日粮不同NDF水平对杂交水牛瘤胃细菌群落结构与瘤胃主要功能细菌的影响
发布时间：2018-07-26 14:19
【摘要】：本论文利用变性梯度凝胶电泳(DGGE)技术和实时荧光定量(q PCR)比较分析了饲喂不同中性洗涤纤维(NDF)水平日粮杂交水牛瘤胃细菌的多样性和数量差异,试验设计和结果如下:一、杂交水牛瘤胃微生物的多样性:为了研究杂交水牛瘤胃内与水牛耐粗饲相关的菌群,本试验改变日粮的营养组分,设计了3种不同日粮NDF水平(38%、44%、51%)。同时选择3头体况相近、健康、装有永久性瘤胃瘘管的杂交水牛,采用3×3拉丁方试验设计,试验分三期,每一期饲喂21天,最后一天采集瘤胃内容物,实验室提取总DNA,利用DGGE分析瘤胃液相与固相细菌群落结构。结果表明,(1)不同NDF水平日粮条件下,杂交水牛瘤胃固相与液相细菌群落区系都显示出了一定的差异性,但差异性并不显著。(2)在不同NDF水平日粮条件下,拟杆菌门与厚壁菌门均为瘤胃内的优势菌群。(3)在杂交水牛瘤胃液相细菌区系中,当NDF水平为44%时,瘤胃内瘤胃球菌属(Ruminococciis sp.)、解纤维热酸菌(Acidothermus cellulolyticus)、解纤维梭菌(Clostridium cellulolyticum)与另两组存在差异。在杂交水牛瘤胃固相细菌区系中,当NDF水平为38%时,普雷沃氏菌科(Prevotellaceae)较另外两组有差异。二、不同NDF水平日粮时杂交水牛瘤胃主要功能菌群的数量变化:通过Q-PCR的方法,分析了饲喂不同NDF水平日粮时杂交水牛瘤胃主要功能菌群的数量变化情况。结果表明:不同NDF水平日粮条件下,各组总普雷沃氏菌数量未产生显著性差异,但普雷沃氏菌属细菌数量在总细菌数量中占优势地位。反刍兽新月行单胞菌受到日粮NDF水平的影响,当NDF水平为44%时,数量最多。纤维降解菌产琥珀酸丝状杆菌、白色瘤胃球菌和黄色瘤胃球菌数量在各NDF水平下未产生显著性差异,但高NDF水平组数量高于另外两组,可能是由于日粮差异不够显著。不同NDF水平下脂解厌氧弧菌数量未受到影响。
[Abstract]:In this paper, denaturing gradient gel electrophoresis (DGGE) technique and real-time fluorescence quantitative (q PCR) (q PCR) were used to compare and analyze the diversity and quantity of rumen bacteria in buffalo fed with different neutral detergent fiber (NDF) levels. The results were as follows: 1. Diversity of rumen microbes in crossbred buffalo: in order to study the microflora in the rumen of crossbred buffalo, the nutrient components of diet were changed and three different dietary NDF levels (3844451%) were designed. At the same time, three crossbred buffaloes with similar body condition, healthy and permanent rumen fistula were selected. The experiment was designed by 3 脳 3 Latin square, and the experiment was divided into three stages, each of which was fed for 21 days, and the rumen contents were collected on the last day. Total DNA was extracted from the laboratory, and the structure of liquid and solid bacteria community in rumen was analyzed by DGGE. The results showed that: (1) under different NDF levels, the flora of rumen solid phase and liquid phase bacteria of crossbred buffalo showed some differences, but the differences were not significant. (2) under different NDF levels, the flora of bacteria was different. Bacteroides phylum and musculum thuringiensis were the dominant bacteria in rumen. (3) in the rumen liquid flora of hybrid buffalo, when the NDF level was 44, there was a difference between the two groups in the rumen bacteria, (Ruminococciis sp.), (Ruminococciis sp.), Clostridium cellulolyticum, (Acidothermus cellulolyticus), Clostridium fibrillolyticus, (Clostridium cellulolyticum). When the NDF level was 38, the (Prevotellaceae) of Prevoidae was different from that of the other two groups in the rumen solid phase flora of crossbred buffalo. Secondly, the quantity change of main functional flora in rumen of crossbred buffalo with different NDF level: the quantity change of main functional flora in rumen of crossbred buffalo fed with different NDF level was analyzed by Q-PCR method. The results showed that there was no significant difference in the number of Prevobacter in different diets with different NDF levels, but the number of Prevoia bacteria was dominant in the total number of bacteria. Ruminants were affected by dietary NDF level, and when NDF level was 444.The quantity was the most. There was no significant difference in the number of filamentous succinate bacteria, S. albicans and S. xanthoxylum produced by fibrinolytic bacteria, but the number of high NDF level group was higher than that of the other two groups, which may be due to the lack of significant difference in diet. The number of Vibrio acidolyticus at different NDF levels was not affected.
