不同水平紫苏籽对土种蛋鸡生产性能、脂质指标和免疫功能的影响
发布时间：2018-07-21 12:42
【摘要】：本试验旨在研究不同水平紫苏添加剂对土蛋鸡生产性能、脂质指标、免疫功能的影响,为将其应用于养禽生产提供理论参考。饲养试验选取28周龄体重相近的健康产蛋高峰期土蛋鸡360只,随机分成4组,每组3个重复,每个重复30只,试验鸡自由饮水。1组为对照组,饲喂基础日粮,2、3、4组在基础日粮中分别添加1%、2%、3%的加工处理后的紫苏籽,饲养试验为期8周(预饲一周)。全程平均每只鸡固定采食量83.33g/d,以每组每个重复为单位,计算生产性能指标：日均产蛋量、产蛋率、平均蛋重、料蛋比、种蛋合格率,蛋比重、蛋壳强度、蛋壳厚度、蛋形指数、浓蛋白高度、哈氏单位；测定与脂类代谢相关指标：血清总胆固醇、甘油三酯、高密度脂蛋白、低密度脂蛋白,蛋黄胆固醇、蛋黄甘油三酯；测定与免疫机能相关指标：总蛋白、白蛋白、白球比,H5型禽流感抗体滴度、H9型禽流感抗体滴度、新城疫抗体滴度。实验结果如下：1紫苏籽对生产性能的影响：添加紫苏籽能提高种蛋鸡生产性能,3组日均产蛋量显著高于(P0.05)其它组,在产蛋率、平均蛋重、种蛋合格率方面,实验组均显著高于(P0.05)对照组,料蛋比实验组显著低于(P0.05)对照组；蛋品质方面,除3组蛋重和蛋壳强度显著高于(P0.05)其它组,其它指标均无显著差异,但随着紫苏籽添加水平的增高,所有指标均有上升趋势。2紫苏籽对脂质指标的影响：血脂方面：2组与对照组脂质指标均无显著差异,3组与4组和对照组各项指标相比,均存在显著差异(P0.05),3、4组在血清总胆固醇、甘油三酯、高密度脂蛋白方面均显著高于(P0.05)对照组,且随着紫苏籽添加量的增高,有上升趋势,低密度脂蛋白显著低于对照组,且随着紫苏籽添加量的增高,有下降趋势；蛋脂方面：试验组蛋黄胆固醇均显著低于(P0.05)对照组,2组蛋黄甘油三酯与对照组无显著差异,3、4组蛋黄甘油三酯均显著低于(P0.05)对照组,且随着紫苏籽添加水平的增加,与对照组相比各指标均有下降趋势；酶活方面：1、2组FAS酶活无显著差异,3、4组FAS酶活显著低于(P0.05)对照组,试验组HMG-CoA酶活均显著低于(P0.05)对照组。3紫苏籽对免疫功能的影响：免疫蛋白方面：试验组总蛋白均显著高于(P0.05)对照组,各组问白蛋白无显著差异,4组白球比显著高于(P0.05)其它组,1、2、3组白球比无显著差异,但试验组较对照组有上升趋势；抗体滴度方面：3、4组血清H5、H9型禽流感抗体滴度均显著高于(P0.05)对照组,2组H5型禽流感抗体滴度显著高于(P0.05)对照组,其余各组差异不显著。以上结论表明：2%的紫苏籽能提高土种蛋鸡生产性能,降低血脂、蛋脂,提高机体免疫能力。
[Abstract]:The purpose of this study was to study the effects of different levels of perilla additive on the performance, lipid index and immune function of laying hens in order to provide a theoretical reference for their application in poultry production. In the feeding experiment, 360 indigenous laying hens with similar body weight at 28 weeks were randomly divided into 4 groups, 3 replicates in each group and 30 in each. The control group was treated with free drinking water. The 4 groups were fed with 3% of processed perilla seeds in the basal diet. The feeding experiment lasted 8 weeks (one week before feeding). The average fixed feed intake per chicken was 83.33 g / d, and the production performance indexes were calculated as follows: average daily egg production, egg laying rate, average egg weight, feed to egg ratio, qualified rate, egg specific gravity, eggshell strength, eggshell thickness, egg shape index. Determination of serum total cholesterol, triglyceride, high density lipoprotein, low density lipoprotein, egg yolk cholesterol, triglyceride of egg yolk; The related indexes of immune function were determined: total protein, titer of H5 avian influenza antibody, titer of H9 avian influenza antibody and Newcastle disease antibody titer. The results were as follows: the effect of perilla seed on production performance was as follows: adding perilla seed could improve the production performance of laying hens. The average daily egg production of the three groups was significantly higher than that of the other groups (P0.05), and the egg laying rate, average egg weight and egg qualified rate were significantly higher than those of the other groups. The egg quality of the experimental group was significantly higher than that of the control group (P0.05), the egg weight and eggshell strength of the three groups were significantly higher than that of the other groups (P0.05), but there was no significant difference in other indexes. However, with the increase of perilla seed supplementation level, all indexes showed an upward trend. 2 the effect of perilla seed on lipid index: there was no significant difference in lipid index between group 2 and control group in blood lipids, the indexes of lipid in group 3, group 4 and control group were higher than those in group 3 and group 4, respectively. There were significant differences (P0.05) in serum total cholesterol, triglyceride, high-density lipoprotein in the four groups were significantly higher than those in the control group (P0.05), and with the increase of perilla seed addition, there was an upward trend, low density lipoprotein was significantly lower than that of the control group. Egg fat: the cholesterol of egg yolk in the trial group was significantly lower than that in the control group (P0.05). There was no significant difference between the control group and the control group in the triglyceride content of egg yolk triglyceride in the trial group and in the control group, which were significantly lower than those in the control group (P0.05), and there was no significant difference between the control group and the control group in the content of triglyceride of egg yolk. With the increase of perilla seed supplementation level, each index showed a downward trend compared with the control group, and there was no significant difference in the activity of FAS enzyme between the two groups. The FAS enzyme activity of the 4 groups was significantly lower than that of the control group (P0.05), and there was no significant difference in the activity of FAS between the two groups (P0.05). The activity of HMG-CoA enzyme in the experimental group was significantly lower than that in the control group (P0.05). The effect of perilla seed on immune function in the control group: the total protein in the test group was significantly higher than that in the control group (P0.05). There was no significant difference in the white ball ratio between the four groups (P0.05), but there was no significant difference in the white ball ratio of the other groups (P0.05), but there was an upward trend in the test group compared with the control group. The titer of serum H5 H9 type avian influenza antibody in the control group was significantly higher than that in the control group (P0.05), but there was no significant difference among the other groups. The results showed that 2% perilla seed could improve the performance of native layer hens, reduce blood lipid and egg fat, and improve immune ability.
