我国2002-2016年间鸡传染性支气管炎病毒基因组序列重组分析

发布时间：2018-07-20 08:59

【摘要】：【目的】传染性支气管炎病毒(Infectious bronchitis virus,IBV)主要引起鸡群呼吸道与肾脏疾病,是影响养禽业重要的病毒性病原之一。了解我国IBV的流行及基因重组情况。【方法】收集Gen Bank中我国2002-2016年间分离的92株IBV S1基因及55株IBV基因组序列,并对这些序列进行比对分析。【结果】IBV S1基因序列分析结果表明,2002-2016年间我国流行的92株IBV可以分为13个基因型,包括QX、4/91、Mass、tl/CH/LDT3/03、CK/CH/LSC/99I、TW-I、TW-II、TC07-2、Ck/CH/LDL/97I、N1/62-associated、Arkansas、New-I及一个新鉴定的基因分支New-II。值得注意的是,属于美国相关基因分支的ck/CH/LSD/110712已在我国出现。RDP4方法进行重组分析显示,新出现的基因分支New-II病毒S1基因来源于tl/CH/LDT3/03型与QX型毒株重组,我国2002-2016年间流行的55株IBV中有52株IBV基因组存在重组事件,其中25个IBV分离株基因组中发现有疫苗型(Mass,tl/CH/LDT3/03及4/91型等)病毒基因组片段的重组,这一结果在Sim Plot分析中进一步得到确认。【结论】根据生物信息学分析结果,证明流行于我国的IBV基因型众多,疫苗毒株基因频繁参与了IBV基因重组,导致IBV新的基因型或变异株出现,提示在防控IB时要注意合理使用IBV疫苗。
[Abstract]:[objective] Infectious bronchitis virus (IBV) mainly causes respiratory and renal diseases in chickens and is one of the most important viral pathogens affecting poultry industry. To understand the prevalence and gene recombination of IBV in China. [methods] 92 IBV S1 genes and 55 IBV genomic sequences isolated from GenBank from 2002 to 2016 were collected. The results showed that the 92 IBV strains prevalent in China during 2002-2016 could be divided into 13 genotypes, including QXT 4 / 91 Masstl-r / CHLDT 3 / 03 / CHLSC99I / TW-IK / TW-IITC07-2CkP / CHLDL97IN 1 / 62-associated INA New-I and a newly identified gene branch New-II. It is worth noting that ck/ Ch / LSD / 110712, which belongs to the related gene branch of the United States, has been recombined with the. RDP4 method in China. The new gene branch, New-II virus S1 gene, originated from the recombination of tl/ Ch / LDT3 / 03 and QX strains. Among the 55 IBV strains prevalent in China from 2002 to 2016, 52 IBV genomes had recombinant events, of which 25 IBV isolates were found to have recombinant genome fragments of vaccine type (Masstlr / Ch / LDT _ 3 / 03 and 4 / 91, etc.). This result was further confirmed by Sim blot analysis. [conclusion] according to the results of bioinformatics analysis, there are many IBV genotypes prevalent in China, and the genes of vaccine strains frequently participate in the recombination of IBV genes. The emergence of new genotypes or variant strains of IBV suggests that the rational use of IBV vaccine should be paid attention to in the prevention and control of IB.
【作者单位】：禽类预防医学省部共建教育部重点实验室扬州大学兽医学院;
【基金】：国家基础研究计划项目(No.2013FY113300-4) 国家重点研发计划项目(No.2016YFD0500803) 江苏省优势学科项目江苏省动物重要疫病与人兽共患病防控协同创新中心项目~~
【分类号】：S852.65

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