不同抗氧化剂抗氧化性能的比较及其对小鼠采食量影响的研究
发布时间：2018-08-07 17:09
【摘要】：饲料中富含的多种营养物质(维生素、脂质及蛋白等)在加工、储存、运输特别是高温环境条件下,容易被氧化导致饲料酸败,影响饲料的适口性和营养价值。饲料中添加适量的抗氧化剂能有效防止上述现象的发生,还能清除体内多余的自由基,缓解动物的氧化应激,起到抗炎、抗病等效果,提高动物机体的抗氧化性能。近年来研究发现,抗氧化剂还可以通过清除下丘脑ROS水平,提高下丘脑促采食神经元AgRP/NPY的蛋白表达,促进动物的采食。为了筛选出能够有效调控动物采食量的抗氧化剂,并研究其作用机制,本文采用体外抗氧化试验(比较抗氧化剂清除DPPH自由基和羟自由基的能力)筛选出具有较强清除自由基能力的抗氧化剂,同时用昆明小鼠作为试验动物,采用筛选出的抗氧化性能较强的抗氧化剂进行饲养试验,进一步筛选出能提高小鼠采食量的抗氧化剂,在此基础上研究其对小鼠体内抗氧化相关指标的影响以及提高小鼠采食量的调节机制,为抗氧化剂在生产中的推广运用提供试验依据。研究结果表明:(1)在本试验备选的抗氧化剂中,迷迭香酸的清除DPPH自由基和羟自由基能力比VC强,原花青素、咖啡酸和丁香酸的清除DPPH自由基和清除羟自由基能力与VC相当。(2)日粮中添加一定剂量的咖啡酸和丁香酸能显著提高小鼠的日均采食量,0.5%原花青素能显著促进小鼠的平均日增重。迷迭香酸对小鼠的日均采食量和平均日增重无显著影响。(3)日粮中添加0.5%原花青素、0.3%咖啡酸均能显著提高小鼠的日均采食量,但对平均日增重无显著影响。0.5%原花青素+0.3%咖啡酸能显著提高小鼠的平均采食量和日增重,并能显著降低小鼠下丘脑ROS水平;促进促采食神经元AgRP/NPY的蛋白表达,提示原花青素和咖啡酸能通过降低小鼠下丘脑ROS影响下丘脑神经元AgRP/NPY的表达,提高小鼠的采食量。(4)日粮中添加0.5%原花青素、0.3%咖啡酸和0.5%原花青素+0.3%咖啡酸对小鼠机体内抗氧化系统的主要指标无显著影响。综上所述,原花青素和咖啡酸有较强的抗氧化能力,且日粮中添加0.5%原花青素和0.3%咖啡酸对小鼠体内抗氧化能力无显著影响,但能通过清除小鼠下丘脑ROS水平提高AgRP/NPY的蛋白表达水平,促进小鼠采食。
[Abstract]:Many kinds of nutrients (vitamins, lipids and proteins), which are rich in feed, are easy to be oxidized in processing, storage and transportation, especially in high temperature environment, which affect the palatability and nutritional value of feed. The addition of appropriate amount of antioxidants in the feed can effectively prevent the occurrence of the above phenomena, eliminate the excess free radicals in the body, relieve the oxidative stress of animals, play the effects of anti-inflammation and anti-disease, and improve the antioxidant performance of the animal body. In recent years, it has been found that antioxidants can also increase the protein expression of AgRP/NPY in hypothalamic feeding neurons by clearing the level of hypothalamus ROS, and promote the feeding of animals. In order to screen antioxidants that can effectively regulate animal feed intake, and to study its mechanism, In this paper, antioxidants with strong scavenging ability of DPPH and hydroxyl radicals were screened by antioxidant test in vitro, and Kunming mice were used as experimental animals. The selected antioxidants with strong antioxidant properties were used to carry out feeding experiments to further screen the antioxidants that could increase the feed intake of mice. On this basis, the effects of antioxidants on the antioxidant related indexes in mice and the regulation mechanism of increasing the intake in mice were studied, which provided the experimental basis for the popularization and application of antioxidants in production. The results showed that: (1) among the antioxidants in this experiment, rosemary acid had stronger scavenging ability of DPPH free radical and hydroxyl radical than VC, proanthocyanidins, The scavenging effect of caffeic acid and eugenic acid on DPPH free radical and hydroxyl radical was similar to that of VC. (2) addition of caffeic acid and eugenic acid to diet could significantly increase the average daily gain of mice by 0.5% proanthocyanidins. Rosemary acid had no significant effect on daily intake and average daily gain of mice. (3) 0.5% proanthocyanidin 0.3wt% caffeic acid could significantly increase the daily intake of mice. But there was no significant effect on average daily gain. 0.5% procyanidin 0.3% caffeic acid could significantly increase the average intake and daily gain of mice, decrease the level of ROS in the hypothalamus of mice, and promote the protein expression of AgRP/NPY in the feeding neurons. These results suggest that proanthocyanidins and caffeic acid can affect the expression of AgRP/NPY in hypothalamic neurons by decreasing ROS in hypothalamus of mice. (4) the supplementation of 0.5% procyanidin 0.3% caffeic acid and 0.5% procyanidin 0.3% caffeic acid had no significant effect on the main indexes of antioxidant system in mice. In conclusion, proanthocyanidins and caffeic acid had strong antioxidant activity, and the supplementation of 0.5% procyanidin and 0.3% caffeic acid in diet had no significant effect on the antioxidant capacity of mice. But it can improve the expression of AgRP/NPY protein by eliminating the ROS level of hypothalamus of mice and promote the food intake of mice.
