奶牛酮病检测方法的比较和牛乳、血清中β-羟丁酸相关性研究

发布时间：2018-07-14 17:47

【摘要】：奶牛酮病是常见的由碳水化合物和脂肪代谢紊乱引起的一种群发性代谢病,该病主要以酮血症、酮尿症、酮乳症为特征,常表现为低血糖症,血液中游离脂肪酸升高,脂肪肝,糖原水平下降等,多发于高产奶牛中。酮病虽然能够治愈,也很少引起奶牛死亡,但酮病会造成产奶量下降、乳质量和繁殖力降低,继发生殖系统疾病和内分泌紊乱等多种疾病,增加了治疗费用,给奶牛养殖业造成较大损失。及时有效地预防和治疗奶牛酮病对提高奶牛养殖业发展水平具有重要意义。目的:本研究以石河子地区规模化奶牛场96头奶牛牛乳和血清为材料,测定牛乳和血清中酮体、β-羟丁酸浓度,旨在研究适合大型养殖场奶牛酮病监测方法和酮病奶牛牛乳和血清中β-羟丁酸(BHBA)浓度的相关性。方法:1、酮粉法、试剂法、ROSS法、Rothera法等四种方法定性检测牛乳;改良式水杨醛法定量检测奶牛血清,定义血清中酮体含量超过100mg/L为酮病奶牛。2、应用磷钨酸沉淀法对酮病奶牛牛乳脱蛋白处理后采用酶荧光法测定牛乳中β-羟丁酸浓度,应用酶联免疫分析法测定酮病奶牛血清中β-羟丁酸浓度,用SPSS10.0进行分析,相关系数pearson。结果:1、改良式水杨醛法测得96头奶牛患酮病数量为40头,四种定性检测牛乳方法中酮粉法准确率最高,符合率达85.42%。2、酮病奶牛乳液中BHBA平均浓度为1.21±0.25mmol/L,血清中BHBA平均浓度为2.19±0.35mmol/L,随着胎次的增加,牛乳中的BHBA浓度也逐渐升高,血清中BHBA浓度变化不显著,其二者的相关系数较高;产奶量越高,牛乳和血清中的BHBA含量也在逐渐增高,且二者的相关系数较高。结论:1、酮粉法检测酮病奶牛易操作检测效率高,假阳性率低,较适合作为大型养殖场酮病监测方法。2、在酮病奶牛中牛乳与血清中β-羟丁酸相关性为0.8102-0.9105,因此可通过牛乳中β-羟丁酸检测来判定是否为酮病奶牛,作为奶牛酮病早期诊断依据。
[Abstract]:Ketosis is a common metabolic disorder caused by carbohydrate and fat metabolism in dairy cows. It is characterized by ketonemia, ketouria and ketone lactation. It is characterized by hypoglycemia, increased free fatty acids in blood and fatty liver. Glycogen level decreased, mainly in high-yielding cows. Although ketosis can be cured and rarely causes cows to die, ketosis can lead to a decrease in milk production, a decrease in milk quality and reproduction, secondary diseases such as reproductive system diseases and endocrine disorders, which increase the cost of treatment. To the dairy cattle industry caused a large loss. Timely and effective prevention and treatment of ketosis in dairy cattle is of great significance to improve the development level of dairy cattle breeding industry. Objective: to determine the concentration of ketone and 尾 -hydroxybutyric acid in milk and serum of 96 dairy cows in Shihezi area. The aim of this study was to study the relationship between ketosis monitoring methods and 尾 -hydroxybutyric acid (BHBA) concentrations in milk and serum of dairy cows. Methods the milk was detected qualitatively by the methods of 1: 1, ketone powder, ross and Rothera, and the serum of cows was detected by modified salicylaldehyde method. The ketone body content in serum was defined as ketosis cow. The 尾 -hydroxybutyric acid concentration in milk was determined by enzyme fluorescence method after deproteinization of ketosis cow milk by phosphotungstic acid precipitation method. Serum 尾 -hydroxybutyric acid (尾 -hydroxybutyric acid) concentration in ketosis cows was determined by enzyme-linked immunosorbent assay (Elisa) and analyzed by SPSS 10.0. The correlation coefficient was Pearson. Results by using the modified salicylaldehyde method, 40 cows were found to suffer from ketosis, and the accuracy of ketone powder method was the highest among the four qualitative methods. The coincidence rate was 85.42 路2.The average concentration of BHBA in milk of ketosis cow was 1.21 卤0.25 mmol / L, and the average concentration of BHBA in serum was 2.19 卤0.35 mmol / L. The concentration of BHBA in milk gradually increased with the increase of birth number, but the concentration of BHBA in serum did not change significantly, and the correlation coefficient between BHBA and BHBA was higher. The higher the milk yield, the higher the BHBA content in milk and serum, and the correlation coefficient was higher. Conclusion the detection efficiency and false positive rate of ketone powder method are high and false positive rate is low. The correlation between milk and serum 尾 -hydroxybutyric acid is 0.8102-0.9105, so it can be determined whether it is ketosis cow or not by detecting 尾 -hydroxybutyric acid in milk, which can be used as the basis for early diagnosis of ketosis in dairy cows.
【学位授予单位】：石河子大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.23

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