添加通路阻断剂对IGF-1影响奶牛乳腺上皮细胞增殖与凋亡的研究
发布时间：2018-07-15 17:04
【摘要】：试验旨在研究胰岛素样生长因子-1(insulin-like growth factor 1,IGF-1)对奶牛乳腺上皮细胞(bovine mammary epithelial cells,BMECs)增殖的影响,为后期利用IGF-1调控乳腺发育奠定理论基础。以奶牛乳腺上皮细胞为材料,首先分4组分别外源添加0(对照组)、10、50、100μg/mL IGF-1且分别培养12、24、48、72h,测定抑制BMECs凋亡率的最佳浓度;然后分6组:单纯BMECs组、BMECs+IGF-1组、BMECs+LY294002组、BMECs+IGF-1+LY294002组、BMECs+RAPA组和BMECs+IGF-1+RAPA组,采用流式细胞术测定各组细胞凋亡率。结果表明,外源性添加IGF-1对BMECs凋亡率具有抑制作用,最佳浓度为50μg/mL;BMECs+IGF-1+LY294002与BMECs+IGF-1+RAPA组细胞凋亡率均极显著高于BMECs+IGF-1组(P0.01)。推断IGF-1能够活化PI3K/Akt/mTOR信号通路,参与BMECs凋亡的调控作用,进而抑制BMECs细胞凋亡;IGF-1可能会对被抑制PI3K/Akt/mTOR信号通路产生"修复"机制,使其能够重新参与BMECs的生命进程。
[Abstract]:The aim of the experiment was to study the effect of insulin-like growth factor 1 IGF-1 on the proliferation of bovine breast epithelial (bovine mammary epithelial cells BMECs, and to lay a theoretical foundation for the regulation of breast development by IGF-1. Dairy mammary epithelial cells were first divided into 4 groups: 10 050 渭 g / mL IGF-1 supplemented with 0 (control) 1050g / mL IGF-1 and cultured for 72 h, respectively. The optimal concentration of inhibiting apoptosis rate of BMECs was determined. Then it was divided into six groups: BMECs IGF-1 group, BMECs LY294002 group, BMECs IGF-1 LY294002 group and BMECs IGF-1 RAPA group. The results showed that exogenous addition of IGF-1 inhibited the apoptosis rate of BMECs. The best concentration of IGF-1 IGF-1 LY294002 and BMECs IGF-1 RAPA group was significantly higher than that of BMECs IGF-1 group (P0.01). It is inferred that IGF-1 can activate the PI3K / Akt / mTOR signaling pathway, participate in the regulation of BMECs apoptosis, and then inhibit the apoptosis of BMECs cells. IGF-1 may produce a "repair" mechanism on the inhibited PI3K / Akt / mTOR signaling pathway and enable IGF-1 to re-participate in the life process of BMECs.
【作者单位】： 新疆农业大学动物科学学院;
【基金】：国家自然科学基金(31560645) 新疆维吾尔自治区重点实验室开放课题(2015KL018) 中国博士后基金委资助
【分类号】：S823
[Abstract]:The aim of the experiment was to study the effect of insulin-like growth factor 1 IGF-1 on the proliferation of bovine breast epithelial (bovine mammary epithelial cells BMECs, and to lay a theoretical foundation for the regulation of breast development by IGF-1. Dairy mammary epithelial cells were first divided into 4 groups: 10 050 渭 g / mL IGF-1 supplemented with 0 (control) 1050g / mL IGF-1 and cultured for 72 h, respectively. The optimal concentration of inhibiting apoptosis rate of BMECs was determined. Then it was divided into six groups: BMECs IGF-1 group, BMECs LY294002 group, BMECs IGF-1 LY294002 group and BMECs IGF-1 RAPA group. The results showed that exogenous addition of IGF-1 inhibited the apoptosis rate of BMECs. The best concentration of IGF-1 IGF-1 LY294002 and BMECs IGF-1 RAPA group was significantly higher than that of BMECs IGF-1 group (P0.01). It is inferred that IGF-1 can activate the PI3K / Akt / mTOR signaling pathway, participate in the regulation of BMECs apoptosis, and then inhibit the apoptosis of BMECs cells. IGF-1 may produce a "repair" mechanism on the inhibited PI3K / Akt / mTOR signaling pathway and enable IGF-1 to re-participate in the life process of BMECs.
【作者单位】： 新疆农业大学动物科学学院;
【基金】：国家自然科学基金(31560645) 新疆维吾尔自治区重点实验室开放课题(2015KL018) 中国博士后基金委资助
【分类号】：S823
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