猫异源输入犬血的可行性研究及对猫急性失血性休克的疗效观察

发布时间：2018-08-06 10:49

【摘要】：输血疗法是一种输入健康动物的血液或血液成分来进行治疗的方法。机体血液或血液成分丢失时,输血疗法可以起到补血、止血、解毒的治疗效果,作为一种有效的抢救措施已经广泛应用在临床诊疗之中。异源输血是异种动物之间的输血,包括全血、血液成分和血液制品,临床上已有相关病例。本文通过对猫异源输入犬血的可行性研究及其对猫急性失血性休克的疗效观察,进而为其在宠物临床上应用提供指导。试验Ⅰ猫异源输入犬血的可行性试验研究本试验研究猫异源输入犬血的可行性。试验选取18只平均体重(4±0.5)kg的健康成年猫,随机分为3组,每组6只。采用耳标法对所有试验猫进行标记识别。分别设定为空白对照组A组(静脉输入生理盐水)、试验组B组(静脉输入猫全血)、试验组C组(静脉输入犬全血),输入量为受试动物总血量的20%。试验期间观察各组试验猫的临床表现,比较各组猫在试验前2h、试验后0.5 h、1h、2 h及连续7 d的血液学、血清生化、血清电解质、尿液等指标,评价猫异源输入犬血的可行性。结果表明:输入猫全血的B组、输入犬全血的C组与输入生理盐水的A组之间差异不显著(p0.05)。试验Ⅱ 猫急性失血性休克模型的建立本试验选取9只平均体重(4±0.5)kg的健康成年猫,采取自身前后对照方法,心脏放血建立猫急性失血性休克模型。具体方法:将各组试验猫镇静后按20 ml/kg进行心脏放血,并于试验期间观察试验猫临床症状,比较模型制备前30 min(T01)及放血完成后即刻(T0)、15 min(T15)、30 min(T30)四个时间点,试验猫的精神状态、体温、呼吸、心率、脉搏、可视粘膜颜色、饮欲、食欲、粪便以及尿液情况等。结果:共有9只猫成功建立急性失血性中度休克模型,各试验猫失血后出现体温下降、心率增快、可视黏膜颜色苍白、精神委靡、粪尿明显减少等症状;与模型制备前30 min(T01)相比,放血完成后30 min(T30),试验猫淋巴细胞、单核细胞、中性粒细胞、红细胞、血红蛋白、红细胞压积明显下降超出正常范围,且差异显著(P0.05),Na+、K+、Cl-含量明显下降(P0.05),血压明显降低(P0.05)。结果表明:以猫作为模型动物,采用心脏放血的方法可以确实、迅速的构建急性失血性休克模型。试验Ⅲ 猫异源输入犬血对猫急性失血性休克的疗效观察本试验为探究猫异源输入犬血对猫急性失血性休克的治疗效果,将试验Ⅱ中急性失血性休克建模成功的9只猫随机分为3组,分别设定为输液组(A)、输液+猫血组(B)、输液+犬血组(C)。通过观察输液前2h、输液完成后2h及第1、3、7、14 d各组试验猫的临床症状、血液学、血清生化、血清电解质及尿液等指标,评价猫异源输入犬血的临床疗效。结果表明:B、C组的各项指标均与A组有显著差异(p0.05),B、C组之间差异不显著(p0.05);试验期间输血组未出现与输血相关的不良反应。表明:猫异源输入犬血对猫急性失血性休克有疗效。
[Abstract]:Blood transfusion therapy is a method of being treated by importing blood or blood components from healthy animals. When blood or blood component is lost, blood transfusion therapy has been widely used in clinical diagnosis and treatment. Heterogenous blood transfusion is a kind of blood transfusion between animals, including whole blood, blood components and blood products. In this paper, the feasibility of xenotransfusion of canine blood from cats and the observation of its curative effect on acute hemorrhagic shock in cats were studied, thus providing guidance for its clinical application in pets. Experimental study on the feasibility of xenogeneic transfusion of canine blood from cats the feasibility of xenogeneic transfusion of canine blood from cats was studied in this experiment. Eighteen healthy adult cats with an average body weight of (4 卤0.5) kg were randomly divided into 3 groups with 6 cats in each group. All test cats were identified by ear labeling method. Control group A (intravenous infusion of normal saline), test group B (intravenous infusion of whole cat blood), group C (intravenous infusion of whole dog blood), the amount of input is 20% of the total blood volume of the tested animals. During the experiment, the clinical manifestations of the cats in each group were observed, and the hematology, serum biochemistry, serum electrolyte and urine were compared for 2 hours before the experiment, 1 hour after the experiment and 2 hours after the experiment for 7 days, and the feasibility of xenogeneic transfusion of dog blood was evaluated. The results showed that there was no significant difference between group B, group C and group A (p0.05). Establishment of acute hemorrhagic shock model in cats. Nine healthy adult cats with average body weight of (4 卤0.5) kg were used to establish acute hemorrhagic shock model by cardiac bleeding. Methods: the experimental cats were treated with cardiac bleeding for 20 ml/kg after sedation. The clinical symptoms of the cats were observed during the experiment. The mental state of the experimental cats was compared at 30 min (T01) before the model preparation and 30 min (T30) at 15 min (T15) immediately after bloodletting. Body temperature, respiration, heart rate, pulse, color of visible mucosa, appetite, feces and urine. Results: a total of 9 cats were successfully established acute hemorrhagic moderate shock model. After blood loss, the cats had symptoms such as hypothermia, rapid heart rate, pale visible mucosal color, mental disorder, decreased feces and urine, and so on. Compared with 30 min before model preparation (T01), 30 min after bloodletting (T30), cat lymphocytes, monocytes, neutrophils, erythrocytes, hemoglobin, hematocrit significantly decreased beyond the normal range. The difference was significant (P0.05). The content of Na + K + Cl- was significantly decreased (P0.05), and the blood pressure was significantly decreased (P0.05). The results showed that the model of acute hemorrhagic shock could be established by using the method of cardiac bleeding. Experimental observation on the therapeutic effect of xenotransfusion of canine blood on acute hemorrhagic shock in cats the purpose of this study was to investigate the therapeutic effect of xenotransfusion of canine blood on acute hemorrhagic shock in cats. Nine cats with successful modeling of acute hemorrhagic shock in test 鈪，

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