复方中药“猪康散”预防用药对人工感染PRRSV猪血清和组织抗氧化功能的影响

发布时间：2018-07-17 08:23

【摘要】：本文研究了不同添加剂量的复方中药“猪康散”预防用药对人工感染PRRSV猪血清和组织抗氧化功能的影响,旨在阐明复方中药“猪康散”对猪繁殖与呼吸综合征的预防效果,为其在养猪业应用推广提供科学依据。试验选取经检测临床健康、体重相近、PRRSV抗原为阴性的35日龄杜长大三元杂交仔猪40头,随机分为4组,每组10头。41-44日龄时中药组(Ⅲ、Ⅳ组)分别在基础日粮中添加0.5%、1.0%“猪康散”,41日龄时除阴性对照组(Ⅰ组)外均滴鼻接种PRRSVSCM株1.0mL。于40、45、50、55、60日龄时,四个组分别随机选取4头仔猪前腔静脉无菌采血,分离血清用于检测抗氧化指标,并在仔猪50、60日龄每组随机选取4头采集肝脏、肺脏、脾脏和肾脏组织,制备组织匀浆,测定组织抗氧化指标。试验结果表明：(1)整个试验期间,与阴性和阳性对照组相比,除40d外,0.5%、1.0%“猪康散”预防用药均能显著提高人工感染PRRSV仔猪血清中SOD活性(P0.01或0.05)；除肝脏和50d肺脏外,1.0%中药预防组各组织中SOD活性均极显著高于两对照组(P0.01)；60d时,0.5%中药预防组肝脏、脾脏和肾脏中SOD活性均极显著高于阳性对照组(P0.01)。(2)与阴性和阳性对照组相比,除40d、0.5%中药预防组第45d、60d外,0.5%、1.0%“猪康散”预防用药均能极显著提高人工感染PRRSV仔猪血清中GSH-Px活性(P0.01)；整个试验期间,除0.5%中药预防组肺和50d脾外,0.5%、1.0%中药预防组仔猪各组织中GSH-Px活力均极显著高于两对照组(P0.01)。(3)与阴性和阳性对照组相比,除40d、0.5%中药预防组第45d外,0.5%、1.0%“猪康散”预防用药均能极显著提高人工感染PRRSV仔猪血清中CAT活性(P0.01)；除阴性对照组60d肺脏外,1.0%中药预防组各组织中CAT活性均显著高于两对照组(P0.01或0.05),除50d肺,0.5%中药预防组各组织中CAT活力均极显著高于阳性对照组(P0.01)。(4)与阴性和阳性对照组相比,除40d和0.5%中药预防组第45d外,0.5%、1.0%“猪康散”预防用药均能极显著提高人工感染PRRSV仔猪血清中T-AOC能力(P0.01)；1.0%中药预防组各组织中T-AOC能力均极显著高于两对照组(P0.01),0.5%中药预防组各组织中T-AOC能力均极显著高于阳性对照组(P0.01)。(5)与阴性和阳性对照组相比,除40d和0.5%中药预防组第45d外,0.5%、1.0%“猪康散”预防用药均能极显著降低人工感染PRRSV仔猪血清MDA含量(P0.01)；除阴性对照组肾、肺脏和60d脾脏外,1.0%中药预防组各组织MDA含量均极显著低于两对照组(P0.01),0.5%中药预防组各组织MDA含量显著低于阳性对照组(P0.01或0.05)。(6)整个试验期间,与阴性和阳性对照组相比,除40d外,0.5%、1.0%“猪康散”预防用药均能极显著提高人工感染PRRSV仔猪血清中抑制·OH能力(P0.01)；除阴性对照组50d肝和50d肾脏外,0.5%、1.0%中药预防组仔猪各组织中抑制OH·能力均显著高于两对照组(P0.01或0.05)。上述结果表明：0.5%和1.0%复方中药“猪康散”预防用药能明显提高人工感染PRRSV仔猪血清和组织SOD、GSH-Px、CAT、T-AOC活力和抑制·OH能力,降低MDA含量的作用,从而提高感染PRRSV仔猪血清和组织抗氧化功能,达到清除自由基、改善机能,提高抗PRRSV的能力,并以1.0%中药添加剂量更佳。
[Abstract]:The effect of the compound Chinese medicine "pig Kang San" on the serum and tissue antioxidant function of PRRSV pigs with artificial infection was studied in this paper. The purpose of this study was to clarify the preventive effect of "pig Kang San" on porcine reproductive and respiratory syndrome, and to provide scientific basis for its application in pig breeding. Healthy, body weight similar, PRRSV antigen negative 35 days old three yuan hybrid piglets were randomly divided into 4 groups, each group of 10.41-44 days of age (Group III, IV) added 0.5%, 1% "pig Kang San" in basal diet, and 41 days old, except for negative control group (group I), inoculated PRRSVSCM strain 1.0mL. at 40,45,50,55,60 days of age In the four groups, the four groups were randomly selected for the aseptic blood sampling of the anterior vena cava of 4 piglets, and the isolated serum was used to detect the antioxidative index. At the age of 50,60 day of the piglets, 4 heads of each group were randomly selected to collect liver, lung, spleen and kidney tissue. The tissue homogenate was prepared and the tissue antioxidant indexes were prepared. The results showed that (1) during the whole experiment, it was negative and positive. Compared with the sex control group, 0.5% and 1% "pigs Kang San" could significantly increase the activity of SOD in the serum of PRRSV piglets with artificial infection (P0.01 or 0.05). Except for the liver and 50D lung, the activity of SOD in each tissue of the prevention group of Chinese medicine was significantly higher than that of the two control group (P0.01), and 0.5% of the liver, spleen and kidneys of the traditional Chinese medicine group were in 60d. The