谷胱甘肽对实验性离体鸡小肠上皮细胞氧化应激的干预研究

发布时间：2018-07-16 23:29

【摘要】：试验旨在研究氧化应激对动物肠道上皮细胞氧化损伤,以及抗氧化剂对上皮细胞的氧化干预。将培养的鸡上皮细胞分为4组,每组设6个重复。对照组A加入等量的三蒸水,试验组B、C、D分别加入黄嘌呤氧化酶(50U/L)和黄嘌呤(12μmol/L),并在C、D组分别加入谷胱甘肽(1,1.5μmol/mL)。结果表明:添加黄嘌呤/黄嘌呤氧化酶(X/X0)导致小肠上皮细胞氧化应激,与对照组比较,显著降低了小肠上皮细胞(IEC)增殖和抗氧化酶的活性,显著提高细胞的脂质氧化和凋亡率(P0.05)。添加不同剂量的谷胱甘肽(GSH)均对IEC的氧化损伤产生保护作用,与B组比较,显著提高了IEC增殖,降低了细胞的脂质氧化和凋亡率;随着GSH添加剂量的增加提高了IEC增殖,但C、D组间比较,抗氧化酶活性差异未达显著水平(P0.05)。
[Abstract]:The purpose of this study was to study oxidative damage of animal intestinal epithelial cells under oxidative stress and oxidative intervention of antioxidants on epithelial cells. The cultured chicken epithelial cells were divided into 4 groups with 6 replicates in each group. In control group A, the same amount of water was added. In group B, xanthine oxidase (50 U / L) and xanthine (12 渭 mol / L) were added, and glutathione (1 1. 5 渭 mol / mL) was added in group C. The results showed that the addition of xanthine / xanthine oxidase (XR / X0) resulted in oxidative stress in intestinal epithelial cells. Compared with the control group, the proliferation and antioxidant enzyme activity of IEC were significantly decreased, and the lipid oxidation and apoptosis rate were significantly increased (P0.05). The effects of glutathione (GSH) at different doses on the oxidative damage of IEC were observed. Compared with group B, GSH significantly increased the proliferation of IEC and decreased the rate of lipid oxidation and apoptosis of IEC cells, and increased the proliferation of IEC with the increase of the amount of GSH additive. However, there was no significant difference in antioxidant enzyme activity between group C and D (P0.05).
【作者单位】：吉林省农业科学院动物营养与饲料研究所;
【基金】：吉林省科技厅自然基金重点项目(20140101024JC)
【分类号】：S831.5

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