西镇牛微卫星DNA与生长发育性状的关联性分析

发布时间：2018-07-29 06:20

【摘要】：【目的】分析12个微卫星DNA与西镇牛长发育性状的关联性,为西镇牛品种资源保护和开发利用提供理论依据。【方法】测定西镇牛的体高、体长、胸围、管围、腰角宽和体质量。利用12对微卫星引物,采用PCR扩增和非变性聚丙稀酰胺凝胶电泳技术,对48头西镇牛母牛进行了遗传多样性检测,并统计分析各位点各种基因型群体与体尺性状之间的效应关系。【结果】12个微卫星位点各基因型与西镇牛生长发育性状具有关联性。IDVGA2、BM2113、DVGA55、ETH225、HEL9、ILSTS005、BM1824位点与体高,BM2113、DVGA55、ETH225、HEL9、ILSTS005、ETH10、DAVG44位点与体长,IDVGA2、DVGA55、HEL9、ILST093、IDVGA27、BM1824位点与胸围,IDVGA2、ETH225、DAVG44位点与管围,BM2113、DVGA55、ETH225、HEL9、BM1905、ILSTS005、ILST093、ETH10位点与腰角宽,IDVGA2、BM2113、DVGA55、ETH225、HEL9、ILST093、BM1824位点与体质量具有显著或极显著的效应关系。【结论】筛选出了对西镇牛生长发育性状具有正效应的等位基因45个,具有负效应的等位基因40个,可用于指导西镇牛育种实践。
[Abstract]:[objective] to analyze the correlation between 12 microsatellite DNA and the growth traits of Xizhen cattle, and to provide a theoretical basis for the conservation, exploitation and utilization of the cattle breed resources. [methods] the body height, body length, chest circumference and tube circumference of Xizhen cattle were determined. Waist width and body mass. Using 12 pairs of microsatellite primers, PCR amplification and non-denatured polyacrylamide gel electrophoresis were used to detect the genetic diversity of 48 cattle cows in Xizhen. [results] 12 microsatellite loci were correlated with the growth and development traits of Xizhen cattle. IDVGA 2, BM2113, DVGA5, ETH225, HEL9, ILSTS005, BM1824 and height BM2113DVGA5ETS005, ETH10DAVG44 and body height BM2113DVGA5ETH225, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44, ETH10DAVG44 and ETH10DAVG44. ,IDVGA2,DVGA55,HEL9,ILST093,IDVGA27,BM1824浣嶇偣涓庤兏鍥，

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