中国荷斯坦奶牛血液乳成分前体物及相关激素的变化规律及其与乳成分的关联分析
发布时间：2018-07-18 14:34
【摘要】：本研究选用荷斯坦奶牛为试验动物,通过检测分析胎次和泌乳阶段对奶牛血液中乳成分前体物和相关激素的影响及奶牛产奶性能及乳成分与血液中乳成分前体物及相关激素的关联分析,旨在为乳成分合成调控机制的研究,奶牛营养状况的科学评价和奶牛日粮的合理配制提供理论依据。内容一:旨在研究胎次和泌乳阶段对荷斯坦奶牛血液中乳成分前体物及泌乳相关激素的影响。(方法)选取不同泌乳阶段的1-4胎奶牛60头饲喂相同TMR日粮,每隔15天采集血液。血液检测参数有血清葡萄糖(GLU)、甘油三酯(TG)、非酯化脂肪酸(NEFA)、β-羟丁酸(BHBA)、总氨基酸(T-AA)5种乳成分合成前体物MCP及甲状腺素(T4)、生长激素(GH)、皮质醇(COR)、胰岛素(INS)、催乳素(PRL)、孕酮(PRG)6种激素。利用PROC UNIVERIATE进行正态分布检验,利用PROC MIXED线性模型评估胎次、泌乳阶段的固定效应。(结果)结果表明,胎次可显著影响泌乳奶牛血液中INS及PRL含量(P0.01),而对COR、GH、PRG、T4及5个乳成分前体物MCP含量无显著影响(P0.05);血液中GLU浓度随着泌乳阶段逐渐递增,且泌乳早期GLU浓度显著低于其他泌乳阶段(P0.05),而泌乳早期BHBA和NEFA的浓度显著高于泌乳高峰期及中后期(P0.05),同时T-AA浓度在泌乳中期(101-200d)显著升高(P0.05);泌乳阶段对奶牛血液中COR、GH、PRG、T4和INS含量均有显著的影响(P0.01或P0.05),但对PRL无显著影响(P0.05),其中泌乳前期COR的浓度显著高于泌乳中后期(P0.01),而GH、PRG和T4均在泌乳后期显著升高(P0.01)。(结论)不同胎次和泌乳阶段的荷斯坦奶牛血液乳成分前体物及相关激素含量存在差异。研究成果将提高利用生化检测指标评估泌乳荷斯坦奶牛代谢状态的准确性,可以作为奶牛养殖者监测奶牛健康状态的工具。内容二:旨在研究奶牛产奶性能及乳成分与血液中乳成分前体物及相关激素的变化规律和关联性。(方法)选用试验一的2-4胎荷斯坦奶牛37头。采集奶样后及时送到DHI检测中心检测常规乳成分。采用SAS9.2中PROC CORR过程,对乳成分及血液中乳成分前体物及相关激素指标间进行典型相关性分析。(结果)乳蛋白率和血液中T-AA呈现显著的正相关(P=0.0081),乳脂率和NEFA、BHBA之间的呈现显著的正相关(P0.0001)乳糖率和GLU之间的无显著性关系(P=0.1150);GH与乳产量、乳脂率之间呈极显著负相关(P0.0001),GH与乳蛋白率呈极显著正相关(P0.0001),相关系数R=0.6194。GH与乳糖率无显著相关性(P=0.7317)。GH与乳脂前体物NEFA、BHBA之间呈极显著负相关(P0.0001),GH与乳糖前体物GLU之间呈显著正相关(P0.0001)。COR与乳产量、乳脂率之间呈极显著正相关(P0.0001),COR与乳蛋白率呈极显著负相关(P0.0001),COR与乳脂前体物NEFA、BHBA之间呈极显著正相关(P0.0001),COR与乳糖前体物GLU之间呈显著负相关(P0.0001),T4与乳脂肪率之间呈极显著正相关(P=0.0070);与BHBA、NEFA之间极显著负相关相关(P0.0001),(结论)通过对乳成分、血液中的乳成分前体物及相关激素相关性分析发现,乳成分前体物对乳的合成影响存在互作关系,即任何一种乳成分前体物变化不但会对对应的乳成分产生影响,而且也会影响其它乳成分的合成,一种乳成分的合成需要多种激素的共同调控。
[Abstract]:In this study, Holstein cows were selected as experimental animals, and the relationship between milk composition precursors and related hormones in the blood of dairy cows and the relationship between milk composition and milk composition precursor and related hormones in the blood of dairy cows were analyzed and analyzed. The aim of this study was to study the regulation mechanism of milk composition synthesis, and the nutrition of dairy cows. The scientific evaluation of the situation and the rational preparation of dairy cows provided a theoretical basis. Content 1: the purpose of the study was to study the effects of the birth and lactation stages on the milk composition precursors and lactation related hormones in the blood of Holstein cows. (Methods) 60 cows fed the same TMR diet at different lactation stages were fed with the same TMR diet every 15 days. The parameters include serum glucose (GLU), triglyceride (TG), non esterified fatty acid (NEFA), beta hydroxybutyric acid (BHBA), and total amino acid (T-AA) 5 kinds of milk ingredients, MCP and thyroxine (T4), growth hormone (GH), cortisol (COR), insulin (INS), prolactin (PRL), and progesterone (PRG) 6 hormones. C MIXED linear model was used to evaluate the fixed effect of the birth and lactation stage. (results) the results showed that the number of INS and PRL in the blood of lactating dairy cows (P0.01) was significantly affected by the parity, while COR, GH, PRG, T4 and the MCP content of 5 dairy precursors were not significantly affected (P0.05), and the concentration of GLU in the blood