母猪妊娠期日粮添加甲基供体或双酚A对后代肠道发育及功能的影响
发布时间：2018-08-01 14:31
【摘要】：母体营养可以通过表观遗传修饰途径例如改变DNA甲基化水平,持续影响后代生长发育。本试验以能够诱导基因发生DNA低甲基化的双酚A (Bisphenol A,BPA)作为负对照,研究母猪妊娠期日粮添加甲基供体(Methyl donor, MET)对后代肠道发育及功能的影响,并在此基础上考察了甲基供体是否能抵消双酚A对肠道发育及功能的作用。选择体重(221.14±2.57kg)、胎次(3-5)接近的60头LY母猪,配种后随机分为4组(n=15),妊娠期各组分别提供日粮如下：1)CON组,基础日粮;2)BPA组,基础日粮+双酚A；3)MET组,基础日粮+甲基供体；4) MET+BPA组,基础日粮+甲基供体+双酚A。试验母猪从妊娠第一天开始供给相应试验日粮,直至分娩。分娩后所有母猪自由采食同一饲粮,28天断奶。分娩时每组选择12头分别来自不同母猪并且接近窝平均体重的仔猪进行屠宰取样；断奶时以同样的选猪原则从每组中选择6头仔猪进行屠宰取样。试验结果如下：1、母猪妊娠期日粮添加甲基供体对新生仔猪平均体重与出生窝重无显著影响,但显著增加了断奶仔猪平均体重及断奶窝重(P0.05)。双酚A显著增加了新生仔猪平均体重及出生窝重(P0.05),但断奶平均体重及断奶窝重未受到双酚A显著影响。对于活产仔数、新生仔猪平均体重与断奶仔猪平均体重,甲基供体与双酚A有显著的互作效应(P0.05)。2、母猪妊娠期日粮添加甲基供体显著降低了新生与断奶仔猪单位体重小肠长度(P0.05),但显著增加了单位长度小肠重量(P0.05)。双酚A的添加有降低新生仔猪单位体重小肠长度的趋势(P=0.06)。3、母猪妊娠期日粮添加甲基供体显著增加了新生和断奶仔猪空肠与回肠的绒毛高度/隐窝深度(P0.05)。双酚A显著降低了新生和断奶仔猪空肠绒毛高度/隐窝深度(P0.05)。同时,对于新生仔猪空肠和断奶仔猪回肠绒毛高度/隐窝深度,甲基供体与双酚A有显著的互作效应(P0.05)。4、母猪妊娠期日粮添加甲基供体显著增加了新生和断奶仔猪空肠乳糖酶的活性(P0.05),同时显著增加断奶仔猪十二指肠和空肠蔗糖酶活性(P0.05),并且显著上调了新生与断奶仔猪空肠乳糖酶的基因表达(P0.05)。双酚A显著降低了新生仔猪空肠蔗糖酶活性(P0.05),对断奶仔猪空肠蔗糖酶活性有降低的趋势(P=0.07)。对于新生与断奶仔猪空肠乳糖酶活性,甲基供体与双酚A有显著的互作效应(P0.05)。5、母猪妊娠期日粮添加甲基供体显著上调了新生与断奶仔猪空肠Pept1和Sglt1基因的相对表达量(P0.05)。双酚A显著下调了新生和断奶仔猪空肠Pept1基因的相对表达量(P0.05)。同时,与BPA组相比,MET+BPA组新生与断奶仔猪空肠Pept1基因的相对表达量显著增加(P0.05)。6、母猪妊娠期日粮添加甲基供体显著增加了新生仔猪空肠Pept1基因启动子的DNA甲基化水平(P0.05),双酚A显著降低了新生仔猪空肠Pept1基因启动子的DNA甲基化水平(P0.05),MET+BPA组与CON组无显著差异。7、母猪妊娠期日粮添加甲基供体显著增加新生仔猪空肠DNMT1、DNMT3a和MTHFR基因的相对表达量(P0.05)。双酚A显著降低了新生仔猪空肠DNMT3a基因的相对表达量(P0.05)。所有甲基化相关酶的基因表达在MET+BPA组与CON组均无显著差异。综上所述,母猪妊娠期日粮添加甲基供体能通过改善新生与断奶仔猪肠道形态,上调肠道酶活与营养物质转运载体基因表达进而提高肠道的消化吸收功能促进仔猪的生长发育。相对而言母猪妊娠期日粮添加双酚A损伤了后代肠道发育及功能,但甲基供体能够抵消双酚A对肠道的损伤进而改善后代生长发育。
[Abstract]:Maternal nutrition can affect the growth and development of offspring through epigenetic modification, such as DNA methylation, which can continue to affect the growth and development of offspring. In this experiment, the diphenol A (Bisphenol A, BPA), which can induce DNA hypomethylation, was used as a negative control to study the intestinal development and function of the offspring of the sow during pregnancy (Methyl donor, MET). On this basis, we examined whether the methyl donor could counteract the effect of bisphenol A on the intestinal development and function. Select 60 LY sows with weight (221.14 + 2.57kg) and parity (3-5), and randomly divide them into 4 groups (n=15). Each group of pregnancy provides diet as follows: 1) CON, basal diet; 2) BPA, basal diet + bisphenol A; 3) M ET group, basal diet + methyl donor, 4) MET+BPA group, basal diet + methyl donor + bisphenol A. test sow from the first day of pregnancy to supply the corresponding experimental diet until delivery. After childbirth, all sows were free to feed the same diet and 28 days of weaning. Each group selected 12 of each group from different sows and close to the average weight of the nest. Pigs were slaughtered and sampled, and 6 piglets were selected from each group for slaughter sampling with the same pig selection principle during weaning. The results were as follows: 1, the average weight and litter weight of newborn piglets had no significant effect on the average weight and litter weight of newborn piglets in the sow pregnancy diet, but the average weight and weaned litter weight (P0.05) of the weaned piglets increased significantly. 