高剂量高致病性猪繁殖与呼吸综合征病毒TJM-F92疫苗株对低剂量猪瘟兔化弱毒株的免疫干扰研究

发布时间：2018-08-24 17:58

【摘要】：为研究高剂量高致病性猪繁殖与呼吸综合征病毒(PRRSV)TJM-F92疫苗株对低剂量猪瘟病毒(CSFV)C株是否存在免疫干扰作用,本研究对不同方式免疫动物从体液免疫水平、免疫攻毒保护水平及病理学变化等方面进行免疫干扰评价。结果表明,高剂量PRRSV TJM-F92疫苗株与低剂量CSFV C株联合免疫组CSFV抗体水平与低剂量CSFV C株单独免疫对照组CSFV抗体水平无显著差异,联合免疫组中高剂量PRRSV TJM-F92株未抑制低剂量CSFV C株抗体生成;攻击CSFV石门血毒后,联合免疫组与低剂量CSFV C株单独免疫对照组均未出现猪瘟临床症状,组织器官病理变化轻微,两组免疫组均可对CSFV石门系血毒攻击产生良好的保护效果。阴性对照组实验动物均表现出猪瘟明显临床症状并全部死亡。本研究表明,高剂量PRRSV TJM-F92株对低剂量CSFV C株无免疫干扰作用。本研究为PRRSV免疫防制措施的制定提供了实验依据。
[Abstract]:In order to study the immune interference of high dose and high pathogenic porcine reproductive and respiratory syndrome virus (PRRSV) TJM-F92) vaccine strain to low dose swine fever virus (CSFV) C) strain, we studied the humoral immunity of different immunized animals. The immune interference was evaluated in the level of protection and pathological changes of immune attack. The results showed that there was no significant difference in the level of CSFV antibody between the high dose PRRSV TJM-F92 vaccine strain and the low dose CSFV C strain immunized group and the low dose CSFV C strain alone immunized control group. The antibody production of low dose PRRSV TJM-F92 strain was not inhibited in the combined immunization group, neither the combined immunization group nor the low dose CSFV C alone immunized control group showed any clinical symptoms of CSFV, and the pathological changes of tissues and organs were slight. Two groups of immune groups can produce good protective effect against CSFV stone portal blood toxicity attack. All the experimental animals in the negative control group showed obvious clinical symptoms and died. The results showed that high dose PRRSV TJM-F92 strain had no immune interference on low dose CSFV C strain. This study provides experimental basis for the formulation of immune control measures for PRRSV.
【作者单位】：中国农业科学院特产研究所;华威特(北京)生物科技有限公司;中国兽医药品监察所;
【基金】：基金项目:新型重大动物疫病疫苗与诊断试剂创制及生产工艺创新(2011AA10A213)
【分类号】：S852.651

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