牦牛角性状候选基因的筛选

发布时间：2018-07-25 07:03

【摘要】：本研究旨在筛选牦牛角性状候选基因,为角发育机制的研究和无角牦牛的培育提供科学依据。采集牦牛角发育初期胎龄90d的有角和无角牛的角基及额部皮肤组织,利用实时荧光定量PCR技术(qRT-PCR),比较牛角性状候选基因OLIG1、OLIG2、IFNAR1、IFNAR2、C1 H21orf62、GCFC1、IFNGR2、SYNJ1、IL10RB、GART、RXFP2、FOXL2、TWIST1、TWIST2、ZEB2、E-cadherin和P-cadherin mRNA在角基间及皮肤间的相对表达量。结果显示,候选基因OLIG1、IFNAR1、C1 H21orf62、SYNJ1、IL10RB、GART、RXFP2、TWIST2和E-cadherin在有角和无角牦牛角基间和皮肤间转录量差异不显著(P0.05)。IFNGR2、FOXL2、TWIST1和P-cadherin在有角和无角牦牛角基间转录量差异显著(P0.05)。IFNAR2和GCFC1在有角和无角牦牛角基间和皮肤间转录量差异都显著(P0.05)。OLIG2和ZEB2在有角和无角牦牛角基间转录量差异极显著(P0.01)。推测OLIG2和FOXL2可能是牛角性状的关键候选基因,同时ZEB2、TWIST1和IFNGR2基因可能对牛角的形成具有重要作用,而P-cadherin基因可能参与了牛角的发育。
[Abstract]:The purpose of this study was to screen candidate genes for yak horn traits and provide scientific basis for the study of angular development mechanism and the cultivation of horned yaks. The angular base and forehead skin tissues of horned and horned cattle were collected at the early stage of yak angle development 90d, and the real-time fluorescent quantitative PCR technique (qRT-PCR) was used to compare OLIG1, OLIG2, IFNAR1, IFNAR2, C1 H21orf62, GCFC1, IFNGR2, SYNJ1, IL10RB, GART, RXFP2, FOXL2, TWIST1, TWIST2, etc. The difference of P0.05.IFNGR2, FOXL2, TWIST1 and P-cadherin in the angular and non angular yak horns was significant (P0.05) between.IFNAR2 and GCFC1 in the angular and non angular yak horns and between the skin, and the difference in the transcriptional amount between the yak horns and the skin was significant (P0.05).OLIG2 and ZEB2 (P0.01) between the horns and the horned yak horns (P0.01). FOXL2 may be a key candidate gene for horns, and ZEB2, TWIST1 and IFNGR2 genes may play an important role in the formation of horns, and the P-cadherin gene may be involved in the development of horns.
【作者单位】：中国农业科学院兰州畜牧与兽药研究所;甘肃省牦牛繁育工程重点实验室;
【基金】：牦牛遗传资源与育种(CAAS-ASTIP-2014-LIHPS-01) 科技部科技支撑计划(2012BAD13B05)
【分类号】：S823.85

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