我国部分地区猪沙门菌的耐药性

发布时间：2018-07-27 20:53

【摘要】：以2014-2015年从我国部分省市规模化养殖场中分离的55株沙门菌为对象,选取临床常用的12种抗菌药,用微量肉汤稀释法检测所分离的沙门菌的耐药性。结果显示:测试的12种药物中沙门菌对甲氧苄啶最敏感,耐药率只有1.82%,除此之外对其他11种药物的耐药率均在50%以上,而且对其中的氨苄西林、氟苯尼考、多西环素和四环素等4大类共计7种药物的耐药率在80%以上;在多重耐药性分析方面,1株沙门菌对所有药物完全敏感,另外54株菌的药物耐受数量在5种到11种之间,其中耐受10种药物是所有沙门菌中最大的一类菌株,占总数的30.91%。根据菌株的耐药特点,设计了23对包含各类药物主要耐药基因的引物,用PCR法检测相应耐药菌株对该耐药基因的携带情况,一共检测出了15种耐药基因,其中检出率最高的几种基因分别是sul2基因(检出率78.43%)、sul1基因(检出率60.78%)和tet A基因(检出率72.55%)等。此外,对于耐受喹诺酮类药物的菌株还检测了其耐药决定区域基因gyrA和parC所编码氨基酸的突变情况。测序结果表明,gyrA基因所编码氨基酸序列的第83个位点和第87个位点发生了突变,分别由酪氨酸变成了丝氨酸或亮氨酸、苯丙氨酸,由天冬氨酸变成了天冬酰胺或酪氨酸,parC基因所编码氨基酸序列没有发现突变的情况。
[Abstract]:Taking 55 strains of Salmonella isolated from large-scale farms in some provinces and cities in China in 2014-2015, 12 kinds of antimicrobial agents commonly used in clinic were selected, and the drug resistance of the isolated Salmonella was detected by the method of broth dilution. The results showed that Salmonella was the most sensitive to trimethoprim, and the resistance rate was only 1.82%. In addition, the resistance rates to the other 11 drugs were all above 50%, and ampicillin and florfenicol were the most sensitive to trimethoprim and florfenicol. The drug resistance rate of 7 drugs in 4 major categories, such as doxycycline and tetracycline, was more than 80%. In multidrug resistance analysis, one Salmonella strain was completely sensitive to all drugs, and the other 54 strains had drug resistance of between 5 and 11. Among them, 10 drugs were the largest of all Salmonella strains, accounting for 30.91% of the total. According to the drug resistance characteristics of the strain, 23 pairs of primers containing the major drug resistance genes were designed, and the carrying status of the drug resistance genes was detected by PCR method. A total of 15 drug resistance genes were detected. The highest detection rates were sul2 gene (78.43%), sul1 gene (60.78%) and tet A gene (72.55%). In addition, the mutations of amino acids encoded by gyrA and parC were also detected for the strains resistant to quinolones. The results of sequencing showed that the 83 and 87 sites of amino acid sequence encoded by gyrA gene were mutated from tyrosine to serine or leucine, phenylalanine, respectively. From aspartic acid to asparagine or tyrosine parC gene, no mutation was found in the amino acid sequence.
【作者单位】：华中农业大学动物医学院/农业微生物学国家重点实验室/生猪健康养殖协同创新中心;
【基金】：公益性行业农业科研专项资助项目(201403054) 湖北省“科技支撑计划”资助项目(2013BBB14,2014BBB10)
【分类号】：S852.61

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