苏皖地区副猪嗜血杆菌的分离鉴定与三价灭活疫苗的初步研制

发布时间：2018-08-17 13:20

【摘要】：近年来国内养猪场副猪嗜血杆菌病日益严峻,给相关猪场造成严重的损失。本研究主要对江苏省和安徽省部分养猪场的病死猪和健康猪进行副猪嗜血杆菌的分离鉴定,为副猪嗜血杆菌的防治提供流行病学数据。同时,本室初步研制副猪嗜血杆菌血清4型、5型和12型三价灭活疫苗,用BALB/c小鼠对其进行免疫保护力的评价。结果从病死猪中分离到8株副猪嗜血杆菌,其中5型3株、12型2株、14型1株及不可定型2株;从健康猪中分离到26株副猪嗜血杆菌(分离率为7.12%),其中12型10株、4型4株、11型3株、5型1株、13型1株及未定型菌株7株。初步研制的副猪嗜血杆菌三价灭活疫苗的免疫保护效果要优于商品灭活疫苗,对副猪嗜血杆菌血清4型、5型及12型具有较好的免疫保护能力。
[Abstract]:In recent years, Haemophilus paraswine disease in domestic pig farms is becoming more and more serious, causing serious losses to related pig farms. In order to provide epidemiological data for the prevention and control of Haemophilus paraporus, Haemophilus parasuis was isolated from dead and healthy pigs in some pig farms in Jiangsu Province and Anhui Province in order to provide epidemiological data for the prevention and treatment of Haemophilus paratosus. At the same time, trivalent inactivated vaccine of Haemophilus parasuis serotype 4, serotype 5 and type 12 were prepared in our laboratory, and the immune protection of the vaccine was evaluated with BALB/c mice. Results eight strains of Haemophilus suis were isolated from the dead pigs. Among them, 3 strains of serotype 5, 2 strains of type 12, 1 strain of type 14, and 2 strains of haemophilus paratosus, were isolated from the dead pigs. 26 strains of Haemophilus parasuis were isolated from healthy pigs (the isolation rate was 7.12%). Among them, 10 strains of type 12, 4 strains of type 4, 3 strains of serotype 11, 3 strains of type 5, 1 strain of serotype 13, and 7 strains of untyped strains, were isolated from healthy pigs. The primary inactivated vaccine against Haemophilus parasuis was more effective than the commercial inactivated vaccine, and had a better immune protection ability to Haemophilus parahaemophilus serotype 4, serotype 5 and serotype 12.
【作者单位】：扬州大学兽医学院江苏省动物重要疫病与人兽共患病防控协同创新中心/农业部禽用生物制剂创制重点实验室;
【分类号】：S858.28

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