小鼠模型中研究复方中草药抗弓形虫感染的作用（英文）

发布时间：2018-07-25 18:11

【摘要】：目的:评估复方中草药对小鼠抗弓形虫的保护力,以期找到新型防治弓形虫病的药物。创新点:首次在小鼠模型中研究威灵仙、芜荑、白术、刘寄奴和甘草组成的复方中草药的抗弓形虫功效,并发现500 mg的药物能提供较好的保护力。方法:将ICR雌性小鼠随机分为7组:空白组不攻虫不给药;PBS组攻虫;药物对照组攻虫并口服磺胺嘧啶10 mg/d;实验组攻虫并分别灌胃300、400、500和600 mg/d中药煎剂;攻虫组每只小鼠腹腔注射弓形虫RH株1000个。每日观察各组小鼠死亡情况,在感染后3和7 d采集抗凝血检测血常规,并采集血液、肝、脾和肺组织,提取基因组DNA,通过绝对荧光定量PCR检测各组织样品中的虫荷量,采集感染后7 d的肝、肺和脾组织,制作组织切片,通过HE染色观察组织病变情况。结论:试验结果表明,500 mg中药组及磺胺嘧啶药物对照组的小鼠显著地延长了存活时间(图1),且这两组小鼠组织及血液中的虫荷数显著低于其他组(图2),这与小鼠的肝、肺和脾组织病理染色结果一致(图4)。另外,与300、400和600 mg中药处理组相比,500 mg处理组小鼠的单核细胞和淋巴细胞比例均较低(图3),这在一定程度上阻碍了弓形虫在血细胞中的传播。综上所述,500 mg中草药处理组能降低弓形虫的胞内增殖速率,延长小鼠的存活时间,证明该浓度的复方中药具有一定的抗弓形虫效果。
[Abstract]:Objective: to evaluate the protective effect of compound Chinese herbal medicine on Toxoplasma gondii in mice. Innovation: for the first time, the anti-toxoplasmosis effect of the compound Chinese herbal medicine composed of Weilingxian, catkin, Atractylodes macrocephala, Liu Yenu and Glycyrrhiza uralensis was studied in mice model for the first time, and it was found that 500 mg medicine could provide better protection. Methods: the female ICR mice were randomly divided into 7 groups: the blank group did not attack insects, the control group took 10 mg / d sulfadiazine, the control group was treated with 300400500 and 600 mg/d Chinese medicine decoction, and the control group was treated by oral administration of sulfadiazine 10 mg / d. 1 000 RH strains of Toxoplasma gondii were injected intraperitoneally to each mouse in attack group. The death rate of mice in each group was observed daily. Anticoagulant blood samples were collected 3 and 7 days after infection to detect blood routine, blood, liver, spleen and lung tissues were collected, genomic DNA was extracted, and the amount of insect load in tissue samples was detected by absolute fluorescence quantitative PCR. The liver, lung and spleen tissues were collected 7 days after infection, and the pathological changes were observed by HE staining. Conclusion: the results showed that the survival time was significantly prolonged in mice treated with 500 mg Chinese medicine and sulfadiazine drug control group (Fig. 1), and the number of worms in tissues and blood of these two groups was significantly lower than that in other groups (Fig. 2), which was associated with the liver of mice. The pathological staining of lung and spleen was consistent (Fig. 4). In addition, the proportion of monocytes and lymphocytes of mice treated with 300400 mg and 600mg Chinese medicine was lower than that of treated mice (Fig. 3), which hindered the transmission of Toxoplasma gondii in blood cells to some extent. In conclusion, the treatment group of 500 mg Chinese herbal medicine can reduce the intracellular proliferation rate of Toxoplasma gondii, prolong the survival time of mice, and prove that the Chinese herbal medicine of this concentration has a certain anti-Toxoplasma effect.
【作者单位】： Institute
【基金】：supported by the Science and Technology Department of Zhejiang Province(No.2012C12009-2),China
【分类号】：S853.74

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