苦参碱和氧化苦参碱对奶牛子宫内膜炎致病菌体外抑制试验
发布时间:2018-08-11 10:21
【摘要】:为了确定苦参碱和氧化苦参碱对奶牛子宫内膜炎致病菌的体外抑菌活性,选取奶牛子宫内膜炎致病菌大肠埃希菌和金黄色葡萄球菌为供试菌株,采用纸片扩散法、牛津杯法对2种生物碱进行了药敏试验,采用液体倍比稀释法测定了其最小抑菌浓度(MIC)。结果表明,苦参碱和氧化苦参碱对奶牛子宫中分离的耐药性大肠埃希菌和耐甲氧西林金黄色葡萄球菌(MRSA)均有一定的抑制作用。苦参碱对耐药性大肠埃希菌和MRSA的MIC分别为12.5 mg/mL和25 mg/mL,氧化苦参碱对耐药性大肠埃希菌和MRSA的MIC分别为25mg/mL和50mg/mL。
[Abstract]:In order to determine the antibacterial activity of matrine and oxymatrine against the pathogenic bacteria of dairy cow endometritis in vitro, Escherichia coli and Staphylococcus aureus were selected as the test strains. The drug sensitivity of two alkaloids was tested by Oxford Cup method. The minimal inhibitory concentration (MIC).) of two alkaloids was determined by liquid doubling dilution method. The results showed that both matrine and oxymatrine could inhibit the resistance of Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA) isolated from the uterus of dairy cattle. The MIC of matrine to drug-resistant Escherichia coli and MRSA were 12.5 mg/mL and 25 mg / mL, respectively, and the MIC of oxymatrine to drug-resistant Escherichia coli and MRSA were 25mg/mL and 50 mg / mL, respectively.
【作者单位】: 北方民族大学生物科学与工程学院;国家民委发酵酿造工程生物技术重点实验室;宁夏大学农学院;
【基金】:宁夏自然科学基金项目(NZ12202)
【分类号】:S858.23
[Abstract]:In order to determine the antibacterial activity of matrine and oxymatrine against the pathogenic bacteria of dairy cow endometritis in vitro, Escherichia coli and Staphylococcus aureus were selected as the test strains. The drug sensitivity of two alkaloids was tested by Oxford Cup method. The minimal inhibitory concentration (MIC).) of two alkaloids was determined by liquid doubling dilution method. The results showed that both matrine and oxymatrine could inhibit the resistance of Escherichia coli and methicillin-resistant Staphylococcus aureus (MRSA) isolated from the uterus of dairy cattle. The MIC of matrine to drug-resistant Escherichia coli and MRSA were 12.5 mg/mL and 25 mg / mL, respectively, and the MIC of oxymatrine to drug-resistant Escherichia coli and MRSA were 25mg/mL and 50 mg / mL, respectively.
【作者单位】: 北方民族大学生物科学与工程学院;国家民委发酵酿造工程生物技术重点实验室;宁夏大学农学院;
【基金】:宁夏自然科学基金项目(NZ12202)
【分类号】:S858.23
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