【学位授予单位】：华中农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S823.5
本文编号：2146288
[Abstract]:In this paper, denaturing gradient gel electrophoresis (DGGE) technique and real-time fluorescence quantitative (q PCR) (q PCR) were used to compare and analyze the diversity and quantity of rumen bacteria in buffalo fed with different neutral detergent fiber (NDF) levels. The results were as follows: 1. Diversity of rumen microbes in crossbred buffalo: in order to study the microflora in the rumen of crossbred buffalo, the nutrient components of diet were changed and three different dietary NDF levels (3844451%) were designed. At the same time, three crossbred buffaloes with similar body condition, healthy and permanent rumen fistula were selected. The experiment was designed by 3 脳 3 Latin square, and the experiment was divided into three stages, each of which was fed for 21 days, and the rumen contents were collected on the last day. Total DNA was extracted from the laboratory, and the structure of liquid and solid bacteria community in rumen was analyzed by DGGE. The results showed that: (1) under different NDF levels, the flora of rumen solid phase and liquid phase bacteria of crossbred buffalo showed some differences, but the differences were not significant. (2) under different NDF levels, the flora of bacteria was different. Bacteroides phylum and musculum thuringiensis were the dominant bacteria in rumen. (3) in the rumen liquid flora of hybrid buffalo, when the NDF level was 44, there was a difference between the two groups in the rumen bacteria, (Ruminococciis sp.), (Ruminococciis sp.), Clostridium cellulolyticum, (Acidothermus cellulolyticus), Clostridium fibrillolyticus, (Clostridium cellulolyticum). When the NDF level was 38, the (Prevotellaceae) of Prevoidae was different from that of the other two groups in the rumen solid phase flora of crossbred buffalo. Secondly, the quantity change of main functional flora in rumen of crossbred buffalo with different NDF level: the quantity change of main functional flora in rumen of crossbred buffalo fed with different NDF level was analyzed by Q-PCR method. The results showed that there was no significant difference in the number of Prevobacter in different diets with different NDF levels, but the number of Prevoia bacteria was dominant in the total number of bacteria. Ruminants were affected by dietary NDF level, and when NDF level was 444.The quantity was the most. There was no significant difference in the number of filamentous succinate bacteria, S. albicans and S. xanthoxylum produced by fibrinolytic bacteria, but the number of high NDF level group was higher than that of the other two groups, which may be due to the lack of significant difference in diet. The number of Vibrio acidolyticus at different NDF levels was not affected.
【学位授予单位】：华中农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S823.5
【参考文献】
相关期刊论文 前2条
1 刘利;唐纪良;冯家勋;;广西水牛瘤胃中的细菌多样性[J];微生物学报;2009年02期
2 冯薇;王加启;刘开朗;卜登攀;李旦;赵圣国;杨开伦;;运用PCR-DGGE分析比较瘤胃中不同饲料固相粘附微生物区系[J];畜牧兽医学报;2010年12期
，本文编号：2146288
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