activity of D was significantly higher than that of the positive control group (P0.01). (2) compared with the negative and positive control group, the serum GSH-Px activity (P0.01) of the artificially infected PRRSV piglets was significantly improved, except for 40d, 0.5%, 60d, 0.5%, 1%, and 1% "pig Kang San", except 0.5% Chinese medicine prevention group lung and 50D spleen, 0.5%, 1 during the whole test. The activity of GSH-Px in.0% prevention group was significantly higher than that of the two control group (P0.01). (3) compared with the negative and positive control group, except 40d, 0.5%, 0.5% and 1% "pig Kang San" could significantly improve the CAT activity (P0.01) in the serum of PRRSV piglets, except for the negative control group 60d lung, 1 .0% the activity of CAT in each group of Chinese medicine prevention group was significantly higher than that of the two control group (P0.01 or 0.05). Except for 50D lung, the activity of CAT was significantly higher than that of the positive control group (P0.01). (4) compared with the negative and positive control group, the prevention of the 0.5% and 1% "pig Kang San" was extremely significant except for 40d and 0.5% of the prevention group 45d. Increase the T-AOC ability (P0.01) in the serum of PRRSV piglets with artificial infection. 1% the ability of T-AOC in each group of Chinese medicine prevention group was significantly higher than that of the two control group (P0.01). 0.5% the T-AOC ability in each group of Chinese medicine prevention group was significantly higher than that of the positive control group (P0.01). (5) compared with the negative and positive control group, except 40d and 0.5% traditional Chinese medicine prevention group 45d, 0. 5%, 1% "pig Kang San" can significantly reduce the serum MDA content of PRRSV piglets (P0.01). Except for the kidney, lung and 60d spleen in the negative control group, the content of MDA in each tissue of 1% Chinese medicine prevention group is significantly lower than that of the two control group (P0.01). 0.5% the content of MDA in each group of traditional Chinese medicine prevention group is significantly lower than that of the positive control group (P0.01 or 0.05) (6) during the whole test period, compared with the negative and positive control group, except 40d, 0.5% and 1% "pig Kang San" can significantly improve the inhibitory and OH ability (P0.01) in the serum of artificial infection PRRSV piglets, and the inhibition of OH in each tissue of the piglets of the 50D liver and 50D kidney in the negative control group was significantly higher than that of the two. The control group (P0.01 or 0.05). The results showed that the prophylactic use of "pig Kang San" in compound Chinese medicine of 0.5% and 1% can obviously improve the serum and tissue SOD, GSH-Px, CAT, T-AOC activity and the ability to inhibit the OH, reduce the function of the MDA content, so as to improve the antioxidant function of serum and tissue in the infected PRRSV piglets and achieve the clearance of freedom. It can improve the function and improve the ability of resisting PRRSV, and the dosage of 1% Chinese medicine is better.
【学位授予单位】：四川农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S858.28

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