gradually increased with the lactation stage, and the concentration in the early lactation stage was significant. The concentration of BHBA and NEFA in early lactation was significantly higher than that in the peak period and the middle and late period of lactation (P0.05), while the concentration of T-AA increased significantly (P0.05) in the middle period of lactation (P0.05), and the lactation stage had significant influence on COR, GH, PRG, T4 and INS content in the blood of dairy cows. 5), the concentration of COR in prolactation was significantly higher than that in late lactation (P0.01), while GH, PRG and T4 were significantly increased in late lactation (P0.01). (conclusion) there was a difference in the content of blood milk components and related hormones in Holstein cows at different parity and lactation stages. The results will improve the evaluation of lactating Holstein using biochemical markers. The accuracy of metabolic state of dairy cows can be used as a tool for dairy cattle to monitor the health of dairy cows. Content two: the aim of this study is to study the changes in milk performance and milk ingredients and the related hormones in the milk components and related hormones in the blood. (Methods) select 37 cows of 2-4 Holstein cows in test one. After collecting milk samples, they are sent to DH in time. The I test center was used to detect the routine milk composition. Using the PROC CORR process in SAS9.2, the milk composition and the milk composition precursor and the related hormone indexes were analyzed. (results) the milk protein rate and the blood T-AA showed significant positive correlation (P=0.0081), the milk fat rate and the NEFA, BHBA showed a significant positive correlation (P0.0001) milk. There was a significant negative correlation between sugar rate and GLU (P=0.1150); GH had a very significant negative correlation with milk yield and milk fat rate (P0.0001), GH had a very significant positive correlation with milk protein rate (P0.0001), and the correlation coefficient R=0.6194.GH had no significant correlation with lactose rate (P=0.7317).GH and NEFA, BHBA between milk fat precursors (P0.0001). There was a significant positive correlation between GLU and milk yield, milk yield and milk fat rate (P0.0001), COR was significantly negatively correlated with milk protein rate (P0.0001), COR and milk fat precursor NEFA, BHBA showed significant positive correlation (P0.0001), COR was negatively correlated with lactose precursor GLU (P0.0001), and milk fat was significantly correlated with milk fat. There is a very significant positive correlation between the rates (P=0.0070) and a significant negative correlation with BHBA and NEFA (P0.0001). (conclusion) through the correlation analysis of milk ingredients, milk ingredients precursors and related hormones in the blood, it is found that there is an interaction relationship between milk composition precursors and milk composition. The composition of milk should be affected, and the composition of other milk components will also be affected. The synthesis of a milk component requires the co regulation of multiple hormones.
