腹水综合征肉鸡肺组织结构和血管张力以及TRPC表达的变化

发布时间：2018-08-26 21:42

【摘要】：为了研究肉鸡腹水综合征(ascites syndrome,AS)肉鸡的肺组织、肺动脉血管壁结构重塑、肺主动脉张力的变化与规范瞬时受体电位离子通道(canonical transient receptor potential channel,TRPC)的mRNA表达情况之间的关系,采用静脉注射纤维素颗粒的方法诱发肉鸡腹水征。然后,采用HE染色和Weiger套Van-Giesen弹力纤维染色法观察肺组织结构变化,Wire-myograph系统测量KCl刺激后血管张力的变化,RT-PCR方法检测肺组织中TRPC mRNA的表达。结果显示:静脉注射纤维素颗粒诱导的腹水肉鸡肺组织间隙增宽,肺实质炎性细胞浸润及肺动脉血管壁结构重塑,且血管张力下降,但是TRPC mRNA在肺组织中的表达没有发生明显变化。研究结果表明,肉鸡腹水综合征所引起肺动脉血管壁结构重塑以及肺动脉张力的变化与TRPC mRNA的表达无关。
[Abstract]:In order to study the relationship between lung tissue, pulmonary artery wall remodeling, changes of pulmonary aortic tension and mRNA expression of normal transient receptor potential ion channel (canonical transient receptor potential channel,TRPC) in broilers with ascites syndrome (ascites syndrome,AS). Ascites were induced by intravenous injection of cellulose granules in broilers. Then, the changes of lung tissue structure were observed by HE staining and Van-Giesen elastic fiber staining with Weiger. The changes of vascular tension after KCl stimulation were measured by Wire-myograph system. RT-PCR was used to detect the expression of TRPC mRNA in lung tissue. The results showed that the interstitial space was widened, inflammatory cell infiltration of pulmonary parenchyma and remodeling of pulmonary artery wall were induced by intravenously injected cellulose granules in broilers with ascites, and the vascular tension was decreased. However, there was no significant change in the expression of TRPC mRNA in lung tissue. The results showed that the remodeling of pulmonary artery wall and the change of pulmonary artery tension were not related to the expression of TRPC mRNA in broiler ascites syndrome.
【作者单位】：华南农业大学兽医学院;
【基金】：国家自然科学青年基金项目(31502131) 十三五国家重点研发计划项目(2016YFD0501205)
【分类号】：S858.31

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