0.18 MJME-BW-0.75 d-1 (RG1: rrrnnf6) 0.33 MJME BW0-0.75 d-1 (RG2: nrnf6) and CG2: nong6 according to their weight. The remaining three nutritional levels were fed to 140 days of gestation, and six sheep in each group were slaughtered. The results showed that: (1) the fetal weight (p0.01), thymus weight (p0.01), total DNA content (p0.01), proliferative index (p0.05) PCNA number (p0.01) of IUGR induced by IUGR in RG1 group were significantly lower than those in CG group (p0.05) 路RG2 group (p0.01). (p0.05), total DNA content (p0.05) was significantly lower than that in CG group. 鏄鑷寸粏鑳炲娈栧彈闄愮殑涓昏鍘熷洜涔嬩竴.(2)RG1缁凣HR (p0.01),IGF-1R (p0.01),IGF-2R (p0.01),CyclinB1 (p0.05),CyclinD (p0.05),CDK1 (p0.05),CDK2 (p0.01),CDK4 (p0.05), E2F1 (p0.01),E2F4 (p0.01),E2F5 (p0.01)鍩哄洜琛ㄨ揪閲忔樉钁椾綆浜嶤G缁，
本文编号：2120818
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