【学位授予单位】：华南农业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S816
，
本文编号：2170753
[Abstract]:Many kinds of nutrients (vitamins, lipids and proteins), which are rich in feed, are easy to be oxidized in processing, storage and transportation, especially in high temperature environment, which affect the palatability and nutritional value of feed. The addition of appropriate amount of antioxidants in the feed can effectively prevent the occurrence of the above phenomena, eliminate the excess free radicals in the body, relieve the oxidative stress of animals, play the effects of anti-inflammation and anti-disease, and improve the antioxidant performance of the animal body. In recent years, it has been found that antioxidants can also increase the protein expression of AgRP/NPY in hypothalamic feeding neurons by clearing the level of hypothalamus ROS, and promote the feeding of animals. In order to screen antioxidants that can effectively regulate animal feed intake, and to study its mechanism, In this paper, antioxidants with strong scavenging ability of DPPH and hydroxyl radicals were screened by antioxidant test in vitro, and Kunming mice were used as experimental animals. The selected antioxidants with strong antioxidant properties were used to carry out feeding experiments to further screen the antioxidants that could increase the feed intake of mice. On this basis, the effects of antioxidants on the antioxidant related indexes in mice and the regulation mechanism of increasing the intake in mice were studied, which provided the experimental basis for the popularization and application of antioxidants in production. The results showed that: (1) among the antioxidants in this experiment, rosemary acid had stronger scavenging ability of DPPH free radical and hydroxyl radical than VC, proanthocyanidins, The scavenging effect of caffeic acid and eugenic acid on DPPH free radical and hydroxyl radical was similar to that of VC. (2) addition of caffeic acid and eugenic acid to diet could significantly increase the average daily gain of mice by 0.5% proanthocyanidins. Rosemary acid had no significant effect on daily intake and average daily gain of mice. (3) 0.5% proanthocyanidin 0.3wt% caffeic acid could significantly increase the daily intake of mice. But there was no significant effect on average daily gain. 0.5% procyanidin 0.3% caffeic acid could significantly increase the average intake and daily gain of mice, decrease the level of ROS in the hypothalamus of mice, and promote the protein expression of AgRP/NPY in the feeding neurons. These results suggest that proanthocyanidins and caffeic acid can affect the expression of AgRP/NPY in hypothalamic neurons by decreasing ROS in hypothalamus of mice. (4) the supplementation of 0.5% procyanidin 0.3% caffeic acid and 0.5% procyanidin 0.3% caffeic acid had no significant effect on the main indexes of antioxidant system in mice. In conclusion, proanthocyanidins and caffeic acid had strong antioxidant activity, and the supplementation of 0.5% procyanidin and 0.3% caffeic acid in diet had no significant effect on the antioxidant capacity of mice. But it can improve the expression of AgRP/NPY protein by eliminating the ROS level of hypothalamus of mice and promote the food intake of mice.
【学位授予单位】：华南农业大学
【学位级别】：硕士
【学位授予年份】：2016
【分类号】：S816
，
本文编号：2170753
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