公鸡鸡冠发育相关候选基因的表达分析

发布时间：2018-07-29 17:23

【摘要】：为了探索鸡冠发育的分子机制,利用实时荧光定量PCR技术(qRT-PCR)对前期采用转录组测序技术筛选出的7个与鸡冠发育相关的候选基因(BMP2、TPPP3、HSD17B2、CYP2W1、CYP1A4、EDA2R、HAL)进行表达分析。选取200只5周龄青脚麻鸡A系公鸡,从中筛选出15只高鸡冠和15只矮鸡冠公鸡,采用qRT-PCR定量分析7个候选基因在两组个体鸡冠组织的相对表达量,同时检测了7个候选基因在心、肝等11个组织的表达谱。结果显示:CYP2W1和TPPP3在高冠组表达量极显著高于矮冠组(P0.01);CYP4A1和EDA2R在高冠组表达量高于矮冠组(P0.05);HAL在高冠组中表达量极显著低于矮冠组(P0.01);HSD17B2和BMP2在高冠组中表达量显著低于矮冠组(P0.05)。表明BMP2、TPPP3、CYP2W1、EDA2R四个基因在鸡冠中的表达属于较高水平表达,其余基因在鸡冠组织中属于中等水平表达。提示这7个候选基因可能在调控鸡冠发育中发挥重要作用。
[Abstract]:In order to explore the molecular mechanism of the development of chicken crowns, seven candidate genes (BMP2, TPPP3, HSD17B2CYP2W1, CYP1A4, EDA2RHAL), which were screened by transcriptome sequencing technique, were analyzed by real-time fluorescence quantitative PCR (qRT-PCR). Two hundred 5-week-old A-line roosters were selected and 15 tall and 15 dwarf crowns were selected. The relative expression of 7 candidate genes in the two groups was quantitatively analyzed by qRT-PCR. At the same time, the expression profiles of 7 candidate genes in heart, liver and other 11 tissues were detected. The results showed that the expression of CYP2W1 and TPPP3 in the high crown group was significantly higher than that in the low crown group (P0.01), and the expression of CYP4A1 and EDA2R in the high crown group was significantly higher than that in the low crown group (P0.05). The expression of HSD17B2 and BMP2 in the high crown group was significantly lower than that in the low crown group (P0.05). The results showed that the expression of BMP2TPPP3CYP2W1 and EDA2R was high in the crowns, while the other genes were moderately expressed in the crowns. These 7 candidate genes may play an important role in the regulation of the development of chicken crowns.
【作者单位】：南京农业大学;江苏省家禽科学研究所;
【基金】：现代农业产业技术体系建设专项资金(CARS-41) 江苏省农业三新工程项目(SXGC[2017]254) 江苏省农业自主创新资金(CX(15)1009) 江苏省重点研发计划(现代农业)重点项目(BE2015344) 扬州市科技公共服务平台建设(YZ2016221)
【分类号】：S831

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