梅花鹿茸顶端组织中骨形成蛋白和转移生长因子的分布

发布时间：2018-07-21 14:10

【摘要】：目的:研究鹿茸顶部组织中骨形态发生蛋白(BMPs)、转化生长因子-β(TGF-β)的分布,探索鹿茸生长发育机制。方法:采用免疫组织化学技术,观察和半定量分析了BMPs、TGF-β1在梅花鹿茸顶部组织细胞中的分布情况。结果:BMPs在表皮的基底细胞、毛囊细胞中均有强烈表达,真皮内的细胞、间充质细胞和前成软骨细胞无阳性反应,随着细胞的不断分化阳性细胞增加,软骨细胞有强阳性反应。从表皮向内,经真皮到间充质层,细胞外间质的BMPs棕黄色阳性反应渐渐减弱,平均总累计光密度值(IOD)由最高降至最低,从前成软骨层、过渡层到软骨层IOD又逐渐升高,但仍明显低于表皮层(P0.01)。TGF-β1在各层均有分布,IOD值存在显著差异(P0.05),从大到小按软骨层、过渡层、茸皮血管层、前成软骨层、间充质层、表皮层、毛囊层排列,血窦、血管和管膜形成区表达较强。结论:在梅花鹿茸顶部BMPs主要分布于表皮、毛囊和软骨层中,但表皮中的表达量最高,TGF-β1以不同的表达量分布于各层。
[Abstract]:Aim: to study the distribution of bone morphogenetic proteins (BMPs) and transforming growth factor- 尾 (TGF- 尾) in pilose antler top tissue and to explore the mechanism of growth and development of pilose antler. Methods: immunohistochemical technique was used to observe and semi-quantitatively analyze the distribution of BMPsTGF- 尾 1 in the tissues of the top of sika velvet antler. Results there was strong expression of VBMPs in basal cells and hair follicle cells of epidermis. There was no positive reaction in dermal cells, mesenchymal cells and prechondroblasts. With the increase of positive cells in differentiation, chondrocytes showed strong positive reaction. From the epidermis to the mesenchymal layer, the brown positive reaction of BMPs in the extracellular stroma gradually decreased, the average total cumulative optical density (IOD) decreased from the highest to the lowest, and the IOD increased gradually from the former cartilage layer to the transitional layer to the cartilage layer. But it was still significantly lower than that of the epidermis (P0.01). TGF- 尾 1 was significantly lower than that of the epidermis (P0.01). There were significant differences in the distribution of IOD in all layers (P0.05), from large to small according to the cartilage layer, transitional layer, antler skin vascular layer, anterior chondrogenic layer, mesenchymal layer, epidermis, hair follicle layer, and blood sinus. The expression of vascular and membranous formation area was strong. Conclusion: BMPs are mainly distributed in epidermis, hair follicles and chondrocytes at the top of velvet antler, but the highest expression of TGF- 尾 1 is found in epidermis.
【作者单位】：山西省医药与生命科学研究院;
【基金】：山西省科技攻关项目(20080312013)
【分类号】：S825

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