1 The average weight and litter weight of newborn piglets (P0.05) were increased, but the average weaning weight and weaning litter weight were not significantly affected by bisphenol A. For the number of live births, the average weight of the newborn piglets, the average weight of the weaned piglets, the methyl donor and the bisphenol A had a significant interaction effect (P0.05).2, and the sow diet supplemented with methyl donor was significantly reduced. The unit weight small intestine length (P0.05) was lower in newborn and weanling piglets, but the small intestine weight per unit length (P0.05) was significantly increased. The addition of bisphenol A decreased the small intestine length of newborn piglets (P=0.06).3. The addition of methyl donor in sow pregnancy diet increased the height / hidden height of the jejunum and ileum in newborn and weanling piglets. Pit depth (P0.05). Bisphenol A significantly reduced the height of jejunum villus / recess depth (P0.05) in newborn and weanling piglets. At the same time, there was a significant interaction effect (P0.05) between methyl donor and bisphenol A (P0.05).4 for newborn piglet jejunum and Weanling Piglet's ileum height / recess depth. The activity of jejunum lactase (P0.05) in weanling piglets significantly increased the activity of sucrase (P0.05) in the duodenum and jejunum of weaned piglets, and significantly increased the gene expression of jejunum lactase (P0.05) in newborn and weanling piglets. Bisphenol A significantly reduced the activity of Sucrose Enzyme in newborn piglets (P0.05), and the activity of sucrase in the jejunum of weanling piglets had a significant effect on sucrase activity in weanling piglets. Decreasing trend (P=0.07). For newborn and weaned piglets, the activity of the jejunum lactase, methyl donor and bisphenol A have significant interaction effect (P0.05).5. The addition of methyl donor in sow pregnancy diet significantly up-regulated the relative apparent amount of Pept1 and Sglt1 genes between newborn and weaned piglets (P0.05). Bisphenol A significantly downgraded newborn and weanling piglets. The relative expression of the jejunum Pept1 gene (P0.05). At the same time, compared with the BPA group, the relative expression of the Pept1 gene of the jejunum in the MET+BPA group and the Weanling Piglet increased significantly (P0.05).6. The addition of methyl donor in the sow pregnancy diet significantly increased the DNA methylation level (P0.05) of the newborn piglet jejunum Pept1 gene promoter (P0.05), and the bisphenol A was significantly reduced. The DNA methylation level (P0.05) of the Pept1 gene promoter in newborn piglets was not significantly different from that in the CON group,.7. The relative expression of DNMT1, DNMT3a and MTHFR genes in newborn piglets was significantly increased by adding methyl donor in the sow gestation diet (P0.05). The relative expression of the jejunum DNMT3a gene in newborn piglets was significantly reduced. 05). The gene expression of all methylation related enzymes is not significantly different between the MET+BPA group and the CON group. To sum up, the addition of methyl donor in the sow pregnancy diet can improve the intestinal morphology of newborn and weanling piglets, up-regulation the intestinal enzyme activity and the gene expression of nutrient transport carrier and then improve the digestive and absorption function of the intestinal tract to promote the growth of piglets. Long development. Relatively speaking, the addition of bisphenol A in the diet of sow during pregnancy damage the intestinal development and function of offspring, but the methyl donor can counteract the damage of the bisphenol A to the intestine and then improve the growth and development of the offspring.
