理化因素对我国新城疫病毒流行株感染力的影响
发布时间：2018-07-23 10:43
【摘要】：目的了解水环境的理化因素对国内新城疫病毒流行株感染力的影响,以及新城疫病毒强、弱毒株对理化因素耐受性差别。方法测定3株NDV强毒株和3株弱毒株在不同温度(20、37和54℃)、盐度(0、15和30g/L)和pH(4.4、5.4和7.4)条件下保存时,病毒的鸡胚半数感染量(EID50)随时间的动态变化。使用线性回归方程分析不同理化条件下病毒的感染持续期。结果对照组用磷酸盐缓冲液(pH 7.4和20℃)保存时,病毒EID50保持相对稳定,RT(病毒感染力降低90%所需天数)可达26.25~64.52d;在30g/L NaCl条件下,病毒RT为5.78~24.51d;在pH 4.4条件下病毒的EID50下降较快,RT在4.54~10.66d之间;在57℃条件下,病毒的EID50迅速下降,RT1d。NDV强、弱毒株RT值相似。结论酸度增加和盐度增高可一定程度上缩短病毒在水中的存活时间,而水温增高则导致病毒感染力的迅速降低。病毒对理化因素的耐受力因毒株而异,但与病毒的毒力无关。因此可利用不利于病毒存活的理化因素如高温或化学品进行消毒,减少禽舍或环境中新城疫病毒的存在,阻断病毒传播。
[Abstract]:Objective to investigate the effect of water environment on the infectious ability of Newcastle disease virus (NDV) strains in China, and the difference in tolerance between Newcastle disease virus (NDV) and weak Newcastle disease virus (NDV). Methods three NDV virulent strains and three attenuated strains were used to determine the dynamic changes of EID50 of chicken embryo under different temperature (200.37 鈩，
本文编号：2139141
[Abstract]:Objective to investigate the effect of water environment on the infectious ability of Newcastle disease virus (NDV) strains in China, and the difference in tolerance between Newcastle disease virus (NDV) and weak Newcastle disease virus (NDV). Methods three NDV virulent strains and three attenuated strains were used to determine the dynamic changes of EID50 of chicken embryo under different temperature (200.37 鈩，
本文编号：2139141
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