一株奶山羊伪结核棒状杆菌的分离与鉴定

发布时间：2018-07-16 08:54

【摘要】：为获得陕西某羊场病羊脓包中的病原菌,以无菌采集的病羊颈部脓汁为材料,进行细菌分离培养,鉴定分离菌株的形态特征、培养特性、生化特性,挑取分离菌株进行协同溶血试验(cAMP)和16SrRNA基因的PCR扩增,并对序列进行分析,NJ法构建系统发育树;用分离菌株分别接种Balb/c小鼠和奶山羊;药敏试验鉴定分离株的耐药性。结果显示,分离菌株的形态特征、培养特性、生化特性与伯杰细菌鉴定手册所述伪结核棒状杆菌特性一致;测序显示分离菌株与GenBank中已收录伪结核棒状杆菌相似性达97%以上;Balb/c小鼠致病性试验和奶山羊动物回归试验表明分离株有强致病性;药敏试验表明分离菌株对红霉素、环丙沙星、克拉霉素、强力霉素、四环素、头孢唑啉、新霉素、氧氟沙星等多种抗生素敏感,对复方新诺明耐药。
[Abstract]:In order to obtain the pathogenic bacteria in the abscess of sick sheep in a sheep farm in Shaanxi Province, the bacteria were isolated and cultured in the neck pus collected from the sterile sheep, and the morphological characteristics, culture characteristics and biochemical characteristics of the isolated strains were identified. The isolated strains were selected for co-hemolysis test (camp) and PCR amplification of 16s rRNA gene. The phylogenetic tree was constructed by NJ method. The isolated strains were inoculated with Balb / c mice and dairy goats respectively. The drug sensitivity test was used to identify the drug resistance of the isolated strains. The results showed that the morphological characteristics, culture characteristics and biochemical characteristics of the isolated strains were consistent with those of Corynebacterium tuberculosis described in Berger's Manual of bacterial Identification. The results of sequencing showed that the similarity between the isolated strains and pseudotuberculosis Corynebacterium tuberculosis was more than 97% in Balb / c mice and in dairy goat animal regression tests, and the drug sensitivity test showed that the isolates had strong pathogenicity to erythromycin and ciprofloxacin. Clarithromycin, doxycycline, tetracycline, cefazolin, neomycin, ofloxacin and other antibiotics are sensitive and resistant to compound sulfamide.
【作者单位】：西北农林科技大学动物医学院;陕西省富平县动物疫病预防控制中心;
【基金】：陕西省科技统筹创新工程计划项目(K3320215115)
【分类号】：S858.27

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