【学位授予单位】：广西大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S831.5
[Abstract]:The purpose of this study was to study the effects of different levels of perilla additive on the performance, lipid index and immune function of laying hens in order to provide a theoretical reference for their application in poultry production. In the feeding experiment, 360 indigenous laying hens with similar body weight at 28 weeks were randomly divided into 4 groups, 3 replicates in each group and 30 in each. The control group was treated with free drinking water. The 4 groups were fed with 3% of processed perilla seeds in the basal diet. The feeding experiment lasted 8 weeks (one week before feeding). The average fixed feed intake per chicken was 83.33 g / d, and the production performance indexes were calculated as follows: average daily egg production, egg laying rate, average egg weight, feed to egg ratio, qualified rate, egg specific gravity, eggshell strength, eggshell thickness, egg shape index. Determination of serum total cholesterol, triglyceride, high density lipoprotein, low density lipoprotein, egg yolk cholesterol, triglyceride of egg yolk; The related indexes of immune function were determined: total protein, titer of H5 avian influenza antibody, titer of H9 avian influenza antibody and Newcastle disease antibody titer. The results were as follows: the effect of perilla seed on production performance was as follows: adding perilla seed could improve the production performance of laying hens. The average daily egg production of the three groups was significantly higher than that of the other groups (P0.05), and the egg laying rate, average egg weight and egg qualified rate were significantly higher than those of the other groups. The egg quality of the experimental group was significantly higher than that of the control group (P0.05), the egg weight and eggshell strength of the three groups were significantly higher than that of the other groups (P0.05), but there was no significant difference in other indexes. However, with the increase of perilla seed supplementation level, all indexes showed an upward trend. 2 the effect of perilla seed on lipid index: there was no significant difference in lipid index between group 2 and control group in blood lipids, the indexes of lipid in group 3, group 4 and control group were higher than those in group 3 and group 4, respectively. There were significant differences (P0.05) in serum total cholesterol, triglyceride, high-density lipoprotein in the four groups were significantly higher than those in the control group (P0.05), and with the increase of perilla seed addition, there was an upward trend, low density lipoprotein was significantly lower than that of the control group. Egg fat: the cholesterol of egg yolk in the trial group was significantly lower than that in the control group (P0.05). There was no significant difference between the control group and the control group in the triglyceride content of egg yolk triglyceride in the trial group and in the control group, which were significantly lower than those in the control group (P0.05), and there was no significant difference between the control group and the control group in the content of triglyceride of egg yolk. With the increase of perilla seed supplementation level, each index showed a downward trend compared with the control group, and there was no significant difference in the activity of FAS enzyme between the two groups. The FAS enzyme activity of the 4 groups was significantly lower than that of the control group (P0.05), and there was no significant difference in the activity of FAS between the two groups (P0.05). The activity of HMG-CoA enzyme in the experimental group was significantly lower than that in the control group (P0.05). The effect of perilla seed on immune function in the control group: the total protein in the test group was significantly higher than that in the control group (P0.05). There was no significant difference in the white ball ratio between the four groups (P0.05), but there was no significant difference in the white ball ratio of the other groups (P0.05), but there was an upward trend in the test group compared with the control group. The titer of serum H5 H9 type avian influenza antibody in the control group was significantly higher than that in the control group (P0.05), but there was no significant difference among the other groups. The results showed that 2% perilla seed could improve the performance of native layer hens, reduce blood lipid and egg fat, and improve immune ability.
【学位授予单位】：广西大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S831.5
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