龙岩市猪流行性乙型脑炎的流行病学调查

发布时间：2018-07-21 15:15

【摘要】：为了解福建省龙岩市猪乙型脑炎病毒(JEV)的隐性感染情况和使用疫苗后的血清抗体水平,采用间接酶联免疫吸附试验(ELISA)对龙岩市2011—2014年规模化猪场和农村散养户共858份猪血清样本进行了JEV的抗体水平检测,并对临床疑似猪乙型脑炎的41个病例进行了聚合酶链式反应(PCR)检测。结果表明:已免疫规模猪场和农村散养户JEV的血清抗体阳性率分别为72.17%和57.72%,规模场JEV的免疫合格率要好于散养户(P0.01);免疫猪群JEV的血清抗体阳性率(69.71%)与未免疫猪群JEV的血清抗体阳性率(68.89%)相比差异不显著(P0.05);2011—2014年检测规模猪场和农村散养户JEV血清抗体水平总体上差异不显著(P0.05);PCR检测41个疑似猪乙型脑炎病例,确诊阳性21份,阳性率为51.22%,其中农村散养户猪只的JEV核酸阳性率显著高于规模场(P0.01)。说明龙岩市JEV的免疫效果不理想,JEV感染仍较严重,应引起重视。
[Abstract]:In order to understand the recessive infection of swine encephalitis virus (JEV) in Longyan City, Fujian Province and the level of serum antibody after using the vaccine, Indirect enzyme-linked immunosorbent assay (Elisa) was used to detect the antibody level of 858 pig serum samples from large-scale pig farms and rural free-range farmers in Longyan City from 2011 to 2014. Polymerase chain reaction (PCR) was performed in 41 cases of suspected swine encephalitis B. The results showed that the positive rates of serum antibodies in the immunized pig farms and rural scattered households were 72.17% and 57.72%, respectively. The qualified rate of JEV in the scale field was better than that in the bulk households (P0.01), and the positive rate of serum antibodies in the immunized pigs (69.71%) was higher than that in the unimmunized pigs (P0.01). The positive rate of serum antibody of JEV (68.89%) had no significant difference (P0.05). There was no significant difference in serum antibody level of JEV between 2011 and 2014 (P0.05). PCR was used to detect 41 suspected cases of Japanese encephalitis. The positive rate of JEV nucleic acid was 51.22%, and the positive rate of JEV nucleic acid was significantly higher than that of scale field (P0.01). The results showed that the immune effect of JEV in Longyan City was still serious, which should be paid more attention to.
【作者单位】：龙岩学院生命科学学院;家畜传染病防治与生物技术福建省重点实验室;福建省生猪疫病防控工程技术研究中心;
【分类号】：S851.31+3;;S852.65+1

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