含β-内酰胺类药物有抗奶对犊牛生长发育和血液免疫指标的影响

发布时间：2018-08-07 12:49

【摘要】：试验旨在研究饲喂含β-内酰胺类药物有抗奶对荷斯坦公犊牛生长发育和血液免疫指标的影响。选用3日龄、体重(42.64kg±1.16kg)相近的公犊牛18头,随机分为2组。对照组饲喂正常牛奶,试验组饲喂有抗奶,犊牛60日龄断奶,试验期为180d。试验第10、20、30、40和50天分别采集正常牛奶和有抗奶,测定乳成分、大肠杆菌数和乳中抗生素含量。试验第30、60、90和180天逐头空腹称重,测量体尺,并计算平均日增重(ADG)。试验第7、15、30、60、90和180天于晨饲前对公犊牛空腹静脉采血,制备血清,测定血清免疫指标。结果显示:(1)试验牛场有抗奶中β-内酰胺类抗生素含量为268.65μg/L,且有抗奶的乳蛋白含量、体细胞数(SCC)和大肠杆菌数显著高于正常牛奶(P0.05);(2)饲喂有抗奶显著降低公犊牛第30天时的体重和试验60d内的ADG(P0.05),显著降低公犊牛第90天时的体长及第60和90天时的胸围(P0.05);(3)与对照组相比,试验组公犊牛IgA在第30、60和90天时显著升高(P0.05),IgM在第7天时显著升高(P0.05),IgG在第30和60天时显著升高(P0.05);IL-2和TNF-α在第15和30天时均显著升高(P0.05)。结果提示,饲喂有抗奶明显降低犊牛哺乳前期日增重,且在一定程度上提高犊牛断奶前后的血清免疫指标水平。
[Abstract]:The aim of this study was to study the effects of anti-milk containing 尾 -lactam on growth and blood immunity of Holstein male calves. Eighteen male calves of 3 days old with similar body weight (42.64kg 卤1.16kg) were randomly divided into 2 groups. The control group was fed with normal milk, the experimental group was fed with anti-milk, the calves were weaned at 60 days old for 180 days. On the 10th day of the experiment, the normal milk and the resistant milk were collected for the determination of the composition of milk, the number of Escherichia coli and the content of antibiotics in milk. On the 30th day, 60,90th and 180th day, the weight was weighed on an empty stomach, the body gauge was measured and the average daily gain (ADG).) was calculated. The fasting venous blood was collected from the male calves before morning feeding on the 7th day of the experiment. The serum was prepared and the immunological indexes of the serum were measured. The results showed that: (1) the content of 尾 -lactam antibiotics in anti-milk was 268.65 渭 g / L, and the milk protein content of anti-milk was 268.65 渭 g / L. The number of somatic cells (SCC) and Escherichia coli were significantly higher than that of normal milk (P0.05); (2). The body weight of male calves was significantly decreased after 30 days of feeding with anti milk and the body length of male calves at day 90 and the chest circumference at day 60 and 90 were significantly decreased (P0.05). (P0.05); (3) compared with the control group, In the test group, the IgA of male calves increased significantly on the 30th and 90th day (P0.05), and the IgA of the male calves increased significantly on the 7th day (P0.05) (P0.05) on the 30th and 60th day (P0.05), and the levels of IL-2 and TNF- 伪 increased significantly on the 15th and 30th days (P0.05). The results showed that feeding with anti-milk significantly decreased the daily gain of calves in the early stage of lactation, and to some extent increased the level of serum immune indexes before and after weaning of calves.
【作者单位】：黑龙江八一农垦大学动物科技学院;上海光明荷斯坦牧业有限公司;
【基金】：基金项目:垦区奶牛提质增效关键技术研究集成与示范(HNK135-04-02)
【分类号】：S823

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