兔斯氏艾美耳球虫感染模型的建立及病理学初步研究

发布时间：2018-07-23 08:26

【摘要】：建立兔斯氏艾美耳球虫感染模型,为研究斯氏艾美耳球虫感染兔的有效防治方法提供适合的动物模型。2月龄普通级新西兰兔16只,分组单笼饲养。从自然感染的肝脏中收集斯氏艾美耳球虫卵囊,培养孢子化,孢子化的卵囊1.0×105个/mL,3mL孢子化卵囊口服感染新西兰兔,建立兔斯氏艾美耳球虫感染模型,并对感染1个月后的新西兰兔的肝脏、十二指肠进行病理学观察比较。结果显示:斯氏艾美耳球虫对新西兰兔部分致病性观察发现,新西兰兔在感染后出现明显的临床症状,严重制约了其生长发育。形态学观察发现肝脏感染严重,表面布满白色结节,胆囊中胆汁浓稠,颜色呈金黄色,而十二指肠虽未感染球虫,却也有较显著的病变。病理学观察发现肝脏中存在大量斯氏艾美耳球虫,十二指肠中不存在球虫,但组织结构亦被破坏,十二指肠绒毛遭到严重破坏。结果表明:本试验成功建立了兔斯氏艾美耳球虫感染模型。
[Abstract]:In order to provide a suitable animal model for the prevention and treatment of Eimeria skrjabini infection in rabbits, 16 New Zealand rabbits of the average age of .2 months were raised in a single cage. The oocysts of Eimeria skrjabini were collected from the naturally infected liver, and the sporulation of oocysts was cultured. The sporulated oocysts of 1.0 脳 105 / mL / mL oocysts were orally infused into New Zealand rabbits, and the infection model of Eimeria sibiricus was established. The liver and duodenum of New Zealand rabbits were observed and compared one month after infection. The results showed that there were obvious clinical symptoms in New Zealand rabbits after infection, which seriously restricted the growth and development of New Zealand rabbits. Morphological observation showed that the liver infection was serious, the surface was covered with white nodules, the bile in the gallbladder was thick and the color was golden, but the duodenum was not infected with coccidiosis, but also had obvious pathological changes. Pathological observation showed that there were a large number of Eimeria sterni in the liver and no coccidia in the duodenum, but the tissue structure was also destroyed, and the villi of the duodenum were seriously damaged. The results showed that the infection model of Eimeria sibiricus was successfully established in this experiment.
【作者单位】：南通大学实验动物中心;
【基金】：国家自然科学基金资助项目(31301926) 江苏省自然科学基金资助项目(BK20130388)
【分类号】：S858.291

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