微量元素添加模式对肉鸡生长性能、微量元素代谢和血浆抗氧化性能的影响

发布时间：2018-07-17 20:10

【摘要】：本试验旨在研究不同微量元素添加模式对肉鸡生长性能、微量元素代谢和血浆抗氧化性能的影响。选取720羽1日龄科宝(Cobb-500)肉鸡,随机分为4个组,每个组10个重复,每个重复18只鸡。行业标准组:按照农业行业标准NY/T 33—2004添加铜、铁、锌和锰。NRC标准组:按照NRC(1994)推荐量添加铜、铁、锌和锰。NRC比例组:实测基础饲粮中铜、铁、锌、锰的含量,以过量最多的铜(相对于NRC标准)的倍数补齐其余3种元素。相对生物学效价组:假设基础饲粮中微量元素的生物学利用率为额外添加硫酸盐的30%,对其含量进行校准后按照NRC比例组的方法添加。微量元素都以硫酸盐形式添加,试验期42 d。结果表明:1)不同微量元素添加模式未对肉鸡生长性能和死亡率造成显著差异(P0.05)。2)21日龄时,NRC比例组肉鸡十二指肠铜转运蛋白1(Ctr1)的mRNA相对表达量显著高于其余各组(P0.05),NRC比例组和相对生物学效价组肉鸡十二指肠二价金属转运蛋白1(DMT1)的mRNA相对表达量显著高于其余2组(P0.05);42日龄时,NRC标准组和相对生物学效价组肉鸡十二指肠DMT1的mRNA相对表达量显著高于其余2组(P0.05);各组间21和42日龄肉鸡十二指肠锌转运蛋白1(Zn T1)和锌转运蛋白5(Zn T5)的mRNA相对表达量没有显著差异(P0.05)。3)21日龄时,NRC比例组和相对生物学效价组肉鸡血浆总抗氧化能力(T-AOC)显著高于其余2组(P0.05);42日龄时,与相对生物学效价组相比,NRC比例组肉鸡血浆过氧化氢酶(CAT)活性显著下降(P0.05)。4)粪便中微量元素浓度和饲粮的微量元素添加浓度存在显著的正相关关系(P0.05)。由此可见,从微量元素吸收效率和肉鸡血浆抗氧化性能来看,考虑基础饲粮微量元素相对生物学效价并按NRC比例添加是更适宜的添加模式,同时减少了粪便中微量元素的排泄。
[Abstract]:The purpose of this study was to study the effects of different microelement supplementation modes on growth performance, trace element metabolism and plasma antioxidant activity in broilers. Cobb-500 broilers were randomly divided into 4 groups with 10 replicates in each group and 18 chickens in each group. Industry standard group: according to the agricultural industry standard NYP / T33-2004 adding copper, iron, zinc and manganese. NRC standard group: according to the recommended amount of NRC (1994) added copper, iron, zinc and manganese 路NRC ratio group: measured the content of copper, iron, zinc, manganese in basic diet, Fill the remaining three elements with a multiple of the maximum amount of copper (relative to the NRC standard). Relative biological titer group: assuming that the biological utilization rate of trace elements in the basal diet was 30% of the additional sulfate, the contents were calibrated and added according to the method of NRC proportion group. Trace elements were added in the form of sulfate for 42 days. The results showed that there was no significant difference in growth performance and mortality of broilers with different microelement supplementation patterns (P0.05). The relative expression of Ctr1 mRNA in duodenum of broiler chickens at 21 days of age was significantly higher than that in other groups (P0.05). The relative expression of DMT1 mRNA in the duodenum of broilers with and without biological titer was significantly higher than that of the other two groups (P0.05) at 42 days of age, and the relative expression of DMT1 mRNA in duodenum of broiler chickens with NRC standard group and relative biological titer group was significantly higher than that of the other two groups (P0.05). There was no significant difference in the relative expression of zinc transporter 1 (Zn T1) and zinc transporter 5 (Zn T 5) mRNA between the 21 and 42 day old broilers (P0.05). The plasma total antioxidant capacity (T-AOC) of broilers was significantly higher than that of the other two groups (P0.05) at 42 days of age. Compared with the relative biological titer group, the plasma catalase (cat) activity in NRC ratio group decreased significantly (P0.05). There was a significant positive correlation between trace element concentration in feces and dietary trace element concentration (P0.05). Therefore, from the point of view of the absorption efficiency of trace elements and the antioxidant activity of plasma, it is more appropriate to consider the relative biological titer of trace elements in basal diet and add them in proportion to NRC, and to reduce the excretion of trace elements in feces.
【作者单位】：西北农林科技大学动物科技学院;
【基金】：陕西省科技统筹创新工程计划项目(2015KTCQ02-19,2015KTCL02-10)
【分类号】：S831.5

【相似文献】