【学位授予单位】：中国农业科学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S823
本文编号：2132205
[Abstract]:In this study, Holstein cows were selected as experimental animals, and the relationship between milk composition precursors and related hormones in the blood of dairy cows and the relationship between milk composition and milk composition precursor and related hormones in the blood of dairy cows were analyzed and analyzed. The aim of this study was to study the regulation mechanism of milk composition synthesis, and the nutrition of dairy cows. The scientific evaluation of the situation and the rational preparation of dairy cows provided a theoretical basis. Content 1: the purpose of the study was to study the effects of the birth and lactation stages on the milk composition precursors and lactation related hormones in the blood of Holstein cows. (Methods) 60 cows fed the same TMR diet at different lactation stages were fed with the same TMR diet every 15 days. The parameters include serum glucose (GLU), triglyceride (TG), non esterified fatty acid (NEFA), beta hydroxybutyric acid (BHBA), and total amino acid (T-AA) 5 kinds of milk ingredients, MCP and thyroxine (T4), growth hormone (GH), cortisol (COR), insulin (INS), prolactin (PRL), and progesterone (PRG) 6 hormones. C MIXED linear model was used to evaluate the fixed effect of the birth and lactation stage. (results) the results showed that the number of INS and PRL in the blood of lactating dairy cows (P0.01) was significantly affected by the parity, while COR, GH, PRG, T4 and the MCP content of 5 dairy precursors were not significantly affected (P0.05), and the concentration of GLU in the blood gradually increased with the lactation stage, and the concentration in the early lactation stage was significant. The concentration of BHBA and NEFA in early lactation was significantly higher than that in the peak period and the middle and late period of lactation (P0.05), while the concentration of T-AA increased significantly (P0.05) in the middle period of lactation (P0.05), and the lactation stage had significant influence on COR, GH, PRG, T4 and INS content in the blood of dairy cows. 5), the concentration of COR in prolactation was significantly higher than that in late lactation (P0.01), while GH, PRG and T4 were significantly increased in late lactation (P0.01). (conclusion) there was a difference in the content of blood milk components and related hormones in Holstein cows at different parity and lactation stages. The results will improve the evaluation of lactating Holstein using biochemical markers. The accuracy of metabolic state of dairy cows can be used as a tool for dairy cattle to monitor the health of dairy cows. Content two: the aim of this study is to study the changes in milk performance and milk ingredients and the related hormones in the milk components and related hormones in the blood. (Methods) select 37 cows of 2-4 Holstein cows in test one. After collecting milk samples, they are sent to DH in time. The I test center was used to detect the routine milk composition. Using the PROC CORR process in SAS9.2, the milk composition and the milk composition precursor and the related hormone indexes were analyzed. (results) the milk protein rate and the blood T-AA showed significant positive correlation (P=0.0081), the milk fat rate and the NEFA, BHBA showed a significant positive correlation (P0.0001) milk. There was a significant negative correlation between sugar rate and GLU (P=0.1150); GH had a very significant negative correlation with milk yield and milk fat rate (P0.0001), GH had a very significant positive correlation with milk protein rate (P0.0001), and the correlation coefficient R=0.6194.GH had no significant correlation with lactose rate (P=0.7317).GH and NEFA, BHBA between milk fat precursors (P0.0001). There was a significant positive correlation between GLU and milk yield, milk yield and milk fat rate (P0.0001), COR was significantly negatively correlated with milk protein rate (P0.0001), COR and milk fat precursor NEFA, BHBA showed significant positive correlation (P0.0001), COR was negatively correlated with lactose precursor GLU (P0.0001), and milk fat was significantly correlated with milk fat. There is a very significant positive correlation between the rates (P=0.0070) and a significant negative correlation with BHBA and NEFA (P0.0001). (conclusion) through the correlation analysis of milk ingredients, milk ingredients precursors and related hormones in the blood, it is found that there is an interaction relationship between milk composition precursors and milk composition. The composition of milk should be affected, and the composition of other milk components will also be affected. The synthesis of a milk component requires the co regulation of multiple hormones.
【学位授予单位】：中国农业科学院
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S823
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