抗菌肽对脂多糖导致的仔猪肝脏氧化应激的影响

发布时间：2018-08-17 09:36

【摘要】：试验旨在研究给药抗菌肽CJH对由细菌脂多糖导致的仔猪肝脏氧化应激的影响。试验选用体重、日龄相近的健康屯昌仔猪(28日龄,♂)18头,随机分为3组,每组6个重复。分别为对照组、LPS组和CJH+LPS组,其中LPS组和CJH+LPS组在试验第2 d和第14 d腹腔注射LPS,CJH+LPS组在试验第2、6、10、14 d注射抗菌肽CJH,其他各组均以注射等量生理盐水替代,试验为期14 d。结果表明:与对照组相比,LPS组免疫器官指数显著降低(P0.05),而CJH+LPS组与对照组差异不显著,与对照组相比,LPS组肝脏形态受损明显,GSH-Px、T-SOD、CAT活性显著降低(P0.05),MDA浓度及炎性因子TNF-α水平极显著升高(P0.01),IL-1β、IL-6水平显著提高(P0.05),而CJH+LPS组改善了肝脏病理形态,显著缓解了LPS导致的上述指标的异常升高,且与对照组相比均达到差异不显著水平。结果提示抗菌肽CJH可能通过缓解炎症,对LPS导致的肝脏氧化应激具有明显的改善作用。
[Abstract]:The aim of this study was to study the effect of antimicrobial peptide CJH on liver oxidative stress induced by bacterial lipopolysaccharide (LPS) in piglets. Eighteen healthy Tunchang piglets (28 days old) with similar body weight and day old were randomly divided into 3 groups with 6 replicates in each group. The LPS group and CJH LPS group were injected intraperitoneally with CJH on the 2nd and 14th day, and the other groups were injected with the same amount of normal saline for 14 days. The results showed that the immune organ index in CJH LPS group was significantly lower than that in control group (P0.05), but there was no significant difference between CJH LPS group and control group. Compared with the control group, the liver morphology of lipopolysaccharide (LPS) group decreased significantly (P0.05) the concentration of MDA and the level of inflammatory factor TNF- 伪 increased significantly (P0.01), and the level of IL-1 尾 and IL-6 increased significantly (P0.05), while the pathological morphology of liver was improved in CJH LPS group. The abnormal elevation of the above indexes caused by LPS was significantly alleviated, and the difference was not significant compared with the control group. The results suggest that the antibacterial peptide CJH may ameliorate liver oxidative stress induced by LPS by alleviating inflammation.
【作者单位】：海南大学农学院;海南大学热带动物繁育与营养学实验室;
【基金】：海南省自然科学基金项目[20163066]
【分类号】：S828.5

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