甘草黄酮对乙醇氧化损伤小鼠脑及肝肺组织形态的影响
发布时间：2018-07-23 15:08
【摘要】：为了观察甘草黄酮对氧化损伤小鼠脑、肝脏、肺脏组织形态的影响,试验把60只小鼠随机分成6组,每组10只,即空白对照组、模型组、维生素C组、甘草黄酮低剂量组、甘草黄酮中剂量组、甘草黄酮高剂量组,除空白对照组外其余各组连续采用甘草黄酮灌胃小鼠42 d,再灌胃乙醇建立乙醇损伤模型,观察不同浓度的甘草黄酮(40,100,150 mg/kg)灌服小鼠后试验现象,并处死小鼠后快速取出全脑、肝脏、肺脏进行H.E.染色,观察组织形态。结果表明:模型组小鼠脑梗死区域可见大量神经元变性、坏死,胞体缩小变形,核固缩,噬神经元现象,脑组织水肿。与模型组相比,甘草黄酮低剂量组小鼠病理改变的严重程度降低,但可见小量神经元变性坏死;甘草黄酮中剂量组小鼠病理改变较模型组轻,神经元变性坏死部分的数量减少,噬神经元现象也明显降低,脑组织水肿明显降低;甘草黄酮高剂量组小鼠梗死区亦可见少量的神经元变性、坏死,见不到"噬神经元现象",脑组织无水肿。甘草黄酮3个剂量组与乙醇氧化损伤模型相比较组织形态明显有差异。说明甘草黄酮对小鼠脑、肝脏、肺脏组织病理学改变具有明显的改善作用,且都呈现明显的剂量依赖效应。
[Abstract]:In order to observe the effects of Glycyrrhiza flavonoids on the brain, liver and lung tissue of mice with oxidative damage, 60 mice were randomly divided into 6 groups, with 10 rats in each group, namely, blank control group, model group, vitamin C group, low dose group of Glycyrrhiza flavonoids, dose group of Glycyrrhiza flavone, high dose of Glycyrrhiza flavone, and other groups in addition to blank control group. The flavonoid was perfused to 42 d in mice, then ethanol was perfused to establish a model of ethanol damage, and the effects of different concentrations of glycyrrhizin (40100150 mg/kg) on the mice were observed. After the mice were killed, the whole brain, liver and lungs were taken out by H.E. staining, and the tissue morphology was observed. The results showed that a large number of neurons in the cerebral infarction area of the model group could be seen in a large number of neuron changes. Compared with the model group, the severity of the pathological changes in the low dose group of Glycyrrhiza flavonoids decreased, but the small amount of neurons were degenerative and necrotic, and the dose group of Glycyrrhiza flavanone was lighter than the model group, and the number of neuron degeneration and necrosis was reduced. The neuron phenomenon was obviously decreased and the edema of brain tissue decreased obviously, and a small amount of neuron degeneration and necrosis were found in the high dose group of Glycyrrhiza flavanone group, and there was no edema in the brain tissue. The 3 dose groups of Glycyrrhiza flavonoids were significantly different from the ethanol oxidative damage model. Histopathological changes in brain, liver and lungs of mice were significantly improved, and both showed a dose-dependent effect.
【作者单位】： 新疆生产建设兵团塔里木畜牧科技重点实验室;塔里木大学动物科学学院;
【基金】：大学生创新创业训练计划项目(2014029)
【分类号】：S853.7
本文编号：2139781
[Abstract]:In order to observe the effects of Glycyrrhiza flavonoids on the brain, liver and lung tissue of mice with oxidative damage, 60 mice were randomly divided into 6 groups, with 10 rats in each group, namely, blank control group, model group, vitamin C group, low dose group of Glycyrrhiza flavonoids, dose group of Glycyrrhiza flavone, high dose of Glycyrrhiza flavone, and other groups in addition to blank control group. The flavonoid was perfused to 42 d in mice, then ethanol was perfused to establish a model of ethanol damage, and the effects of different concentrations of glycyrrhizin (40100150 mg/kg) on the mice were observed. After the mice were killed, the whole brain, liver and lungs were taken out by H.E. staining, and the tissue morphology was observed. The results showed that a large number of neurons in the cerebral infarction area of the model group could be seen in a large number of neuron changes. Compared with the model group, the severity of the pathological changes in the low dose group of Glycyrrhiza flavonoids decreased, but the small amount of neurons were degenerative and necrotic, and the dose group of Glycyrrhiza flavanone was lighter than the model group, and the number of neuron degeneration and necrosis was reduced. The neuron phenomenon was obviously decreased and the edema of brain tissue decreased obviously, and a small amount of neuron degeneration and necrosis were found in the high dose group of Glycyrrhiza flavanone group, and there was no edema in the brain tissue. The 3 dose groups of Glycyrrhiza flavonoids were significantly different from the ethanol oxidative damage model. Histopathological changes in brain, liver and lungs of mice were significantly improved, and both showed a dose-dependent effect.
【作者单位】： 新疆生产建设兵团塔里木畜牧科技重点实验室;塔里木大学动物科学学院;
【基金】：大学生创新创业训练计划项目(2014029)
【分类号】：S853.7
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