【学位授予单位】：四川农业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S828.5
本文编号：2157883
[Abstract]:Maternal nutrition can affect the growth and development of offspring through epigenetic modification, such as DNA methylation, which can continue to affect the growth and development of offspring. In this experiment, the diphenol A (Bisphenol A, BPA), which can induce DNA hypomethylation, was used as a negative control to study the intestinal development and function of the offspring of the sow during pregnancy (Methyl donor, MET). On this basis, we examined whether the methyl donor could counteract the effect of bisphenol A on the intestinal development and function. Select 60 LY sows with weight (221.14 + 2.57kg) and parity (3-5), and randomly divide them into 4 groups (n=15). Each group of pregnancy provides diet as follows: 1) CON, basal diet; 2) BPA, basal diet + bisphenol A; 3) M ET group, basal diet + methyl donor, 4) MET+BPA group, basal diet + methyl donor + bisphenol A. test sow from the first day of pregnancy to supply the corresponding experimental diet until delivery. After childbirth, all sows were free to feed the same diet and 28 days of weaning. Each group selected 12 of each group from different sows and close to the average weight of the nest. Pigs were slaughtered and sampled, and 6 piglets were selected from each group for slaughter sampling with the same pig selection principle during weaning. The results were as follows: 1, the average weight and litter weight of newborn piglets had no significant effect on the average weight and litter weight of newborn piglets in the sow pregnancy diet, but the average weight and weaned litter weight (P0.05) of the weaned piglets increased significantly. 1 The average weight and litter weight of newborn piglets (P0.05) were increased, but the average weaning weight and weaning litter weight were not significantly affected by bisphenol A. For the number of live births, the average weight of the newborn piglets, the average weight of the weaned piglets, the methyl donor and the bisphenol A had a significant interaction effect (P0.05).2, and the sow diet supplemented with methyl donor was significantly reduced. The unit weight small intestine length (P0.05) was lower in newborn and weanling piglets, but the small intestine weight per unit length (P0.05) was significantly increased. The addition of bisphenol A decreased the small intestine length of newborn piglets (P=0.06).3. The addition of methyl donor in sow pregnancy diet increased the height / hidden height of the jejunum and ileum in newborn and weanling piglets. Pit depth (P0.05). Bisphenol A significantly reduced the height of jejunum villus / recess depth (P0.05) in newborn and weanling piglets. At the same time, there was a significant interaction effect (P0.05) between methyl donor and bisphenol A (P0.05).4 for newborn piglet jejunum and Weanling Piglet's ileum height / recess depth. The activity of jejunum lactase (P0.05) in weanling piglets significantly increased the activity of sucrase (P0.05) in the duodenum and jejunum of weaned piglets, and significantly increased the gene expression of jejunum lactase (P0.05) in newborn and weanling piglets. Bisphenol A significantly reduced the activity of Sucrose Enzyme in newborn piglets (P0.05), and the activity of sucrase in the jejunum of weanling piglets had a significant effect on sucrase activity in weanling piglets. Decreasing trend (P=0.07). For newborn and weaned piglets, the activity of the jejunum lactase, methyl donor and bisphenol A have significant interaction effect (P0.05).5. The addition of methyl donor in sow pregnancy diet significantly up-regulated the relative apparent amount of Pept1 and Sglt1 genes between newborn and weaned piglets (P0.05). Bisphenol A significantly downgraded newborn and weanling piglets. The relative expression of the jejunum Pept1 gene (P0.05). At the same time, compared with the BPA group, the relative expression of the Pept1 gene of the jejunum in the MET+BPA group and the Weanling Piglet increased significantly (P0.05).6. The addition of methyl donor in the sow pregnancy diet significantly increased the DNA methylation level (P0.05) of the newborn piglet jejunum Pept1 gene promoter (P0.05), and the bisphenol A was significantly reduced. The DNA methylation level (P0.05) of the Pept1 gene promoter in newborn piglets was not significantly different from that in the CON group,.7. The relative expression of DNMT1, DNMT3a and MTHFR genes in newborn piglets was significantly increased by adding methyl donor in the sow gestation diet (P0.05). The relative expression of the jejunum DNMT3a gene in newborn piglets was significantly reduced. 05). The gene expression of all methylation related enzymes is not significantly different between the MET+BPA group and the CON group. To sum up, the addition of methyl donor in the sow pregnancy diet can improve the intestinal morphology of newborn and weanling piglets, up-regulation the intestinal enzyme activity and the gene expression of nutrient transport carrier and then improve the digestive and absorption function of the intestinal tract to promote the growth of piglets. Long development. Relatively speaking, the addition of bisphenol A in the diet of sow during pregnancy damage the intestinal development and function of offspring, but the methyl donor can counteract the damage of the bisphenol A to the intestine and then improve the growth and development of the offspring.
【学位授予单位】：四川农业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S828.5
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