马链球菌马亚种新疆分离株菌体蛋白的粗提取及免疫效果初步评估
发布时间：2018-08-03 16:24
【摘要】：马腺疫是由马链球菌马亚种引起的严重危害马属动物传染病之一,该病给马养殖业造成严重经济损失。可引起马高烧,下颌淋巴结肿大等症状。马链球菌马亚种属于Lancefield C型链球菌,该菌具有多种菌体蛋白,成为其重要的毒力因子和保护性抗原。本研究主要对马链球菌马亚种新疆分离株XZ1菌体蛋白的免疫原性与免疫保护力进行分析。本研究提取了马链球菌马亚种新疆分离株XZ1的菌体蛋白,利用SDS-PAGE方法检测提取的菌体蛋白,分别将提取的菌体蛋白与兔抗全菌的抗体及康复马血清反应,利用Western-blot和ELISA试验分析其免疫原性,二者的实验结果均表明提取的菌体蛋白具有良好的抗体识别能力且能够与康复马抗体反应的能力。为探索马链球菌马亚种新疆分离株提取的菌体蛋白免疫效果,本研究利用提取马链球菌马亚种菌体蛋白免疫小鼠并检测免疫后的抗体水平及免疫保护力。结果表明,提取的菌体蛋白免疫小鼠后,可诱导小鼠产生特异性抗体,免疫后的抗体效价高于灭活的全菌,对小鼠的免疫保护率可达到67%;表明该菌体蛋白具有良好的免疫保护效果。为进一步检测菌体蛋白对马的免疫效果,我们将提取马链球菌马亚种新疆分离株的菌体蛋白免疫马并检测免疫后抗体水平和细胞免疫水平。结果表明,该菌体蛋白能够诱导马产生特异性抗体并可诱导机体的细胞免疫应答,二免后抗体水平及细胞免疫水平同对照组相比差异显著(P0.01),该结果为进一步开发有效的马腺疫的防控方法提供了理论和实践基础。
[Abstract]:The equine adenosis is one of the serious infectious diseases of the genus equine caused by Maya species of Streptococcus equine, which causes serious economic losses to the horse breeding industry. Can cause equine high fever, mandibular lymph node enlargement and other symptoms. Streptococcus equi Maya belongs to Lancefield C Streptococcus. It has many bacterial proteins and has become an important virulence factor and protective antigen. In this study, the immunogenicity and immunological protection of XZ1 bacterial protein of Streptococcus equi Maya strain from Xinjiang were analyzed. In this study, the bacterial proteins of Streptococcus equina Maya strain XZ1 were extracted. The extracted bacterial proteins were detected by SDS-PAGE method, and reacted with rabbit anti-bacterial antibody and rehabilitative horse serum, respectively. Western-blot and ELISA tests were used to analyze their immunogenicity. The results of both experiments showed that the extracted bacterial protein had good ability of antibody recognition and could react with the antibody of rehabilitative horse. In order to investigate the immunological effect of Streptococcus equina Maya isolated from Xinjiang strain, this study was conducted to immunize mice with Streptococcus equina Maya strain bacterial protein and to detect the antibody level and immune protection ability after immunization. The results showed that the specific antibody was induced by immunizing mice with the extracted bacterial protein, and the titer of the antibody after immunization was higher than that of the inactivated whole bacteria. The immune protection rate of mice was 67%, which indicated that the bacterial protein had a good immune protection effect. In order to further test the immune effect of bacterial protein on horse, we will extract the bacterial protein from Streptococcus equine Maya strain Xinjiang to immunize the horse, and detect the antibody level and cellular immunity level after immunization. The results showed that the bacterial protein could induce the production of specific antibodies and the cellular immune response of the body. Compared with the control group, the level of antibody and cellular immunity after the second immunization were significantly different (P0.01). The results provided a theoretical and practical basis for the further development of effective methods for the prevention and control of equine adenosis.
【学位授予单位】：新疆农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S852.61
本文编号：2162308
[Abstract]:The equine adenosis is one of the serious infectious diseases of the genus equine caused by Maya species of Streptococcus equine, which causes serious economic losses to the horse breeding industry. Can cause equine high fever, mandibular lymph node enlargement and other symptoms. Streptococcus equi Maya belongs to Lancefield C Streptococcus. It has many bacterial proteins and has become an important virulence factor and protective antigen. In this study, the immunogenicity and immunological protection of XZ1 bacterial protein of Streptococcus equi Maya strain from Xinjiang were analyzed. In this study, the bacterial proteins of Streptococcus equina Maya strain XZ1 were extracted. The extracted bacterial proteins were detected by SDS-PAGE method, and reacted with rabbit anti-bacterial antibody and rehabilitative horse serum, respectively. Western-blot and ELISA tests were used to analyze their immunogenicity. The results of both experiments showed that the extracted bacterial protein had good ability of antibody recognition and could react with the antibody of rehabilitative horse. In order to investigate the immunological effect of Streptococcus equina Maya isolated from Xinjiang strain, this study was conducted to immunize mice with Streptococcus equina Maya strain bacterial protein and to detect the antibody level and immune protection ability after immunization. The results showed that the specific antibody was induced by immunizing mice with the extracted bacterial protein, and the titer of the antibody after immunization was higher than that of the inactivated whole bacteria. The immune protection rate of mice was 67%, which indicated that the bacterial protein had a good immune protection effect. In order to further test the immune effect of bacterial protein on horse, we will extract the bacterial protein from Streptococcus equine Maya strain Xinjiang to immunize the horse, and detect the antibody level and cellular immunity level after immunization. The results showed that the bacterial protein could induce the production of specific antibodies and the cellular immune response of the body. Compared with the control group, the level of antibody and cellular immunity after the second immunization were significantly different (P0.01). The results provided a theoretical and practical basis for the further development of effective methods for the prevention and control of equine adenosis.
【学位授予单位】：新疆农业大学
【学位级别】：硕士
【学位授予年份】：2015
【分类号】：S852.61
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